The dynamics of humoral protection factors in broilers under the conditions of probiotic preparations application

Romanovych, М.М.

doi:10.15421/nvlvet8352

Cited by 4 publications

(3 citation statements)

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“…При додаванні пробіотиків до корму чи води кишечник заселяється корисними бактеріями, уникаючи або зменшуючи ступінь колонізації патогенів. Оскільки антибіотики вилучаються зі звичайної практики тваринництва та птахівництва, пробіотики вважаються перспективним інструментом запобігання виникненню захворювань (Nechyporenko et al, 2018;Osman et al, 2020).…”

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The use of probiotics in the cultivation of turkeys

Kytaieva

Петров

2020

SMLNUVMB

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The specific microclimate of poultry premises contributes to the accumulation of microflora, which may contain pathogenic and opportunistic microorganisms. The concentration of microorganisms in poultry houses increases over time and reduces the resistance of the bird. Researchers have reported very high growth rates of mesophilic aerobic microorganisms, which include most pathogenic bacteria, namely: gram-positive cocci (staphylococci, enterococci) and gram-negative bacteria of the family Enterobacteriaceae (Escherichia coli, Salmonella spp., Salmonella spp.). Proteus spp., Klebsiella spp., and Pseudomonas spp. The goal of our research was to investigate and compare the effectiveness of two probiotics for turkeys, one of which contains recombinant strains of lactic acid microorganisms of intestinal intestinal symbionts, and the other based on a mixture of biologically active substances based on natural components of organo-mineral origin. The task of our work: 1. To study the microflora of the gastrointestinal tract during the use of probiotics after the use of antiparasitic drugs in nematode invasion of turkeys. 2. Compare the growth of young animals with the use of probiotics and provide suggestions on the prospects for the most effective use of probiotics in the cultivation of turkeys. 3. Investigate changes in the physicochemical properties of turkey meat when using probiotics and compare them with controls. To conduct the experiment, three experimental groups of turkeys cross Hybrid converter, 14 days old, each with 10 heads. The first and second experimental groups were given an antiparasitic drug containing levamisole hydrochloride at a dose of 5 ml per 5 liters of water once. After that, probiotics were used for each experimental group of birds. Two types of probiotics were used in the experiments: the first contains recombinant strains of lactic acid microorganisms of intestinal intestinal symbionts: Bifidobacterium bifidum, Bacillus thermophilus, Bacillus coagulance, Bacillus subtilis. Has antimicrobial, antiviral and antioxidant activity; the second preparation contains a mixture of biologically active substances, based on natural ingredients of organo-mineral origin, which includes organic substances – fulvic and humic acids. The third group (control) used only the above antiparasitic drug. After the experiment, at the age of 78 days, poultry was slaughtered and studies of the effect of probiotics on weight, physicochemical parameters of meat. When testing turkeys for helminths. As a result of research, helminths were identified, which were attributed to the causative agent of heterakidosis - Heeterakis gallibarum. After that, it was decided to carry out deworming with levamisole hydrochloride. After a course of treatment, it was found that no deaths were observed in the first experimental group; in the second experimental group, one head died, and in the third control group, which did not use probiotics, the death was two heads. The use of probiotics due to competition reduces the level of total bacterial contamination and coliform bacteria in the experimental groups. And the level of lactic acid bacteria in the experimental groups was higher than in the control. The use of probiotics had a positive effect on weight gain of experimental turkeys, compared with the control group, where probiotics were not used. The difference in the first experimental group compared with the control averaged 618 g, and in the second group – 198 g. Post-mortem examination of turkey carcasses showed that the indicators of the experimental and control groups differ. In terms of external organoleptic characteristics, the meat obtained from the slaughter of experimental birds does not differ from the meat of poultry in the control group. Conclusions and prospects for further research. The use of probiotics based on lactic acid bacteria proved to be the most effective and ensured the complete preservation of livestock. Due to competition, it reduced the level of coliform bacteria by an average of 6.67 % in the experimental groups; the level of lactic acid bacteria in the experimental groups was 4.89 % higher than in the control. The use of probiotics increased the weight gain of poultry by 3.28 %. No significant effect on the organoleptic and physicochemical properties of meat was observed.In the future it is planned to study the microflora of turkey slaughter products.

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The use of probiotics in the cultivation of turkeys

Kytaieva

Петров

2020

SMLNUVMB

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“…The issue of obtaining clean ecological poultry products in Ukraine is extremely relevant (Dyshlyuk et al, 2017;Chukhrai, 2019). The poultry industry of Ukraine has taken a course towards the development of organic poultry farming, the purpose of which is the production of organic products, that is, highquality and safe raw materials and goods (Tochylyna et al, 2014;Romanovych, 2018;Hudzenko et al, 2019).…”

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confidence: 99%

Antagonistic properties of a probiotic preparation containing bacteria of the genera Bacillus and Enterococcus

Chechet

Kovalenko

Horbatyuk

et al. 2022

Regul. Mech. Biosyst.

