The Early Adhesion Effects of Human Gingival Fibroblasts on Bovine Serum Albumin Loaded Hydrogenated Titanium Nanotube Surface

Abstract

The soft tissue sealing at the transmucal portion of implants is vital for the long-term stability of implants. Hydrogenated titanium nanotubes (H2-TNTs) as implant surface treatments were proved to promote the adhesion of human gingival fibroblasts (HGFs) and have broad usage as drug delivery systems. Bovine serum albumin (BSA) as the most abundant albumin in body fluid was crucial for cell adhesion and was demonstrated as a normal loading protein. As the first protein arriving on the surface of the implant, albumin plays an important role in initial adhesion of soft tissue cells, it is also a common carrier, transferring and loading different endogenous and exogenous substances, ions, drugs, and other small molecules. The aim of the present work was to investigate whether BSA-loaded H2-TNTs could promote the early adhesion of HGFs; H2-TNTs were obtained by hydrogenated anodized titanium dioxide nanotubes (TNTs) in thermal treatment, and BSA was loaded in the nanotubes by vacuum drying; our results showed that the superhydrophilicity of H2-TNTs is conducive to the loading of BSA. In both hydrogenated titanium nanotubes and non-hydrogenated titanium nanotubes, a high rate of release was observed over the first hour, followed by a period of slow and sustained release; however, BSA-loading inhibits the early adhesion of human gingival fibroblasts, and H2-TNTs has the best promoting effect on cell adhesion. With the release of BSA after 4 h, the inhibitory effect of BSA on cell adhesion was weakened.

Facile Fabrication of Transparent and Opaque Albumin Methacryloyl Gels with Highly Improved Mechanical Properties and Controlled Pore Structures

“…It is one component of the tissue-culture media, and a scaffold based on albumin should work well for cell and tissue engineering because it can provide structural support [ 6 , 7 , 8 ]. It has also been suggested as a biomaterial scaffold for in-vivo implants [ 9 ] and in-vitro culture of fibroblasts, osteoblasts, and other cells [ 10 , 11 , 12 , 13 ]. With good cell viability, photocurable albumin hydrogels demonstrated tunable physiological characteristics over swelling, mechanical properties, and degradation [ 14 , 15 , 16 ].…”

Effect of Ionic and Non-Ionic Surfactant on Bovine Serum Albumin Encapsulation and Biological Properties of Emulsion-Electrospun Fibers

“…Proteins and peptides are of significant interest among them, not only due to their impact on tissue regeneration (as they are a basic component of the cells) but also for their role in enzymatic catalysis, metabolic processes, signal transduction, and immune response. Human serum albumin (HSA) is one of the most abundant proteins in human plasma that performs dozens of important functions, including the transport of amino acids, fatty acids, steroids, and drugs; regulation of pH and oncotic blood pressure [ 5 , 6 , 7 ]. It also removes harmful factors for the cell’s survival, such as reactive oxygen species, and protects them against lysis (in low concentrations such as 1 g/L), making it an excellent molecule for both in vitro (maintaining the eucaryotic cell cultures) and in vivo conditions.…”

“…Due to the resemblance of the structure to HSA, bovine serum albumin (BSA) is one of the most used model proteins for studying the reaction of the human organism to biomaterials. It can be used both as the ion or drug carrier as well as the modifier itself (e.g., in a sustained delivery system for promoting early adhesion of the human gingival fibroblasts) [ 5 , 6 ].…”

Preparation of honokiol-loaded titanium dioxide nanotube drug delivery system and its effect on CAL-27 cells

“…After drug loading, TNTs can play a nano role in tumor cells and improve the effect of a single drug on tumor treatment (Liao et al, 2020;Liu et al, 2022). In addition, Sun et al proved that TNTs carrier has no effect on drug stability and is non-toxic and harmless to the body itself (Hasanzadeh Kafshgari et al, 2019;Sun et al, 2021).…”