The Effect of a Dodecahedron-Shaped Structure on the Properties of an Enzyme

Ivanov, Yuri D.; Tatur, Vadim Yu.; Shumov, Ivan D.; Kozlov, Andrey F.; Valueva, Anastasia A.; Ivanova, Irina A.; Ershova, Maria O.; Ivanova, Nina D.; Stepanov, Igor N.; Lukyanitsa, Andrei A.; Ziborov, Vadim S.

doi:10.3390/jfb13040166

Cited by 3 publications

(2 citation statements)

References 54 publications

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“…The absorbance of 1 nM HRP solution (containing 0.3 mM ABTS and 2.5 mM H 2 O 2 ) at 405 nm was monitored for 5 min, and the resulting dependencies were plotted in the form of absorbance vs. time ( A 405 (t) ) curves. The enzymatic activity was estimated based on the first 120 s of observation, and the calculations were performed as described previously [ 56 ].…”

Section: Methodsmentioning

confidence: 99%

Stopped Flow of Glycerol Induces the Enhancement of Adsorption and Aggregation of HRP on Mica

et al. 2023

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Glycerol is a usable component of heat-transfer fluids, and is thus suitable for the use in microchannel-based heat exchangers in biosensors and microelectronic devices. The flow of a fluid can lead to the generation of electromagnetic fields, which can affect enzymes. Herein, by means of atomic force microscopy (AFM) and spectrophotometry, a long-term effect of stopped flow of glycerol through a coiled heat exchanger on horseradish peroxidase (HRP) has been revealed. Samples of buffered HRP solution were incubated near either the inlet or the outlet sections of the heat exchanger after stopping the flow. It has been found that both the enzyme aggregation state and the number of mica-adsorbed HRP particles increase after such an incubation for 40 min. Moreover, the enzymatic activity of the enzyme incubated near the inlet section has been found to increase in comparison with that of the control sample, while the activity of the enzyme incubated near the outlet section remained unaffected. Our results can find application in the development of biosensors and bioreactors, in which flow-based heat exchangers are employed.

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Section: Methodsmentioning

confidence: 99%

Stopped Flow of Glycerol Induces the Enhancement of Adsorption and Aggregation of HRP on Mica

et al. 2023

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“…The results were presented in the form of A 405 (t) curves. The enzymatic activity was calculated based on the first 200 s of measurement as described previously [59].…”

Section: Spectrophotometric Determination Of Hrp Enzymatic Activitymentioning

confidence: 99%

Glycerol Flow through a Shielded Coil Induces Aggregation and Activity Enhancement of Horseradish Peroxidase

et al. 2023

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Glycerol has found its applications as a heat-transfer fluid in heat exchangers, and as a component of functional liquids in biosensor analysis. Flowing non-aqueous fluids are known to be able to induce electromagnetic fields due to the triboelectric effect. These triboelectrically generated electromagnetic fields can affect biological macromolecules. Horseradish peroxidase (HRP) is widely employed as a convenient model object for studying how external electric, magnetic, and electromagnetic fields affect enzymes. Herein, we have studied whether the flow of glycerol in a ground-shielded cylindrical coil affects the HRP enzyme incubated at a 2 cm distance near the coil’s side. Atomic force microscopy (AFM) has been employed in order to study the effect of glycerol flow on HRP at the nanoscale. An increased aggregation of HRP on mica has been observed after the incubation of the enzyme near the coil. Moreover, the enzymatic activity of HRP has also been affected. The results reported that their application can be found in biotechnology, food technology and life sciences applications, considering the development of triboelectric generators, enzyme-based biosensors and bioreactors with surface-immobilized enzymes. Our work can also be of interest for scientists studying triboelectric phenomena, representing one more step toward understanding the mechanism of the indirect action of the flow of a dielectric liquid on biological macromolecules.

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The Effect of a Rotating Cone on Horseradish Peroxidase Aggregation on Mica Revealed by Atomic Force Microscopy

Ivanov

Tatur²,

Shumov

et al. 2022

Micromachines

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Our study reported herein aims to determine whether an electromagnetic field, induced triboelectrically by a metallic cone, rotating at a frequency of 167 Hz, has an effect on the properties of the horseradish peroxidase (HRP) enzyme. Atomic force microscopy (AFM) was employed to detect even the most subtle effects on single enzyme molecules. In parallel, a macroscopic method (spectrophotometry) was used to reveal whether the enzymatic activity of HRP in solution was affected. An aqueous solution of the enzyme was incubated at a distance of 2 cm from the rotating cone. The experiments were performed at various incubation times. The control experiments were performed with a non-rotating cone. The incubation of the HRP solution was found to cause the disaggregation of the enzyme. At longer incubation times, this disaggregation was found to be accompanied by the formation of higher-order aggregates; however, no change in the HRP enzymatic activity was observed. The results of our experiments could be of interest in the development of enzyme-based biosensors with rotating elements such as stirrers. Additionally, the results obtained herein are important for the correct interpretation of data obtained with such biosensors.

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The Effect of a Dodecahedron-Shaped Structure on the Properties of an Enzyme

Cited by 3 publications

References 54 publications

Stopped Flow of Glycerol Induces the Enhancement of Adsorption and Aggregation of HRP on Mica

Stopped Flow of Glycerol Induces the Enhancement of Adsorption and Aggregation of HRP on Mica

Glycerol Flow through a Shielded Coil Induces Aggregation and Activity Enhancement of Horseradish Peroxidase

The Effect of a Rotating Cone on Horseradish Peroxidase Aggregation on Mica Revealed by Atomic Force Microscopy

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