Effects of ZnO NPS on Streptococcus pyogenes in vivo

Mohammed, Luma Saeed; Ahmed, Mais E.

doi:10.36295/asro.2020.23228

Cited by 8 publications

(4 citation statements)

References 7 publications

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“…Subsequently, although the use of aseptic surgical procedures and hospital-acquired infections related to implants still result in major morbidity and mortality rate [2]. Multidrug resistance pathogenic bio lm infections causing important problem in wold new alternatives to antibiotic therapy which can effectively Noval metal nanoparticles (NPs) have gained signi cant interest in the eld of medical treatment and wide range of applications [3]. CuoNPs show antibacterial, antioxidant with cytotoxicity and different applications [4].…”

Section: Introductionmentioning

confidence: 99%

WITHDRAWN: Copper Nanoparticles on expression of LuxS of the genes in Pseudomonas aeruginosa strains anti-Biofilm formation

Ahmed¹,

Mohammed²

2023

Preprint

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The resistance to broad spectrum antimicrobials therapy such Pseudomonas aeruginosa, . Biofilms are major virulence factors to resist antibiotics; in this concern the need for providing new alternatives for antibiotics is becoming an urgent requirement,Now day therapies and applications based treatments felid nanoparticle. copper Oxide nanoparticles, were produced from MDR and strong biofilm producer P. aeruginosa isolate by biological method, against P. aeruginosa colonization of the biofilm and LuxSgene expression.This study was aimed to explore whether green synthesized copper nanoparticles (CuNPs) can function as an anti-biofilm agent of P. aeruginosa bacteria. The nanoparticles was synthesized from cell free extract of P. aeruginosa. Characterization of biosynthesized copper nanoparticles was carried out to determine the chemical and physical properties of the product usinging AFM, TEM ,XRD and UV visible spectrometry. The hexagonal structure was confirmed by XRD, size range was marked 13-19nm by TEM, was used to confirm the surface morphology. AFM analysis used to reveal the roughness and distribution of nanoparticles. UV–visible spectra of the synthesized nanoparticles recorded maximum peak at 300 nm. Copper nanoparticles showed remarkable biofilm inhibitory on multidrug resistant at (32 μg/ml) sub Minimum Inhibitory Concentration (MIC), CuNPs exhibited remarkable effect against P. aeruginosa .Strong biofilm producer strains were incubated with 1ml of sub MIC of CuNPs for 24 -48 hrs at 37C . The current study has proven that copper oxide nanoparticles possess the ability to act as anti-biofilm agents against P. aeruginosa. This was evidenced by their significant downregulation of LuxS gene expression, The results indicated a major down-regulation in LuxS expression after exposure to CuNPs, suggesting the antimicrobial agent against multidrug-resistant P. aeruginosa infections.

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Section: Introductionmentioning

confidence: 99%

WITHDRAWN: Copper Nanoparticles on expression of LuxS of the genes in Pseudomonas aeruginosa strains anti-Biofilm formation

Ahmed¹,

Mohammed²

2023

Preprint

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“…or resistant (R) to a particular drug by comparison with the standard inhibition zone (Table 1). [14,15].…”

Section: Antibiotic Resistance Testmentioning

confidence: 99%

Antibacterial and therapeutic effects of Vancomycin-resistant Staphylococcus aureus bacteriocin (VRSAcin) in treatment of VRSA skin infection in mice

qassam,

emad

2024

Preprint

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Vancomycin Staphylococcus aureus (VRSA) is a strain belonging to S. aureus that is considered the main cause of bacterial skin and soft tissue infections. It has acquired resistance to vancomycin and represents a therapeutic challenge. The current study aimed to compare the possible therapeutic effects of VRSA bacteriocin (VRSAcin) on the treatment of skin infection in mice compared with an antibiotic (linezolid). The results showed that from fifty swabs obtained from human skin wounds, only 30 samples were identified as Staphylococcus spp., and 20 samples of them were identified as VRSA strains. One isolate was selected for VRSAcin extraction depending on its antibiotic resistance using an antibiotic susceptibility test. The typical conditions for the production of VRSAcin include pH 7 and a temperature of 37°C for 48 hours. In mice, VRSA-contaminated wounds revealed severe tissue distraction and inflammation that extended to the hypodermis, while VRSA-treated skin showed mild changes and localized lesions to the epidermis and upper dermis. Linezolid-ointment-treated skin shows moderate to severe changes. In conclusion, VRSA strain infections in human burned skin were found to be more common than expected. In vivo studies in mice indicated that wounded skin infected with VRSA can be treated with VRSAcin as an antibacterial agent that promotes healing processes with obvious superiority to linezolid ointment.

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“…It has been shown that various NMs, such as polymeric or hybrid NMs, gold and magnetic nanoparticles (NPs) respond to stimuli from the external resulting in temporally regulated macromolecule release. For these reasons, synthetic NMs have been thoroughly studied and applied in pharmacology and medicine over the past 20 years, especially for diagnostic and therapeutic purposes [1]. Copper (Cu) nanoparticles are the ideal among all metal nanoparticles because of their distinctive chemical and physical characteristics, including excellent thermal conductivity, electrical conductivity and biological activity [2].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the STAT1 mRNA expression in peripheral blood cells as a predictive marker of early pregnancy in Awassi ewes

Younis,

Hatif

2024

Iraqi Journal of Science

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Staphylococcus haemolyticus is one of the most frequently isolated coagulase-negative staphylococci. The ability to form biofilm is considered as one of the most important virulence factors of coagulase negative staphylococci. There is only limited knowledge of the nature of S. haemolyticus biofilms. This study was aimed at evaluating the ability of S. haemolyticus strains to produce biofilm in the presence of copper oxide nanoparticles (CuONPs). The biological synthesis of nanoparticles is an environmentally friendly approach for large-scale production of nanoparticles. Copper oxide nanoparticles were produced in the current study from the S. haemolyticus viable cell filtrate. UV-visible (UV-Vis) spectroscopy, X-ray diffraction (XRD), atomic force microscope (AFM), field emission scanning electron microscope (FE-SEM), energy-dispersive X-ray spectroscopy (EDX) and zeta potential (ZP) analysis were used to analyze the newly synthesized CuONPs. Our findings revealed that at minimum inhibitory concentration (MIC), CuONPs showed remarkable inhibition on the biofilms produced by multidrug resistant (MDR) S. haemolyticus isolates.

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Effects of ZnO NPS on Streptococcus pyogenes in vivo

Cited by 8 publications

References 7 publications

WITHDRAWN: Copper Nanoparticles on expression of LuxS of the genes in Pseudomonas aeruginosa strains anti-Biofilm formation

WITHDRAWN: Copper Nanoparticles on expression of LuxS of the genes in Pseudomonas aeruginosa strains anti-Biofilm formation

Antibacterial and therapeutic effects of Vancomycin-resistant Staphylococcus aureus bacteriocin (VRSAcin) in treatment of VRSA skin infection in mice

Evaluation of the STAT1 mRNA expression in peripheral blood cells as a predictive marker of early pregnancy in Awassi ewes

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