The dual antimicrobial and immunomodulatory roles of host defense peptides and their applications in animal production

Wu, Di; Fu, Linglong; Wen, Weizhang; Dong, Na

doi:10.1186/s40104-022-00796-y

Cited by 22 publications

(13 citation statements)

References 132 publications

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“…Cationic AMPs are believed to possess multifaceted immunomodulatory properties along with their ability to inhibit microbes directly. The ability of the AMPs to elicit a pro-inflammatory response for pathogen clearance along with an anti-inflammatory reaction to suppress the adverse over-inflammatory effects becomes vital for the maintenance of immune homeostasis . As the FLPII motif has been reported to possess immunomodulatory attributes, the same was checked in the U937 cell line.…”

Section: Resultsmentioning

confidence: 99%

“…The ability of the AMPs to elicit a pro-inflammatory response for pathogen clearance along with an anti-inflammatory reaction to suppress the adverse overinflammatory effects becomes vital for the maintenance of immune homeostasis. 33 As the FLPII motif has been reported to possess immunomodulatory attributes, the same was checked in the U937 cell line. In LPS-stimulated cells, the expression of the pro-inflammatory cytokines IL-6 and TNF-α was understandably high, indicating a heightened immune response (Figure 3C).…”

Section: Preliminary Inhibition Study Of the Synthesizedmentioning

confidence: 99%

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Potential Broad-Spectrum Antimicrobial, Wound Healing, and Disinfectant Cationic Peptide Crafted from Snake Venom

Sen,

Samat,

Jash

et al. 2023

J. Med. Chem.

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Antimicrobial cationic peptides are intriguing and propitious antibiotics for the future, even against multidrug-resistant superbugs. Venoms serve as a source of cutting-edge therapeutics and innovative, unexplored medicines. In this study, a novel cationic peptide library consisting of seven sequences was designed and synthesized from the snake venom cathelicidin, batroxicidin (BatxC), with the inclusion of the FLPII motif at the N-terminus. SP1V3_1 demonstrated exceptional antibacterial effectiveness against Escherichia coli, methicillin-resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa, and Klebsiella pneumoniae and destroyed the bacteria by depolarizing, rupturing, and permeabilizing their membranes, as evident from fluorescence assays, atomic force microscopy, and scanning electron microscopy. SP1V3_1 was observed to modulate the immune response in LPS-elicited U937 cells and exhibited good antibiofilm activity against MRSA and K. pneumoniae. The peptide promoted wound healing and disinfection in the murine model. The study demonstrated that SP1V3_1 is an exciting peptide lead and may be explored further for the development of better therapeutic peptides.

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Section: Resultsmentioning

confidence: 99%

Section: Preliminary Inhibition Study Of the Synthesizedmentioning

confidence: 99%

Potential Broad-Spectrum Antimicrobial, Wound Healing, and Disinfectant Cationic Peptide Crafted from Snake Venom

Sen,

Samat,

Jash

et al. 2023

J. Med. Chem.

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“…The bacterial clearance in the host depends on the migration of phagocytes into infected sites [13,14]. To investigate whether PMAP-36 in combination with tetracycline promotes the recruitment of immune cells, number of monocytes and macrophages was determined in the PLF.…”

Section: Pmap-36 In Combination With Tetracycline Promotes Monocytes/...mentioning

confidence: 99%

“…6A and B, PMAP-36 and tetracycline alone treatment only slightly increased the number of monocytes and macrophages but PMAP-36 and tetracycline combination signi cantly increased the recruitment of cells, suggesting that the combination of PMAP-36 and tetracycline promotes the recruitment of phagocytes to the infected sites. [14]. Next, we investigated whether PMAP-36 combined with tetracycline promoted chemokines secretion to induce immune cells migration.…”

Section: Pmap-36 In Combination With Tetracycline Promotes Monocytes/...mentioning

confidence: 99%

Antibacterial activity of antimicrobial peptide PMAP-36 combined with tetracycline against porcine extraintestinal pathogenic Escherichia coli in vitro and in vivo

