The dynamic nature of Bruch's membrane

Booij, Judith C.; Baas, Dominique C.; Beisekeeva, J. S.; Gorgels, Theo G. M. F.; Bergen, Arthur A. B.

doi:10.1016/j.preteyeres.2009.08.003

Cited by 492 publications

(441 citation statements)

References 139 publications

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“…Therefore, BC substrates show similar permeation coefficients relatively to the control, indicating that they allow fluid transport and are permeable to macromolecules up to a molecular weight of 300 kDa, which is the maximum that is transported by RPE cells. 6 Since permeation coefficients did not differ between BC conditions, it was expected the presence of porous structures in all BC substrates, which was confirmed through SEM analysis of BC, ABC, CBC and CMBC substrates (Figure 3). BC's porosity is an advantageous feature when comparing to other non-biodegradable biomaterials that have been proposed such as polydimethylsiloxane 57 , polypropylene 58 and the already patented for RPE transplantation parylene (patent publication number: US20120009159 A1; publication date: 12 Jan 2012).…”

Section: Methodssupporting

confidence: 52%

“…5 Located between the RPE and the fenestrated choriocapillaries, the BM is an extracellular matrix structure composed of elastin and collagen that partly regulates the reciprocal exchange of biomolecules, nutrients, oxygen, fluids and metabolic waste products between the retina and the general circulation. 6 When the RPE cells are lost and the BM is compromised in retinal degenerative diseases, treatment approaches are limited to regenerative strategies, visual prostheses (either electrical or biologic), or standard vision rehabilitation (facilitate a closer reading distance or magnify a distant image). 4,7,8 Human retinal transplantation has followed many years of experimental research showing that transplanted RPE cells have the potential to rescue photoreceptors.…”

Section: Introductionmentioning

confidence: 99%

“…2,3,10,11 A BM prosthetic substrate should be able to perform the BM's primary functions: regulation of (bio-) molecules passive diffusion between choroid and RPE; provide physical support for RPE adhesion, migration and perhaps differentiation; and act as a division barrier for cell migration. 6 In addition, these substrates should be biocompatible, able to maintain the RPE phenotype, and exhibit favorable surgical properties (handling stability and resistance to tear). 3,10 Recent works in the development of biocompatible substrates to patch or replace diseased BM for the delivery and long-term survival of RPE transplants include: polyethylene terephthalate and poly(L-lactide-co-ε-caprolactone) films and electrospun substrates 10 ; montmorillonite clay based polyurethane substrates 12 ; methacrylate-based copolymer electrospun fibrous scaffold 13 ; ultrathin and biofunctionalized polyimide membranes 7 ; and ultrathin collagen membranes.…”

Section: Introductionmentioning

confidence: 99%

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Bacterial Cellulose As a Support for the Growth of Retinal Pigment Epithelium

Gonçalves¹,

Padrão²,

Rodrigues

et al. 2015

Biomacromolecules

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The feasibility of bacterial cellulose (BC) as a novel substrate for retinal pigment epithelium (RPE) culture was evaluated. Thin (41.6 ± 2.2 μm of average thickness) and heat-dried BC substrates were surface-modified via acetylation and polysaccharide adsorption, using chitosan and carboxymethyl cellulose. All substrates were characterized according to their surface chemistry, wettability, energy, topography, and also regarding their permeability, dimensional stability, mechanical properties, and endotoxin content. Then, their ability to promote RPE cell adhesion and proliferation in vitro was assessed. All surface-modified BC substrates presented similar permeation coefficients with solutes of up to 300 kDa. Acetylation of BC decreased it's swelling and the amount of endotoxins. Surface modification of BC greatly enhanced the adhesion and proliferation of RPE cells. All samples showed similar stress-strain behavior; BC and acetylated BC showed the highest elastic modulus, but the latter exhibited a slightly smaller tensile strength and elongation at break as compared to pristine BC. Although similar proliferation rates were observed among the modified substrates, the acetylated ones showed higher initial cell adhesion. This difference may be mainly due to the moderately hydrophilic surface obtained after acetylation. 2 ABSTRACT: The feasibility of bacterial cellulose (BC) as a novel substrate for retinal pigment epithelium (RPE) culture was evaluated. Thin (41.6 ± 2.2 µm of average thickness) and heatdried BC substrates were surface modified via acetylation and polysaccharide adsorption using chitosan and carboxymethyl cellulose. The BC substrates were characterized according to surface chemistry, wettability, energy, topography, permeability, dimensional stability, mechanical properties and level of endotoxins present. Then, the ability to promote RPE cell adhesion and proliferation in vitro was assessed. BC substrates were porous and permeable to solutes up to 300 kDa. The acetylation decreased substrate swelling and the amount of endotoxins present. Surface modification greatly enhanced the adhesion and proliferation of RPE cells in BC. Although similar proliferation rates were observed between the modified substrates, the acetylated ones showed higher initial cell adhesion. This difference may be mainly due to the moderately hydrophilic surface obtained after acetylation.

