We measured seasonal effects of wastewater treatment plant (WTP) effluent on growth, survival, and accumulation of microbes in oysters near a major WTP in Mobile Bay, AL. Despite higher nutrients near the WTP, seasonal conditions rather than distance affected chlorophyll a concentration and oyster growth. In summer and fall, when oyster growth was higher, δ 15 N‰ in oysters near the WTP changed through time to reflect δ 15 N‰ in effluent (approx. −4‰). Microbial indicators (male-specific coliphage, fecal coliforms) were highest in oysters near the WTP in all seasons and correlated with δ 15 N‰ in fall and summer. Increased riverine discharge and slower acquisition of δ 15 N‰ likely confounded correlations in winter/spring. Although we did not detect gross ecological effects of wastewater exposure for oysters, data indicated wastewaterderived particles entered the local food web and accumulated in oysters. These data highlight the importance of using multiple indicators of wastewater exposure and considering both seasonal and spatial effects when defining wastewater influence on a system or species.