Tao,

Lu,

Liu

et al. 2023

Preprint

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The increased emergence of antimicrobial resistance brings great challenges in controlling porcine extraintestinal pathogenic Escherichia coli(ExPEC) infections. Combinations of antimicrobial peptides (AMPs) and antibiotics can synergistically improve antimicrobial efficacy and reduce bacterial resistance. This study investigated the antibacterial activity of porcine myeloid antimicrobial peptide 36 (PMAP-36) in combination with tetracycline against porcine ExPEC PCN033 both in vitroand in vivo. We found that the MBCs of AMPs (PMAP-36 and PR-39) against ExPEC strains PCN033 and RS218 were 10 μM and 5 μM, respectively. PMAP-36 and antibiotics (tetracycline, gentamicin) had a synergistic bactericidal effect against PCN033. PMAP-36 delayed the emergence of PCN033 resistance to tetracycline. The treatment of PMAP-36 combined with tetracycline significantly increased survival and reduced bacterial load as well as decreasing inflammatory response in mice. In addition, the combination of PMAP-36 and tetracycline promoted the migration of monocytes/macrophages to the infection site. Our results suggest that AMPs in combination with antibiotics may provide more therapeutic options against multi-drug resistant porcine ExPEC.

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“…Consequently, the exploration of alternative approaches, such as antimicrobial peptides, has gained prominence as a potential solution to counter this predicament ( 1 , 2 ). Antimicrobial peptides (AMPs) represent a class of small molecular polypeptides consisting of 10–60 amino acids and are integral components of the innate immunity found in animals and plants ( 3 – 5 ). Termed host defense peptides, AMPs have garnered significant attention and are cataloged in the antimicrobial peptide database (APD3), the most extensive repository of antimicrobial peptides, comprising 3,569 peptides from diverse life kingdoms, including those synthesized through predictive approaches.…”

Section: Introductionmentioning

confidence: 99%

BsR1, a broad-spectrum antibacterial peptide with potential for plant protection

Song,

Zhao,

Zhu

et al. 2023

Microbiol Spectr

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The urgent need for new antibacterial drugs arises from the emergence of bacterial multidrug resistance due to antibiotic overuse. Antimicrobial peptides, crucial components of innate immunity in animals and plants, exhibit effectiveness against multidrug-resistant bacteria while minimizing the development of drug resistance. In this study, the antibacterial peptide BsR1, comprising 21 amino acids, was finalized by predicting the active site of an antifungal gene and sequence optimization. BsR1 displayed broad-spectrum antibacterial activities against diverse Gram-positive and Gram-negative bacteria, with a low minimum inhibitory concentration of 4.25–17 μM. Stability experiments demonstrated that BsR1 has high resistance to thermal, ultraviolet, and acid-base conditions, while revealing increased sensitivity to divalent ions Ca 2+ and Mg 2+ . The mode of action of BsR1 involved cell membrane damage, leading to bacterial cell structure disruption and subsequent death. Secondary structure prediction indicated a linear helical conformation with a positive charge of +7.5, facilitating its interaction with the target cell membrane. BsR1 exhibited excellent biological safety, as it did not induce necrosis in tobacco leaves, and the low observed hemolytic effect on mammalian cells with a value of 3.26%. Additionally, BsR1 demonstrated the ability to enhance disease resistance in rice and effectively curbed the spread of rice bacterial blight. This research presents BsR1 as a novel approach and potential medication in the development of antibacterial drugs, offering a valuable tool in combating pathogenic microorganisms, particularly in plants. IMPORTANCE This study addresses the critical need for new antibacterial drugs in the face of bacterial multidrug resistance resulting from antibiotic overuse. It highlights the significance of antimicrobial peptides as essential components of innate immunity in animals and plants, which have been proven effective against multidrug-resistant bacteria and are difficult to develop resistance against. This study successfully synthesizes a broad-spectrum antibacterial peptide, BsR1, with strong inhibitory activities against various Gram-positive and Gram-negative bacteria. BsR1 demonstrates favorable stability and a mode of action that damages bacterial cell membranes, leading to cell death. It also exhibits biological safety and shows potential in enhancing disease resistance in rice. This research offers a novel approach and potential medication for antibacterial drug development, presenting a valuable tool in combating pathogenic microorganisms, particularly in plants.

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The dual antimicrobial and immunomodulatory roles of host defense peptides and their applications in animal production

Cited by 22 publications

References 132 publications

Potential Broad-Spectrum Antimicrobial, Wound Healing, and Disinfectant Cationic Peptide Crafted from Snake Venom

Potential Broad-Spectrum Antimicrobial, Wound Healing, and Disinfectant Cationic Peptide Crafted from Snake Venom

Antibacterial activity of antimicrobial peptide PMAP-36 combined with tetracycline against porcine extraintestinal pathogenic Escherichia coli in vitro and in vivo

BsR1, a broad-spectrum antibacterial peptide with potential for plant protection

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