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Section: Methodssupporting

confidence: 52%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Bacterial Cellulose As a Support for the Growth of Retinal Pigment Epithelium

Gonçalves¹,

Padrão²,

Rodrigues

et al. 2015

Biomacromolecules

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“…The retinal pigment epithelium (RPE) performs several complex functions essential for visual function, namely light energy absorption, photoreceptor outer segments phagocytosis, outer blood-retina barrier, secretion of immunosuppressive and growth factors [1,5]. As outer blood-retina barrier, the RPE prevents the transport of molecules larger than 300 kDa via intracellular mediation [6]. To be able to perform its complex functions, RPE cells have unique morphological and functional polarity properties, with an expression and polarized distribution of receptors, transporters, channels and enzymes (many of these are markers of differentiated RPE) [5].…”

Section: Introductionmentioning

confidence: 99%

Acetylated bacterial cellulose coated with urinary bladder matrix as a substrate for retinal pigment epithelium

Gonçalves

Rodrigues

Padrão

et al. 2016

Colloids and Surfaces B: Biointerfaces

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This work evaluated the effect of acetylated bacterial cellulose (ABC) substrates coated with urinary bladder matrix (UBM) on the behavior of retinal pigment epithelium (RPE), as assessed by cell adhesion, proliferation and development of cell polarity exhibiting transepithelial resistance and polygonal shaped-cells with microvilli. Acetylation of bacterial cellulose (BC) generated a moderate hydrophobic surface (around 65°) while the adsorption of UBM onto these acetylated substrates did not affect significantly the surface hydrophobicity. The ABS substrates coated with UBM enabled the development of a cell phenotype closer to that of native RPE cells. These cells were able to express proteins essential for their cytoskeletal organization and metabolic function (ZO-1 and RPE65), while showing a polygonal shaped morphology with microvilli and a monolayer configuration. The coated ABC substrates were also characterized, exhibiting low swelling effect (between 1.5-2.0 swelling/mm3), high mechanical strength (2048MPa) and non-pyrogenicity (2.12EU/L). Therefore, the ABC substrates coated with UBM exhibit interesting features as potential cell carriers in RPE transplantation that ought to be further explored. Therefore, the ABC substrates coated with UBM showed a great potential to be applied as cell carriers in RPE transplantation.

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“…2 Bruch's membrane separates the retinal pigment epithelium from the capillary lamina of the choroid and is important for exchange of nutrients, oxygen, and waste products between the outer retina and the circulatory system. 3,4 Late stages of AMD include geographic atrophy and choroidal neovascularization.…”

Section: Introductionmentioning

confidence: 99%

Cognitive function, drusen, and age-related macular degeneration: a cross-sectional study

Lindekleiv

Erke

Bertelsen

et al. 2013

Eye

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Purpose To examine the cross-sectional relationship between drusen, late age-related macular degeneration (AMD), and cognitive function. Methods We included 2149 stroke-free participants from the population-based Tromsø Study in Norway. Retinal photographs were graded for presence of drusen and AMD. Cognitive function was assessed using the verbal memory test (short verbal memory), digit-symbol coding test (processing speed), and the tapping test (psychomotor tempo). We assessed the relationship between drusen, late AMD, and cognitive test scores, adjusted for potential confounders. Results Late AMD was associated with decreased performance in the verbal memory test (standardized b ¼ À 0.23, 95% confidence interval (CI): À 0.51 to À 0.01). Intermediate and large drusen were associated with decreased performance in the digit-symbol coding test (standardized b ¼ À 0.14 and -0.19, 95% CIs: À 0.23 to À 0.05 and À 0.29 to À 0.09, respectively). Participants with large drusen were more likely to have test scores in the lowest quartile of the digit-symbol coding test (odds ratio (OR) ¼ 1.9, 95% CI: 1.1-3.5) and the tapping test (OR ¼ 1.6, 95% CI: 1.0-2.6), but not in the verbal memory test (OR ¼ 1.0, 95% CI: 0.6-1.6). Conclusions The findings suggest a relationship between drusen deposition and reduced cognitive function. Although the relationships between drusen, late AMD, and the cognitive test results varied in strength and significance across the types of cognitive test, and may partly have been caused by residual confounding, it is not unlikely that a genuine but weaker relationship exists between drusen deposition and cognitive decline.

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The dynamic nature of Bruch's membrane

Cited by 492 publications

References 139 publications

Bacterial Cellulose As a Support for the Growth of Retinal Pigment Epithelium

Bacterial Cellulose As a Support for the Growth of Retinal Pigment Epithelium

Acetylated bacterial cellulose coated with urinary bladder matrix as a substrate for retinal pigment epithelium

Cognitive function, drusen, and age-related macular degeneration: a cross-sectional study

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