The ecological dose value (ED50) for assessing Cd toxicity on ATP content and dehydrogenase and urease activities of soil

Moreno, J.L.; Garcı́a, Carlos; Landi, L.; Falchini, L.; Pietramellara, Giacomo; Nannipieri, Paolo

doi:10.1016/s0038-0717(00)00189-9

Cited by 87 publications

(46 citation statements)

References 22 publications

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“…Soil respiration, metabolic quotient, and soil enzymatic activity have been adequate indicators for microbial activity and of modifications occurred in the soil to evaluate the effects of sewage sludge disposal in agriculture and forestry soils [7,[37][38][39][40].…”

Section: Effect Of Sewage Sludge On Soil Microbial Activitymentioning

confidence: 99%

Impacts of Sewage Sludge in Tropical Soil: A Case Study in Brazil

Bettiol

Ghini

2011

Applied and Environmental Soil Science

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A long-term assay was conducted to evaluate the environmental impacts of agriculture use of sewage sludge on a tropical soil. This paper describes and discusses the results obtained by applying a interdisciplinary approach and the valuable insights gained. Experimental site was located in Jaguariúna (SP, Brazil). Multiyear comparison was developed with the application of sewage sludge obtained from wastewater treatment plants at Barueri (domestic and industrial sewage) and Franca (domestic sewage), São Paulo State. The treatments were control, mineral fertilization, and sewage sludge applied based on the N concentration that provides the same amount of N as in the mineral fertilization recommended for corn crop, two, four, and eight times the N recommended dosage. The results obtained indicated that the amount of sewage sludge used in agricultural areas must be calculated based on the N crop needs, and annual application must be avoided to prevent overapplications.

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Section: Effect Of Sewage Sludge On Soil Microbial Activitymentioning

confidence: 99%

Impacts of Sewage Sludge in Tropical Soil: A Case Study in Brazil

Bettiol

Ghini

2011

Applied and Environmental Soil Science

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“…Major goals of these efforts are to attribute key functions to specific community members and, in view of the ecosystem stability, to reveal cooperation between community members and functional redundancies. Besides measuring enzyme activities, respiration rates and metabolite concentrations (Landi et al, 2000;Moreno et al, 2001), nucleic acids (Eilers et al, 2000) and lipids (Fredrickson et al, 1995) are often used as markers for microbial identity, and to a certain extent, metabolic potential and its expression in environmental samples (Kanagawa, 2003;Fields et al, 2006). Structural data obtained from lipids are based on the occurrence of fatty acids or quinones that are specific for certain taxa.…”

Section: Introductionmentioning

confidence: 99%

Functional metaproteome analysis of protein extracts from contaminated soil and groundwater

et al. 2007

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Using proteins from soil or groundwater as functional biomarkers requires efficient extraction. We developed an extraction method in which the separation of proteins from the inorganic and organic constituents of the soil matrix was achieved by a combination of 0.1 m NaOH treatment and phenol extraction. Incubation with NaOH released humic acids and proteins from soil minerals, and simultaneously, disrupted microorganisms. The subsequent phenol extraction separated the proteins from the humic organic matter. Protein extracts were applied to sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and 2D-electrophoresis (2-DE). Spots and bands were excised and individual proteins identified by liquid chromatography online linked to mass spectrometry (MS) via electrospray ionization source (LC-ESI-MS). To assess the suitability of the method for the functional analysis of environmental metaproteomes, it was applied to soil that had been enriched in chlorophenoxy acid-degrading bacteria by incubation with 2,4-dichlorophenoxy acetic acid (2,4-D) for 22 days. The method was also used to analyze groundwater from the aquifer of a chlorobenzene-contaminated site. The identification of enzymes such as chlorocatechol dioxygenases was consistent with bacterial metabolic pathways expected to be expressed in these samples. The protocol enabled thus the analysis of the metaproteome of soil and groundwater samples. It thereby provides a means to study the diversity of environmental microbial communities while addressing functional aspects more directly than metagenome or even metatranscriptome analysis.

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“…On the one hand, the introduction of glucose stimulates the development of organisms, changing dormant forms into active forms; on the other hand, the toxic metal inhibits processes until the death and elimination of the separate groups of microorganisms. The negative effect of metals and cadmium, in particular, has been described in publications (Akmal et al, 2005;Bérarda et al, 2014;Moreno et al, 2001;Park et al, 2011;Renella et al, 2005;Vig et al, 2003). Thus, changes in growth characteristics of microbial communities isolated from soil samples fed with cadmium or glucose may indicate a change in the original community.…”

Section: Discussionmentioning

confidence: 98%

“…Both processes are influenced by environmental factors, such as the availability of nutrients and the presence of toxicants. The most commonly used parameters enabling to characterize the intensity of the anabolic and catabolic processes carried out by microorganisms include microbial biomass, fixation of atmospheric nitrogen, respiratory activity, soil enzymatic activity, the number of individual groups of microorganisms (Brookes, 1995;Chander and Brookes, 1991;1992;Moreno et al, 2001;Renella et al, 2005;Tejada et al, 2011;Vig et al, 2003). When considering such metal as cadmium, it is noted that this metal either alone or in combination with other metals causes a change in the microbial community parameters, at that the authors suggest a wide range of concentrations causing adverse effect-from 0.1-1 to 4.000 mg kg −1 (Vig et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Influence of Cadmium and Glucose on Soil Microbial Communities

Gilmullina¹,

Galitskaya²,

Selivanovskaya³

2015

American Journal of Applied Sciences

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A laboratory-based simulation of exposure of the soil community in a gray forest soil to cadmium and glucose has been conducted. It was established that while growing in a fertile medium microbial communities isolated from a soil sample contaminated with cadmium have a substantially increased phase of growth delay and there is a reduction in total growth. While analyzing communities by Biolog Ecoplate method, it was found that, as compared to the community isolated from a soil rich in glucose, the community in a soil with cadmium is characterized by significantly lower AWCD, R and H indices, in which the differences increase with time of incubation. Also differences in the pattern and rate of recyclable substrates have been revealed. The resulting data suggest changes in the functional activity of the microbial community in the soil under the influence of cadmium.

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The ecological dose value (ED50) for assessing Cd toxicity on ATP content and dehydrogenase and urease activities of soil

Cited by 87 publications

References 22 publications

Impacts of Sewage Sludge in Tropical Soil: A Case Study in Brazil

Impacts of Sewage Sludge in Tropical Soil: A Case Study in Brazil

Functional metaproteome analysis of protein extracts from contaminated soil and groundwater

Influence of Cadmium and Glucose on Soil Microbial Communities

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