In vivo study revealed that ZnO nanoparticles treatment of Streptococcus SPP contaminated injured skin showed good prognosis and good healing process include complete regeneration of the epithelial cells of the epidermis and increase of cellulartiy of the dermal content compared with untreated group.In conclusion, treatment of S. pyogenes infected skin with Zinc oxide nanoparticles concentration (2 mg/ml) limit the skin damage and localized the lesion to the incision site with good healing process.

Eight isolates of methicillin resistance Staphylococcus aureus(MRSA) (SA40,SA32,SA30,SA13,SA10,SA36,SA3 and SA7) with different resistance phenotypes to macrolides , lincosamides and streptogramins Were used to detect theexpression of msrA, msrB, and linA/linA'genesby using real time polymerase chain reaction before and after treatment with antibiotics (erythromycin , clarithromycin , clindamycin and lincomycin) calibrated with triosphosphateisomerase.There highst expression of these genes was after 18 hours. It was an induction in the expression of msrA gene in isolates (SA40,SA32,SA30 and SA13) in presence of erythromycin,however,the isolates showed reduction in expression level of this gene. Expression of msrB gene had been estimated along with the using of clarithromycin and there was induction in msrB expression in isolates (SA40,SA30,SA32,SA13,SA36,SA10and SA7) while the expression level was reduced in isolate (SA3). Isolates (SA30 and SA13) showed induction of linA/linA' expression with the using of clindamycin and lincomycin respectively.

The resistance to broad spectrum antimicrobials therapy such Pseudomonas aeruginosa, . Biofilms are major virulence factors to resist antibiotics; in this concern the need for providing new alternatives for antibiotics is becoming an urgent requirement,Now day therapies and applications based treatments felid nanoparticle. copper Oxide nanoparticles, were produced from MDR and strong biofilm producer P. aeruginosa isolate by biological method, against P. aeruginosa colonization of the biofilm and LuxSgene expression.This study was aimed to explore whether green synthesized copper nanoparticles (CuNPs) can function as an anti-biofilm agent of P. aeruginosa bacteria. The nanoparticles was synthesized from cell free extract of P. aeruginosa. Characterization of biosynthesized copper nanoparticles was carried out to determine the chemical and physical properties of the product usinging AFM, TEM ,XRD and UV visible spectrometry. The hexagonal structure was confirmed by XRD, size range was marked 13-19nm by TEM, was used to confirm the surface morphology. AFM analysis used to reveal the roughness and distribution of nanoparticles. UV–visible spectra of the synthesized nanoparticles recorded maximum peak at 300 nm. Copper nanoparticles showed remarkable biofilm inhibitory on multidrug resistant at (32 μg/ml) sub Minimum Inhibitory Concentration (MIC), CuNPs exhibited remarkable effect against P. aeruginosa .Strong biofilm producer strains were incubated with 1ml of sub MIC of CuNPs for 24 -48 hrs at 37C . The current study has proven that copper oxide nanoparticles possess the ability to act as anti-biofilm agents against P. aeruginosa. This was evidenced by their significant downregulation of LuxS gene expression, The results indicated a major down-regulation in LuxS expression after exposure to CuNPs, suggesting the antimicrobial agent against multidrug-resistant P. aeruginosa infections.

The Escherichia coli isolated from UTIs, were identified with biochemical tests and vitek test.The ethanolic extracts of Zingiberofficinale(Z.officinale) were tested against E.coli by using the good agar diffusion test, the alcoholic extracts from (25 -100) mg/ml showed antimicrobial activity against tested microorganism. The diameter of inhibition zone increase at high concentrations and rang from (18-20mm) , and these results compared with antibiotics  sensitivity discs were used by discs diffusion method against E.coliisolates, they were resisted to all antibiotics used in this study. It could be concluded that alcoholic extract of Z.officinalehad good antimicrobial effects, and may be able to use for treatment UTIs caused by E.coli because it not make any side effect and can be used occasionally to prevent the infect by this bacteria.