The effect of 50 hz magnetic field of different shape on oxygen metabolism in blood platelets: in vitro studies

Abstract

Objectives: The aim of the study was to assess the influence that the shape of low frequency magnetic field may have on catalase and superoxide dismutase activity, malondialdehyde concentration and free radicals generation in human blood platelets. Materials and methods: The suspension of human blood platelets was exposed for 15 min to 50 Hz magnetic field of different shape, and flux density of 10 mT. Results: The determinations of free radicals, malondialdehyde and catalase showed increased values compared with the initial level, regardless of the shape of the magnetic field applied. In contrast, superoxide dismutase activity was lower than at the onset of the experiment. Conclusions: The findings indicate that the oxidative stress resulting from exposure to 50 Hz magnetic field of 10 mT induction may produce a number of adverse effects within the cell and thus may lead to systemic disturbances in the human body.

Does the magnetic field of a magnetic stirrer in an optical aggregometer affect concurrent platelet aggregation?

“…They reported that a 1 min exposure to the electrical field produced by a pacemaker generating pulses with amplitude of 10 V and pulse width of 1 ms at a frequency of 1.7 Hz significantly increased ADP‐induced aggregation of platelets. Henrykowska et al [2009] showed that a 15 min exposure to a 10 mT magnetic field at a frequency of 50 Hz increased the oxidative stress in platelets. Working on prostaglandin I 2 (PGI 2 ) and thromboxane A 2 (TxA 2 ) secretion in endothelial cells of the human umbilical cord vein, Caprani et al [2008] reported that a 20 µT, 1 Hz electromagnetic field exposure affected the thromboembolic equilibrium.…”

Effect of 50-Hz Sinusoidal Magnetic Field on the Production of Superoxide Anion and the Expression of Heat-shock Protein 70 in RAW264 Cells

“…This has been supported by a number of studies, eg., an increase in the O 2 -in cell line immune cells or in primary immune cells (Simkó et al, 2001;Rollwitz et al, 2004;Lupke et al, 2004;Frahm et al, 2006Frahm et al, , 2010Mannerling et al, 2010;Poniedzialek et al, 2013). Other studies have shown that the ELF-MF enhanced the exogenous oxidative stress by, for example, inactivation of hexokinase and depletion of reduced glutathione in red blood cells (Fiorani et al, 1997), or increased production of reactive oxigen species in blood platelets (Henrykowska et al, 2009). In one study brain cells were isolated from rats that had been exposed to 0.01-mT, 60-Hz MF for 24 h or 48 h (Lai and Singh, 2004).…”