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This work is focused on the effects of kiwi zinc oxide (ZnO) nanoparticles that are prepared through green synthesis, on Staphylococcus aureus isolated from different cosmetic tools. Zinc acetate was utilized ions while kiwi peels extract was used as a reducing agent. The formation of zinc oxide nanoparticles (ZnO NPs) was con rmed by the change of the color from green to brown and the UVvisible spectral analysis which revealed a strong surface plasmon. In addition, transmission electron microscope, and Atomic Force Microscopy (AFM) characterization showed that ZnO NPs has a spherical shape with a mean diameter of 19.76 nm. Forty swab samples were taken from different cosmetics tools and were cultured. S. aureus was identi ed by the biochemical and molecular identi cation methods. Results showed powder sponge recorded the highest, among the cosmetic tools, that harbors staphylococci bacteria. The Minimum Inhibitory Concentration (MIC) with different concentrations (128, 64, 32 and 16) mg/ml of ZnO NPs, was studied. A well diffusion test was performed to detect S. aureus sensitivity towards Kiwi ZnO nanoparticles and the result showed that the lowest inhibition zone (of 17 mm) was produced by (16mg/ml) concentration and the greatest zone (of 27 mm) was recorded by (128mg/ml) concentration.

This work is focused on the effects of kiwi zinc oxide (ZnO) nanoparticles that are prepared through green synthesis, on Staphylococcus aureus isolated from different cosmetic tools. Zinc acetate was utilized ions while kiwi peels extract was used as a reducing agent. The formation of zinc oxide nanoparticles (ZnO NPs) was confirmed by the change of the color from green to brown and the UV-visible spectral analysis which revealed a strong surface plasmon. In addition, transmission electron microscope, and Atomic Force Microscopy (AFM) characterization showed that ZnO NPs has a spherical shape with a mean diameter of 19.76 nm. Forty swab samples were taken from different cosmetics tools and were cultured. S. aureus was identified by the biochemical and molecular identification methods. Results showed powder sponge recorded the highest, among the cosmetic tools, that harbors staphylococci bacteria. The Minimum Inhibitory Concentration (MIC) with different concentrations (128, 64, 32 and 16) mg/ml of ZnO NPs, was studied. A well diffusion test was performed to detect S. aureus sensitivity towards Kiwi ZnO nanoparticles and the result showed that the lowest inhibition zone (of 17 mm) was produced by (16mg/ml) concentration and the greatest zone (of 27 mm) was recorded by (128mg/ml) concentration.

Thirty two isolates of Aspergillus fumigatus were obtained from a total of 44 samples of sputum, nose swab and tracheal aspirate from suspected patient with aspergillosis were also collected from February 2014 to June 2014.  A morphological examination of A. fumigatus was first made with naked eye and at low magnification power of microscope after that detailed examination was done by measuring the dimensions of the microscopic structures, photographing the microscopic structures and using relevant literature. Results appeared conical-shaped terminal vesicles, uniseriate row of phialides on the upper two thirds of the vesicle, conidiophore stipes were short, phialides arrange uniseriate upper vesicle conidia and parallel to axis of conidiophore, produced in chains of  spore basipetally from phialides, the chains of spore were borne directly in the absence of metulae and represented by septet and branching hyphae. The ability of A. fumigatus for GT production was investigated using thin layer Chromatography (TLC) and High Performance Liquid Chromatography (HPLC) techniques and results showed that GT was produced by 81.25% of A. fumigatus isolates. Optimum conditions for GT production by A. fumigatus 16 (AF-16) isolate were determined by submerged fermentation using Yeast extract sucrose medium. Results indicated that AF-16 isolate was the highest GT producer on Yeast Exract Sucrose medium with inoculum size 2×107 conidium and incubation at 32ºC for 15 days and the concentration of gliotoxin was (4511µg mL-1).

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