The ECSIM Concept (Environmental Control System for Intestinal Microbiota) and Its Derivative Versions to Help Better Understand Human Gut Biology

Brugère, Jean-François; Féria-Gervasio, David; Popse, Zsolt; Tottey, William; Alric, Monique

doi:10.5772/21602

Cited by 5 publications

(3 citation statements)

References 46 publications

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“…pH, temperature, retention time, nutrient availability, and anoxic conditions). Existing dynamic (semi-)continuous in vitro models have been mainly developed and validated to study the colon microbial community {TIM2® [TNO gastro-Intestinal tract Model (Stolaki et al 2019 )], ARCOL [Artificial Colon (Deschamps et al 2020 )], ECSIM [Environmental Control System for Intestinal Microbiota (Brugère et al 2011 )], and SHIME® [Simulator of the Human Intestinal Microbial Ecosystem (van de Wiele et al 2015 )]}, though by the increasing evidence of the importance of the small intestinal microbiota to human health (Aidy et al 2015 ), some of these were adapted to study the small intestinal ecology.…”

Section: Models Of the Small Intestinal Environmentmentioning

confidence: 99%

The small intestine: dining table of host–microbiota meetings

Delbaere

Roegiers

Bron

et al. 2023

FEMS Microbiology Reviews

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Growing evidence suggests the importance of the small intestinal bacteria in the diet-host-microbiota dialogue in various facets of health and disease. Yet, this body site is still poorly explored and its ecology and mechanisms of interaction with the host are just starting to be unraveled. In this review, we describe the current knowledge on the small intestinal ecology, its composition and diversity, and how the intestinal bacteria in homeostatic conditions participate in nutrient digestion and absorption. We illustrate the importance of a controlled bacterial density and of the preservation of absorptive surface for the host's nutritional status. In particular, we discuss these aspects of the small intestinal environment in the framework of two disease conditions, namely small intestinal bacterial overgrowth (SIBO) and short bowel syndrome (SBS). We also detail in vivo, ex vivo and in vitro models developed to simulate the small intestinal environment, some applied for (diet-)host-bacteria interaction studies. Lastly, we highlight recent technological, medical and scientific advances applicable to investigate this complex and yet understudied body environment to broaden our knowledge in support of further progress in the medical practice, and to proceed towards the integration of the (small)intestinal bacteria in personalized therapeutic approaches.

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Section: Models Of the Small Intestinal Environmentmentioning

confidence: 99%

The small intestine: dining table of host–microbiota meetings

Delbaere

Roegiers

Bron

et al. 2023

FEMS Microbiology Reviews

View full text Add to dashboard Cite

show abstract

“…Modifications to ECSIM also include combining reactors to simulate all parts of the intestine-3S-ECSIM (three stage ECSIM). This study is, however, a long process because it requires stabilization at different stages, which can last up to 10 days [81]. The ECSIM model is not frequently used in scientific research.…”

Section: Proximal Environmental Control System For Intestinal Microbi...mentioning

confidence: 99%

Artificial Gastrointestinal Models for Nutraceuticals Research—Achievements and Challenges: A Practical Review

et al. 2022

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Imitating the human digestive system as closely as possible is the goal of modern science. The main reason is to find an alternative to expensive, risky and time-consuming clinical trials. Of particular interest are models that simulate the gut microbiome. This paper aims to characterize the human gut microbiome, highlight the importance of its contribution to disease, and present in vitro models that allow studying the microbiome outside the human body but under near-natural conditions. A review of studies using models SHIME, SIMGI, TIM-2, ECSIM, EnteroMix, and PolyfermS will provide an overview of the options available and the choice of a model that suits the researcher’s expectations with advantages and disadvantages.

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“…TIM focusses on the meal transit time, and it controls physiological parameters to reflect the conditions of the upper gastrointestine; thus, experiments have a short timeframe (>72 h). Similarly, models such as EnteroMix [ 31 ] and ECSIM [ 32 ] also have reduced run times when compared to the triple-stage model and SHIME. As these models aim at investigating the digestion process, they do not offer the experimental durability of weeks/months that CDI studies often require.…”

Section: Current In Vitro Models For Studying ...mentioning

confidence: 99%

In vitro models to study Clostridioides difficile infection: current systems and future advances

Ewin

Birch

Moura

2022

Current Opinion in Gastroenterology

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Purpose of reviewClostridioides difficile infection (CDI) is the most common cause of healthcare-associated diarrhoea in western countries, being categorized as an urgent healthcare threat. Historically, researchers have relied on the use of in vivo animal models to study CDI pathogenesis; however, differences in physiology and disease prognosis compared with humans limit their suitability to model CDI. In vitro models are increasingly being used as an alternative as they offer excellent process control, and some are able to use human ex-vivo prokaryotic and/or eukaryotic cells. Recent findingsSimulating the colonic environment in vitro is particularly challenging. Bacterial fermentation models have been used to evaluate novel therapeutics, explore the re-modelling of the gut microbiota, and simulate disease progression. However, they lack the scalability to become more widespread. Models that co-culture human and bacterial cells are of particular interest, but the different conditions required by each cell type make these models challenging to run. Recent advancements in model design have allowed for longer culture times with more representative bacterial populations.

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The ECSIM Concept (Environmental Control System for Intestinal Microbiota) and Its Derivative Versions to Help Better Understand Human Gut Biology

Cited by 5 publications

References 46 publications

The small intestine: dining table of host–microbiota meetings

The small intestine: dining table of host–microbiota meetings

Artificial Gastrointestinal Models for Nutraceuticals Research—Achievements and Challenges: A Practical Review

In vitro models to study Clostridioides difficile infection: current systems and future advances

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