Evaluation the Antimicrobial Action of Kiwifruit Zinc Oxide Nanoparticles Against Staphylococcus aureus Isolated from Cosmetics Tools

Abstract

This work is focused on the effects of kiwi zinc oxide (ZnO) nanoparticles that are prepared through green synthesis, on Staphylococcus aureus isolated from different cosmetic tools. Zinc acetate was utilized ions while kiwi peels extract was used as a reducing agent. The formation of zinc oxide nanoparticles (ZnO NPs) was con rmed by the change of the color from green to brown and the UVvisible spectral analysis which revealed a strong surface plasmon. In addition, transmission electron microscope, and Atomic Force Microscopy (AFM) characterization showed that ZnO NPs has a spherical shape with a mean diameter of 19.76 nm. Forty swab samples were taken from different cosmetics tools and were cultured. S. aureus was identi ed by the biochemical and molecular identi cation methods. Results showed powder sponge recorded the highest, among the cosmetic tools, that harbors staphylococci bacteria. The Minimum Inhibitory Concentration (MIC) with different concentrations (128, 64, 32 and 16) mg/ml of ZnO NPs, was studied. A well diffusion test was performed to detect S. aureus sensitivity towards Kiwi ZnO nanoparticles and the result showed that the lowest inhibition zone (of 17 mm) was produced by (16mg/ml) concentration and the greatest zone (of 27 mm) was recorded by (128mg/ml) concentration.

Antibacterial and therapeutic effects of Vancomycin-resistant Staphylococcus aureus bacteriocin (VRSAcin) in treatment of VRSA skin infection in mice

“…Moreover, the activity of stain bacteriocins increased constantly during the exponential phase of growth, and the highest activity was reached by the end of this phase. It was observed from the experiment that maximum production has been achieved at 37 °C with an inhibition zone of 18mm in diameter against tested bacteria, while the bacteriocin product at 40°C gave a 13 mm inhibition zone, while other temperatures (20,25,30) showed a minimum inhibition zone of 8, 10, and 12 mm, respectively.…”

“…VRSAcin solution Samples were exposed to a range of temperatures (20,25,30,37, and 40 °C) for 30 minutes. Protein concentrations of all samples were measured prior to and after the heat testing [19].…”

“…The results of the antibiotic susceptibility test coincided with those of [27], who reported that S. aureus was resistant to Ciprofloxacin (54.2%) and Gentamicin (12.5%). According to [28], 37% and 29% are resistant to Ciprofloxacin and Gentamycin, respectively, while according to [29] and [30], VRE clones, or vancomycin-resistant enterococci, are becoming a global public health concern. At this time, the only treatment available for Enterococcus-related infections that are resistant to ampicillin and aminoglycoside antibiotics is glycopeptide antibiotics.…”

Синергічний вплив гентаміцину та наночасток оксиду заліза на ген phzM синьогнійної палички

“…Th e TEM images of nanoparticles at diff erent magnifi cations are in close agreement with the results. Additionally, they correlate with (Seddiq et al, 2023). However, other fi ndings demonstrated the use of magnetic nanoparticles in synthesis through minute variations in shape and a wider size diversity, the sizes of agglomerates of the IONps, which reached a diameter of 8-33 nm (Roca et al, 2019).…”