The Duodenal Secretion in Man as a Function of Secretin Dose and Secretin Inactivation

Lagerlof, H; Ek, Stig; Nyberg, A.

doi:10.1016/s0016-5085(19)35021-8

Cited by 21 publications

(5 citation statements)

References 11 publications

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“…5 = 4-06 min) for man, who all measured secretin by radioimmunoassay. Based on the pancreatic secretion the half-life of secretin has been shown to be 3-2 min in the dog (Lehnert, Stahlheber, Forrell, Dost, Fritz, Hutzel & Werte, 1969) and 3-3 min in the cat (Clark, Greenwell, Harper, Sankey &Scratcherd, 1967), andLagerlöf, Ek &Nyberg (1962) . Results from gel-permeation chromatography on a Sephadex G-50 Superfine column (9 mm x 1000 mm) eluted with NH"HCO, (0·25 mol/1) containing 72·5 μπιοΐ of human serum albumin and 200000 i.ku.…”

Section: Discussionmentioning

confidence: 99%

Elimination of Porcine Secretin in Pigs

1978

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1. The elimination of immunoreactive secretin was studied in anaesthetized pigs by using constant infusions of pure natural porcine secretin. 2. The mean metabolic clearance rate was 15.4 ml min-1 kg-1, and was independent of the level at which it was determined. The mean distribution space was 64.4 ml/kg. The half-life of secretin after termination of the infusion averaged 2.6 min. 3. During intravenous infusion of secretin in a dose of 27.8 pmol h-1 kg-1 the renal extraction was 52%. Exclusion of the kidneys increased plasma secretin concentration from 26.5 pmol/l to 47.3 pmol/l and increased the half-life to 4.4 min. 4. Exclusion of the liver during infusion of secretin in a dose of 27.8 pmol h-1 kg-1 resulted in an increment in plasma secretin concentration of 7.8 pmol/l and an increase of the half-life to 3.6 min. 5. A gradient of endogenous secretin across the liver was present but no arteriovenous difference was found across the foreleg. 6. Incubation of secretin with whole blood or serum for 20 min at 37 degrees C did not result in any degradation of secretin.

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Section: Discussionmentioning

confidence: 99%

Elimination of Porcine Secretin in Pigs

1978

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“…In each of these investigations, either pure porcine or synthetic secretin was infused to determine the half-life of the hormone. Lagerlof et al (1962) calculated the half-life of secretin in man to be 17 minutes, as judged by pancreatic response.…”

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confidence: 99%

Disappearance half-time of endogenous and exogenous secretin in dogs.

Curtis¹,

Fender²,

Rayford³

et al. 1976

Gut

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SUMMARY We have used a sensitive and specific radioimmunoassay to measure the disappearance half-times of exogenous porcine secretin and endogenous canine secretin in the dog and found them to be 2-45 and 2-85 minutes, respectively.Since the discovery of secretin in 1902 by Bayliss and Starling, quantitative estimations of secretin activity have been based on the ability of the hormone to stimulate flow of bicarbonate-rich pancreatic juice. Since about the time of the initial isolation of the hormone from hog intestine by Jorpes and Mutt (1959), there have been refinements in bioassay technique (Ivy and Janecek, 1959;Mutt and Soderberg, 1959;Heatley, 1968) which have produced considerable standardisation of results. Unfortunately, these techniques lacked specificity and precision, and were incapable of measuring basal levels of secretin in the circulating plasma of animals and man.Calculations of the disappearance half-time (TI) of secretin were, however, made in laboratory animals using pancreatic secretion as an index of secretin activity (Clark et al., 1967;Lehnert et al., 1969;Hubel, 1972).We have recently validated a radioimmunoassay which is both sensitive and specific and can accurately measure concentrations of secretin of less than 20 pg/mi in plasma (Rayford et al., 1976).Using this assay technique, we have measured plasma levels of secretin in dogs after an infusion of pure natural porcine hormone and have calculated its disappearance half-time. We have also measured basal and stimulated levels of endogenous secretin in dogs and have calculated its disappearance halftime. proximately 20 kg were placed in Pavlov stands and an intravenous catheter placed in a foreleg and a hind leg of each dog. After collecting two 5 ml basal blood samples, 10 minutes apart, each dog received an infusion of pure natural secretin (Professor V.Mutt, Gastrointestinal Hormone Laboratory [GIH], Karolinska Institute, Stockholm), 1.5 unit/kg body weight, dissolved in 50 ml 0-9 % NaCI. This secretin preparation contains 3500 clinical units/mg. The infusion was delivered over 30 minutes into a foreleg vein using a Harvard syringe pump. Blood samples were collected from the hind leg catheter at five-minute intervals during the infusion. The infusion was then terminated and samples collected at one-minute intervals for 10 minutes, then at two-minute intervals for 10 minutes. DISAPPEARANCE HALF-TIME OF ENDOGENOUS SECRETINA further six fasting, adult, mongrel dogs were anaesthetized with intravenous sodium pentobarbital ,(25,mg/kg), and the abdomen opened. A segment of bowel, comprising the whole of the duodenum and the proximal 50 cm of the jejunum, was prepared for stimulation of secretin release by ligation of the pylorus, ligation of the superior pancreaticoduodenal vein at its entry into the portal vein, and division of the jejunum and its mesentery 50 cm distal to the ligament of Treitz. A cannula was introduced into the proximal duodenum for infusion of acid, and the distal end of the segment of bowel was left open to allow f...

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“…Thus, regurgitation to the stomach cannot be excluded. Lagerlof (1942) and Dreiling and Hollander (1948) observed, however, that permanent aspiration of the duodenal contents prevented regurgitation to the stomach.…”

Section: Discussionmentioning

confidence: 98%

“…2c 1934; Agren and Lagerlof, 1936;Diamond, Siegel, The calculated secretion of amylase in four Gall, and Karlen, 1939;Lagerlof, 1942;Dornberger, ? periods after stimulation of the pancreatic Comfort, Wollaeger, and Power, 1948;Pfeffer, Varley, 1960;Dreiling and Janowitz, 1962;Lagerlof, Ek and Nyberg, 1962;Correia and Barros, 1963;Christensen, 1963). Cases with the largest aspirates in the second 20-minute period following the injection of secretin have been recorded (Lake, 1947;Cathalan, Charmot, and Pujo, 1961;Gulzow and Arendt, 1964), but values in the periods 40-60 and 60-80 minutes exceeding those obtained in the initial 20 minutes have never been observed.…”

Section: Discussionmentioning

confidence: 99%

Validity of polyethyleneglycol in the study of the total pancreatic secretion in man after stimulation by secretin, pancreozymin, or food.

Worning¹

1967

Gut

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The Duodenal Secretion in Man as a Function of Secretin Dose and Secretin Inactivation

Cited by 21 publications

References 11 publications

Elimination of Porcine Secretin in Pigs

Elimination of Porcine Secretin in Pigs

Disappearance half-time of endogenous and exogenous secretin in dogs.

Validity of polyethyleneglycol in the study of the total pancreatic secretion in man after stimulation by secretin, pancreozymin, or food.

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