Growth Aspergillus niger on solid state fermentation media for produce protease. The best condition for protease production in the moisture is 1:30 and incubation at 40˚C for 8day at PH 8. Inoculation with 2.2×10 4 cell/ml of fungal media containing, different nitrogen sources the peptone the bast. Protease was precipitated from production media by 70% saturation of ammonium sulphat with 3.5 U/mg protein specific activities, the final purification in DEAE-cellulose 9.8 fold of purification and 0.188 yield.

In vivo study revealed that ZnO nanoparticles treatment of Streptococcus SPP contaminated injured skin showed good prognosis and good healing process include complete regeneration of the epithelial cells of the epidermis and increase of cellulartiy of the dermal content compared with untreated group.In conclusion, treatment of S. pyogenes infected skin with Zinc oxide nanoparticles concentration (2 mg/ml) limit the skin damage and localized the lesion to the incision site with good healing process.

This work is focused on the effects of kiwi zinc oxide (ZnO) nanoparticles that are prepared through green synthesis, on Staphylococcus aureus isolated from different cosmetic tools. Zinc acetate was utilized ions while kiwi peels extract was used as a reducing agent. The formation of zinc oxide nanoparticles (ZnO NPs) was con rmed by the change of the color from green to brown and the UVvisible spectral analysis which revealed a strong surface plasmon. In addition, transmission electron microscope, and Atomic Force Microscopy (AFM) characterization showed that ZnO NPs has a spherical shape with a mean diameter of 19.76 nm. Forty swab samples were taken from different cosmetics tools and were cultured. S. aureus was identi ed by the biochemical and molecular identi cation methods. Results showed powder sponge recorded the highest, among the cosmetic tools, that harbors staphylococci bacteria. The Minimum Inhibitory Concentration (MIC) with different concentrations (128, 64, 32 and 16) mg/ml of ZnO NPs, was studied. A well diffusion test was performed to detect S. aureus sensitivity towards Kiwi ZnO nanoparticles and the result showed that the lowest inhibition zone (of 17 mm) was produced by (16mg/ml) concentration and the greatest zone (of 27 mm) was recorded by (128mg/ml) concentration.

No abstract

This work is focused on the effects of kiwi zinc oxide (ZnO) nanoparticles that are prepared through green synthesis, on Staphylococcus aureus isolated from different cosmetic tools. Zinc acetate was utilized ions while kiwi peels extract was used as a reducing agent. The formation of zinc oxide nanoparticles (ZnO NPs) was confirmed by the change of the color from green to brown and the UV-visible spectral analysis which revealed a strong surface plasmon. In addition, transmission electron microscope, and Atomic Force Microscopy (AFM) characterization showed that ZnO NPs has a spherical shape with a mean diameter of 19.76 nm. Forty swab samples were taken from different cosmetics tools and were cultured. S. aureus was identified by the biochemical and molecular identification methods. Results showed powder sponge recorded the highest, among the cosmetic tools, that harbors staphylococci bacteria. The Minimum Inhibitory Concentration (MIC) with different concentrations (128, 64, 32 and 16) mg/ml of ZnO NPs, was studied. A well diffusion test was performed to detect S. aureus sensitivity towards Kiwi ZnO nanoparticles and the result showed that the lowest inhibition zone (of 17 mm) was produced by (16mg/ml) concentration and the greatest zone (of 27 mm) was recorded by (128mg/ml) concentration.