The effect of AD6 (8-monochloro-3-β-diethylamino-ethyl-4-methyl-7-ethoxycarbonylmethoxycoumarin) on washed human platelet aggregation induced by platelet activating factor (PAF) and epinephrine

Zanetti, Adriana; Zatta, A.; Prosdocimi, Marco; Dejana, Elisabetta

doi:10.1016/0014-2999(86)90565-0

Cited by 14 publications

(6 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, cloricromene also inhibits washed platelet aggregation induced by platelet-activating factor (PAF) in combination with epinephrine. Since this type of aggregation, measured in the presence of physiological calcium concentrations, is independent of the cyclooxygenase pathway, it is suggested that cloricromene inhibits platelet aggregation by a mechanism different from that of cyclooxygenase blockade (40).…”

Section: Effects On Plateletsmentioning

confidence: 99%

See 1 more Smart Citation

Cloricromene

Squadrito

Prosdocimi

Altavilla

et al. 1991

Cardiovascular Drug Reviews

Self Cite

View full text Add to dashboard Cite

Section: Effects On Plateletsmentioning

confidence: 99%

“…This effect is likely to be caused by inhibition of arachidonic acid release from the cellular membranes. Furthermore, following platelet activation, induced either spontaneously or by plateletaggregating agents, the drug blocks the release of a protein from (Y granules (25,35,39,40).…”

Section: Effects On Plateletsmentioning

confidence: 99%

Cloricromene

Squadrito

Prosdocimi

Altavilla

et al. 1991

Cardiovascular Drug Reviews

Self Cite

View full text Add to dashboard Cite

“…(a vasoconstrictor) synthesis from platelets [23], Platelets. Cloricromene inhibits platelet aggregation [24] and secretion [25], reduces thromboxane B2 formation by incubated platelets [23] and inhibits the activation of human platelets by ADP alone or in combina tion with adrenaline [26].…”

Section: Neutrophils and Endothelial Adhesionmentioning

confidence: 99%

Effects of Cloricromene on Ischemia-Reperfusion Myocardial Damage in the Rabbit

Milei¹,

Ferreira²,

Grana³

et al. 1992

Cardiology

Self Cite

View full text Add to dashboard Cite

Cloricromene, a compound with several biological activities which suggest a therapeutic role in thrombosis or ischemic disease, has been studied for its effects on the extension of myocardial damage and the production of oxygen free radicals during periods of ischemia and reperfusion. In twenty rabbits the left anterior descending coronary artery (LAD) was occluded and cloricromene (3.6 µg /kg /min; n = 10) or placebo (n = 10) were continuously infused. After 50 min the artery was reopened and after 20 min of reperfusion a biopsy was obtained from the anterior wall of the heart in the LAD area and from the posterior wall (control myocardium) prior to sacrifice. Both samples were used for chemiluminescence measurement (free-radical production) and ultrastructural studies. In the placebo group all rabbits showed ST-segment changes during ischemia and reperfusion arrhythmias, while in the cloricromene group there were transient ST elevations in 4 animals which reverted at higher infusion rates of the drug. The chemiluminescence values were 18,017 ± 1,956 and 8,583 ± 918 cpm/mg protein (p < 0.001) in the anterior and posterior walls of the left ventricle, respectively, for the placebo group, and 7,767 ± 992 and 8,333 ± 832 cpm/mg protein (NS), respectively, for the cloricromene group. The ratio between the anterior and posterior wall was 2.27 ± 0.37 for the placebo group versus 0.95 ± 0.11 for the cloricromene group (p < 0.001). In ultrastructural studies, the anterior wall in the placebo group showed irreversible myocyte injury and infarction as well as mitochondrial damage. Samples from the cloricromene-treated group showed, in general, preservation of myocyte architecture or minor signs of injury. These results illustrate clearly the protective effect of cloricromene during damage induced by ischemia and reperfusion in the rabbit.

show abstract

“…Cloricromene causes changes in the throm boxane-prostacyclin equilibrium at the level of the vascular wall [14][15][16][17] and inhibits the thrombocytic activation and aggregation in duced by various stimuli such as arachidonic acid, collagen, ADP [18,19], adrenaline, or PAF [20] or by a combination of the stimuli [21,22].…”

Section: Discussionmentioning

confidence: 99%

Effects of Cloricromene on the Levels of Endothelin and on the Microcirculatory Function in Peripheral Atherosclerotic Arteriopathies

Saitta

Bonaiuto²,

Giordano³

et al. 1996

Pharmacology

View full text Add to dashboard Cite

The effects of cloricromene on plasma endothelin-1 (ET-1) levels and on microcirculatory function in 9 patients with peripheral atherosclerotic arteriopathy (PAA) and in healthy control subjects were studied. ET-1 levels and microcirculatory function were evaluated both under basal conditions and 30, 60, and 90 min after acute administration of cloricromene (30 mg i.v.). PAA patients had significantly increased levels of ET-1 and impaired vascular parameters (studied by means of Winsor’s Index, Gosling’s Index, postischemic perfusion index and recovery time) when compared to control subjects. The acute administration of cloricromene (30 mg i.v.) did not change plasma ET-1 both in control subjects and in patients with PAA. In contrast, cloricromene produced a significant improvement in the postischemic perfusion index and in recovery time in arteriopathic patients. Control subjects and patients with PAA also underwent a cold pressor test (CPT) under basal conditions and (72 h later) 30 min after an acute intravenous administration of cloricromene (30 mg i.v.). CPT caused a higher increase in ET-1 in the patients with PAA compared to the control group, and a reduction in the vascular flow at the femoral level, while the pretreatment with cloricromene prevented both the increase in the levels of ET-1 and the reduction of the femoral vascular flow observed after the cold stimulus in patients with PAA. Our data show that cloricromene, besides ameliorating the microcirculatory function, is able to interfere with dynamic mechanisms, such as those induced by the CPT, capable of stimulating the release of ET-1 at the vascular level.

show abstract

The effect of AD6 (8-monochloro-3-β-diethylamino-ethyl-4-methyl-7-ethoxycarbonylmethoxycoumarin) on washed human platelet aggregation induced by platelet activating factor (PAF) and epinephrine

Cited by 14 publications

References 26 publications

Cloricromene

Cloricromene

Effects of Cloricromene on Ischemia-Reperfusion Myocardial Damage in the Rabbit

Effects of Cloricromene on the Levels of Endothelin and on the Microcirculatory Function in Peripheral Atherosclerotic Arteriopathies

Contact Info

Product

Resources

About