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Complex probiotic preparations with spore-forming microorganisms of the genus Bacillus and other types of bacteria in their composition have a high biological activity and are of scientific and practical interest. In our laboratory experiments, we determined the level of antagonistic activity of the newly developed probiotic preparation containing strains of bacteria B. subtilis, B. licheniformis, B. coagulans in a complex with Enterococcus faecium, products of their own fermentation, other natural substances and a sorbent made of natural aluminosilicates. The antagonistic properties of the probiotic preparation were examined by diffusion methods of delayed antagonism and agar blocks. In order to identify the antagonistic action of the experimental probiotic preparation, we used Gram-negative and Gram-positive test cultures of bacteria from the Museum of Strains of Microorganism Cultures of the Research Bacteriology Department. The results of laboratory studies indicated the antagonistic effectiveness of the experimental probiotic preparation in its interaction with test microorganisms. This was confirmed by the indicators of the diameters of the growth inhibition zones of the tested bacteria, ranging 27–36 and more and corresponding to high and very high levels of antagonistic activity of the experimental probiotic. The use of the developed probiotic preparation in poultry farming would ensure the correct formation of microbiocenosis in gthe astrointestinal tract of poultry from hatching, increase local immunity, support a stable epizootic situation by preventing bacterial infections among poultry through biological means, and therefore contribute to production of ecologically high-quality and safe products of poultry farming.

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“…In industrial poultry farming conditions, especially when organic production technologies are used, the most feasible approach is to use animal protection means based on natural substances with a high suppressive effect against infectious agents and the ability to balance the immune response. Today, the Ukrainian market can afford probiotics, which include elements of commensal microbiota (lacto-and bifidobacteria) with antibacterial and immunomodulatory properties (Milian et al, 2014;Pavlova, 2015;Pitino et al, 2010;Ashraf and Shah, 2014;Romanovych, 2018).…”

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Toxicological and Biochemical Parameters of the Influence of the New Probiotic for Pigs Based on Bacillus Bacteria on Laboratory and Target Species of Animals

Buchkovska,

Chechet,

Romanko

et al. 2023

OneHealthJournal

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The development of new domestic probiotics targeted at immunomodulatory effects is both timely and holds scientific and practical value. To carry out preclinical studies of the 'Combio' probiotic, which is a mixture of probiotic bacteria Bacillus subtilis, Bacillus licheniformis, Bacillus amyloliquefaciens, Enterococcus faecium and other substances developed by the team of authors of SSRILDVSE, under the conditions of several toxicological experiments on laboratory (white rats, rabbits) and target animals toxico-biochemical parameters were identified. The findings of the trial of the acute toxicity of the 'Combio' probiotic showed that the LD50 value could not be calculated, since the death of laboratory animals was not registered within 14 days after administration; the maximum administered dose of the preparation (by absolute weight) was 30,000.0 mg/kg of body weight, which allows it to be assigned to toxicity class VI – relatively harmless substances (LD50>15,000.0 mg/kg of body weight), and according to the degree of danger to IV class – low-risk substances (LD50>5000.0 mg/kg body weight). When applied to the skin (acute dermal toxicity) and the mucous membrane of the eye of rabbits in doses from 750.0 to 3000.0 mg/kg of body weight, the 'Combio' probiotic did not have an irritating effect, and according to the degree of danger, it can be classified as IV class – low- hazardous substances (LD50>2500.0 mg/kg of body weight). According to the results of extended oral feeding of the probiotic in doses of 1500.0; 7500.0 and 15000.0 mg/kg of feed it showed no evidence of hematological, hepatic, or nephrotoxic effects on the laboratory animals under subacute toxicological conditions. On the contrary, it showed the ability to induce metabolic responses in the bodies of white rats (according to dynamics of hematopoiesis and liver protein-synthesizing functions, especially at therapeutic doses). The dynamics of changes in metabolic indicators during extended 60-day oral feeding of probiotics to target animals and poultry in the dose range showed no evidence of hematological, immunological, or hepatoxic effects and correlated with the hematological indicators, which indicated the restoration of nonspecific resistance and the protein profile in the bodies of experimental animals and a sign of increased metabolic respond and immune responsiveness in pigs organisms. The findings regarding the effect of the new 'Combio' probiotic based on the bacteria Bacillus spp. in several experiments on laboratory and target (pigs) animals give grounds to assert that it is ecologically safe and promising for the result – improving the survival rate of target animals and poultry, increasing their body weight gain, optimizing feed conversion, and enhancing the overall quality of production.

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The dynamics of humoral protection factors in broilers under the conditions of probiotic preparations application

Cited by 4 publications

References 3 publications

The use of probiotics in the cultivation of turkeys

The use of probiotics in the cultivation of turkeys

Antagonistic properties of a probiotic preparation containing bacteria of the genera Bacillus and Enterococcus

Toxicological and Biochemical Parameters of the Influence of the New Probiotic for Pigs Based on Bacillus Bacteria on Laboratory and Target Species of Animals

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