The dual probiotic and antibiotic nature of Bdellovibrio bacteriovorus

Dwidar, Mohammed; Monnappa, Ajay K.; Mitchell, Robert J.

doi:10.5483/bmbrep.2012.45.2.71

Cited by 137 publications

(154 citation statements)

References 54 publications

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“…Then, it occupies the periplasmic space and undergoes growth phase which culminates in prey killing and release of progeny (4). B. bacteriovorus is ubiquitous both in aquatic and terrestrial habitats and has recently been discovered in the human gut, where it is alleged to act as a "probiotic" (5,6). Previous research has demonstrated that B. bacteriovorus has the potential to prey upon several human pathogens which grow in planktonic cultures or in biofilm (7).…”

Section: Introductionmentioning

confidence: 99%

Predation Efficacy of <i>Bdellovibrio bacteriovorus</i> on Multidrug-Resistant Clinical Pathogens and Their Corresponding Biofilms

Sun

Hou

et al. 2017

Jpn J Infect Dis

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Section: Introductionmentioning

confidence: 99%

Predation Efficacy of <i>Bdellovibrio bacteriovorus</i> on Multidrug-Resistant Clinical Pathogens and Their Corresponding Biofilms

Sun

Hou

et al. 2017

Jpn J Infect Dis

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“…They have also been applied in the treatment of human disease pathogens, including Pseudomonas aeruginosa from the lungs of cystic fibrosis patients (11), Pseudomonas aeruginosa and Serratia marcescens associated with eye infections (12), and oral pathogens that cause periodontal diseases (13,14). Bdellovibrio strains were also effective in the treatment of drug-resistant bacteria (15,16) and in reducing the levels of Escherichia coli and Salmonella spp. from stainless steel surfaces (17).…”

mentioning

confidence: 99%

Purification and Host Specificity of Predatory Halobacteriovorax Isolates from Seawater

Richards

Fay

Uknalis

et al. 2016

Appl Environ Microbiol

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c Halobacteriovorax (formerly Bacteriovorax) is a small predatory bacterium found in the marine environment and modulates bacterial pathogens in shellfish. Four strains of Halobacteriovorax originally isolated in Vibrio parahaemolyticus O3:K6 host cells were separated from their prey by an enrichment-filtration-dilution technique for specificity testing in other bacteria. This technique was essential, since 0.45-m filtration alone was unable to remove infectious Vibrio minicells, as determined by scanning electron microscopy and cultural methods. Purified Halobacteriovorax strains were screened for predation against other V. parahaemolyticus strains and against Vibrio vulnificus, Vibrio alginolyticus, Escherichia coli O157:H7, and Salmonella enterica serovar Typhimurium DT104, all potential threats to seafood safety. They showed high host specificity and were predatory only against strains of V. parahaemolyticus. In addition, strains of Halobacteriovorax that were predatory for E. coli O157:H7 and S. Typhimurium DT104 were isolated from a tidal river at 5 ppt salinity. In a modified plaque assay agar, they killed their respective prey over a broad range of salinities (5 to 30 ppt). Plaques became smaller as the salinity levels rose, suggesting that the lower salinities were optimal for the predators' replication. These species also showed broader host specificity, infectious against each other's original hosts as well as against V. parahaemolyticus strains. In summary, this study characterized strains of Halobacteriovorax which may be considered for use in the development of broad-based biocontrol technologies to enhance the safety of commercially marketed shellfish and other foods. Bdellovibrio and like organisms (BALO) are Gram-negative, aerobic bacteria which are predatory toward other Gram-negative bacteria and consist of freshwater/terrestrial and marine forms. Bdellovibrio and like organisms are divided into four genera, Bdellovibrio, Bacteriolyticum, Peredibacter, and Bacteriovorax, which was recently renamed Halobacteriovorax (1). These organisms enter into a susceptible host bacterium and reside within the periplasmic space where they utilize the cytoplasmic nutrients of the host to support growth and replication. The replicative form, known as a bdelloplast, elongates within the host and septates into progeny cells as the host is lysed, releasing cells capable of attacking more prey. Halobacteriovorax organisms are the marine forms, which are small, polar flagellated, highly motile, intracellular predators in the class Deltaproteobacteria family Halobacteriovoraceae (1).Bdellovibrio and like organisms, particularly the nonhalophilic Bdellovibrio bacteriovorus, have been proposed for use in reducing the levels of phytopathogens associated with bacterial blights in soybeans and rice (2, 3) and reducing the levels of Salmonella enterica serovar Typhimurium on freshwater fish fillets (4). Other uses involve the treatment of various infections in animals, including bovine keratoconjunctivitis caused by Mora...

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“…Furthermore, increasing antibiotic resistance among Gram-negative pathogens has evoked new interest in the potential use of predatory bacteria for therapeutics (4,5). Throughout the years, several methods were used to measure predation and predator activity.…”

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confidence: 99%

Visualizing Bdellovibrio bacteriovorus by Using the tdTomato Fluorescent Protein

Mukherjee

Shanks

Kadouri

2016

Appl Environ Microbiol

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bBdellovibrio bacteriovorus is a Gram-negative bacterium that belongs to the delta subgroup of proteobacteria and is characterized by a predatory life cycle. In recent years, work has highlighted the potential use of this predator to control bacteria and biofilms. Traditionally, the reduction in prey cells was used to monitor predation dynamics. In this study, we introduced pMQ414, a plasmid that expresses the tdTomato fluorescent reporter protein, into a host-independent strain and a host-dependent strain of B. bacteriovorus 109J. The new construct was used to conveniently monitor predator proliferation in real time, in different growth conditions, in the presence of lytic enzymes, and on several prey bacteria, replicating previous studies that used plaque analysis to quantify B. bacteriovorus. The new fluorescent plasmid also enabled us to visualize the predator in liquid cultures, in the context of a biofilm, and in association with human epithelial cells.

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The dual probiotic and antibiotic nature of Bdellovibrio bacteriovorus

Cited by 137 publications

References 54 publications

Predation Efficacy of <i>Bdellovibrio bacteriovorus</i> on Multidrug-Resistant Clinical Pathogens and Their Corresponding Biofilms

Predation Efficacy of <i>Bdellovibrio bacteriovorus</i> on Multidrug-Resistant Clinical Pathogens and Their Corresponding Biofilms

Purification and Host Specificity of Predatory Halobacteriovorax Isolates from Seawater

Visualizing Bdellovibrio bacteriovorus by Using the tdTomato Fluorescent Protein

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