The Effect of Alcoholic Extracts of Zingiberofficinale Anti-E.Coli Isolates Isolated from Urinary Tract Infection

Abstract

The Escherichia coli isolated from UTIs, were identified with biochemical tests and vitek test.The ethanolic extracts of Zingiberofficinale(Z.officinale) were tested against E.coli by using the good agar diffusion test, the alcoholic extracts from (25 -100) mg/ml showed antimicrobial activity against tested microorganism. The diameter of inhibition zone increase at high concentrations and rang from (18-20mm) , and these results compared with antibiotics sensitivity discs were used by discs diffusion method against E.coliisolates, they were resisted to all antibiotics used in this study. It could be concluded that alcoholic extract of Z.officinalehad good antimicrobial effects, and may be able to use for treatment UTIs caused by E.coli because it not make any side effect and can be used occasionally to prevent the infect by this bacteria.

Genotyping of Escherichia Coli Isolated from Diarrhea Cases in Children under Five Years of age Using the PFGE Method

“…Potential sources of DEC in this population may therefore be human or animal fecal matter. [15][16][17][18][19][20][21][22] As for the genetic convergence between the two isolates (13 and 18), 80% were that the isolation (13), which is due to the phylogenetic group (A) and did not give a result for the serotype, this may be due to the presence of new patterns that have not been studied or because the methods of serotyping O-do not always indicate the exact pathogenicity of E. coli strains, and resistance to 5 antibiotics (Nakamura et al, 2021).Isolation (18), which is belong to the phylogenetic group (F), which owns the chuA gene and it included the serotype (O25:K11) and the percentage of genetic convergence between isolates (9,14,31) and isolates (13,18) by 88% and isolated from Al-Alawi Teaching Hospital. The fifth type, which includes each of the previously mentioned isolates (9,14,31,13,18) and isolates (2,7,20,5,26), includes the two isolates (2,7), the isolation (2) belongs to the phylogenetic group (F), which has chuA gene and belongs to the serotype (O142:K86).Isolation (7) belongs to the phylogenetic group (E), which has genes (TrpBA, arpA, ChuA), did not give a serotype, and the genetic convergence rate is 80%, both of which are transmitted through contaminated food, animals are the main reservoirs of many foodborne pathogens (Moxleyet al, 2020).The isolation (20) belongs to the phylogenetic group F, which has a gene (chuA), did not give a serotype, and resistance to 5 antibiotics, and the percentage of convergence is 88% between it and isolates (2,7) and the convergence rate between the two isolates (5,21) is 90%, as the isolation (5) belongs to the phylogenetic group (E), which included the serotype (O25:K11) and has the genes (TrpBA, arpA, ChuA, TspE4c2, yjaA), while the isolation (21) belongs...…”