The Early Development of Articular Cartilage After Perichondrial Grafting

Ohlsén, Lennart; Widenfalk, Bertil

doi:10.3109/02844318309013114

Cited by 54 publications

(22 citation statements)

References 21 publications

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“…data] also confirmed that the pro liferation zone is the area of the most intensive prolifer ation when cultured in a chondrogcnic milieu. The same changes of cell configuration and increasing staining with toluidine blue were found as Ohlsen and Widenfalk [21] did.…”

Section: Discussionsupporting

confidence: 66%

“…Ohlsen and Widenfalk [21] found, in comparison with the original tissue in transplants, the most active zone in the innermost part of the perichondrium because these cells had extremely changed their configuration after transplan tation into cartilage defects.…”

Section: Discussionmentioning

confidence: 99%

“…Later on Ohlsen and Widenfalk [21] examined the use of fibrin glue for transplant fixation. They gave a distinct description of the rabbit's original rib perichondrium prior to transplantation into cartilage defects in the knee joint as well as a detailed histological description of the different stages of the new developing cartilage.…”

Section: Discussionmentioning

confidence: 99%

“…The specific tissue is obtainable from the lower ribs using a spe cial punch and/or raspatorium which elevates the tissue from the underlying rib cartilage. During elevation, perichondrium loosens from the underlying cartilage in a certain layer, but macroscopically it is impossible to make out a separation line between perichondrium and the rib cartilage [21 ].…”

Section: Methodsmentioning

confidence: 99%

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The Rib Perichondrium

Bruns

Meyer-Pannwitt²,

Silbermann³

1992

Cells Tissues Organs

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Perichondrial tissue is of great importance as a transplant for the repair of hyaline-cartilage defects of synovial joints because of its potential to produce hyaline-like cartilage. The histological and histochemical character and fiber orientation of rib perichondrial grafts prior to a transplantation procedure for the repair of full-thickness hyaline-cartilage defects is analyzed in a sheep model. Compared to other descriptions of the rib perichondrium of other different species and humans, the perichondrial architecture of different origins demonstrates the same histological, histochemical character and fiber orientation. The three different layers of this tissue are: the outer fibrous layer, the central part called proliferation zone and the inner part towards the underlying rib called transition zone. The fibrous layer demonstrates a wave-like extracellular configuration and contains a few fibrocytes. The proliferation zone is only a few cell rows thick and contains single cells with an oval shape and longitudinal fibrocyte-like nucleus. Because of the potential of these cells to produce cartilage this layer is called proliferation or cambium layer. The transition zone contains more oval-shaped cells with rounded nuclei and demonstrates a more chondrocytic character. Some cells lie in groups of 2 and more cells like in chondrons. The extracellular matrix shows an increasing stain towards the rib cartilage indicating new cartilage production. The fiber orientation is longitudinal in the fibrous part and vertical in the transition zone. Between these layers, fibers deviate from the longitudinal to the vertical orientation corresponding to the light-microscopically visible proliferation zone.

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Section: Discussionsupporting

confidence: 66%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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The Rib Perichondrium

Bruns

Meyer-Pannwitt²,

Silbermann³

1992

Cells Tissues Organs

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“…Mesenchymal cells also differentiate into fibroblasts, and these cells form the perichondrium covering the cartilage. Regarding the perichondrial structure, 1-to 3-layer structures have been reported [Engkvist, 1979;Engkvist et al, 1979;Ohlsén and Widenfalk, 1983]. Chondrogenic cells with an ability to differentiate into chondrocytes are present in the perichondrium.…”

Section: Discussionmentioning

confidence: 99%

Effect of Local Environment, Fibrin, and Basic Fibroblast Growth Factor Incorporation on a Canine Autologous Model of Bioengineered Cartilage Tissue

Morotomi¹,

Wada²,

Uehara³

et al. 2012

Cells Tissues Organs

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We developed a technique to form a bioabsorbable synthetic polymer (polyglycolic acid, PGA) combined with a natural polymer (fibrin) to serve as a scaffold to help retain seeded cells and improve the seeding efficiency of chondrocytes in an implantable construct. This approach was evaluated in a canine autologous implant model of bioengineered cartilage. The implantation site (subcutaneous or intrafascial) and the use of basic fibroblast growth factor (b-FGF) were also evaluated with this system. The intrafascial implantation site yielded optimal results, especially when used in conjunction with fibrin and a b-FGF sustained-release system incorporated into the complex. A thicker, more sustained cartilagenous layer was formed, with a more vascularized outer fibrous supporting tissue layer. This combined approach of implant environment selection, natural polymer for cell retention, and growth factor supplementation offers a more optimized method for generating bioengineered auricular cartilage.

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Neocartilage after artificial cartilage repair in the rabbit: Histology and proteoglycan fragments in joint fluid

Messner¹,

Lohmander²,

Gillquist³

1993

J. Biomed. Mater. Res.

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Repair of full-thickness cartilage defects of the rabbit medial femoral condyle with artificial plugs and periosteal grafts was followed for 1 year. The morphological characteristics of the neocartilage after repair and the concentration of proteoglycan fragments in the joint fluid (CPFF) were evaluated. The CPFF showed only a weak correlation to the histological score of the neocartilage. Normal cartilage and subchondral bone did not develop with any of the repair alternatives. With a Dacron plug, neocartilage formation appeared earlier than with natural repair and was better than with periosteal grafting. Increased CPFFs were found in all specimens with repair at 3 months, probably reflecting the remaining effect of surgery. At 6 months, the CPFFs had normalized to control values. At 1 year, the CPFFs were again similar to those at the 3-month evaluation. This was paralleled by a degeneration of the neocartilage. We conclude that Dacron plugs could serve as a scaffold for neocartilage formation. The CPFF reflected surgical trauma and late degeneration of neocartilage but did not indicate minor differences in quality between cartilage repair techniques, probably because of the limited size of the grafted defect.

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The Early Development of Articular Cartilage After Perichondrial Grafting

Cited by 54 publications

References 21 publications

The Rib Perichondrium

The Rib Perichondrium

Effect of Local Environment, Fibrin, and Basic Fibroblast Growth Factor Incorporation on a Canine Autologous Model of Bioengineered Cartilage Tissue

Neocartilage after artificial cartilage repair in the rabbit: Histology and proteoglycan fragments in joint fluid

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