The Effect of Allogenic Bone Marrow Mesenchymal Stem Cell - Platelet Rich Plasma (Bmscs - Prp) Intra-Articular Injection Effect on the Regeneration of Full-Thickness Joint Cartilage Defect on Rabbit

Abstract

Introduction: Full thickness cartilage defects are still a problem until present since the handling has not give any satisfactory results. Current handling is performed through cartilage enginering using mesenchymal stem cells alone and or in combination with growth factor. The purpose of this study was to investigate the effect of injection of intra-articular injection of Allogenic bone marrow mesenchymal stem cell -Platelet rich plasma (BMSCs-PRP) on regeneration of full thickness cartilage defect in rabbits. Method:The design of this study is a post-test only control group design using 36 New Zealand white rabbits divided into three groups treated with PRP, BMSCs and BMSCs-PRP. Results are evaluated after 10 weeks.Results: In the evaluation, macroscopic images showed the best healing in the BMSCs-PRP group. Histopathologic examination showed that in the MSCs-PRP group there was a significant increase in the amount of chondrocytes (p = 0.000), cartilage area (p = 0.000), as well as the number of Agecoprogenitor expec-tion cells (p = 0.000) and type 2 collagen (p = 0.000). Conclusions:Intra-articular injections Allogenic bone marrow mesenchymal stem cell (BMSCs-PRP) is able to regenerate and cure full-thickness joint cartilage defects through differentiation of MSCs into condroblasts.

Double membrane platelet-rich fibrin (PRF) – Synovium succeeds in regenerating cartilage defect at the knee: An experimental study on rabbit

“…Various methods have been developed for the treatment of cartilage defects ranging from bone marrow stimulation techniques with microfracture repair to restoration techniques with auto or osteochondral allograft and autologous chondrocytes implantation (ACI) [ 7 ]. Currently, the most widely developed treatment method is tissue engineering-based therapy with mesenchymal stem cells (MSCs) grafting and matrix-induced autologous chondrocytes implantation (MACI) [ 4 , 8 , 9 ]. The use of ACI and MSC tissue engineering technology has several weaknesses, namely complex facilities to develop stem cells, high cost, as well as requires twice operation and time [ 8 ].…”

“…Studies have shown that this method provides healing with fibrocartilage tissue that is histologically and biomechanically below normal hyaline cartilage [ 13 , 14 ]. Therefore, various attempts have been made to improve the outcome of these microfractures, including adding (augmenting) natural biological substances, such as intra-articular MSC, platelet-rich plasma (PRP), platelet-rich fibrin (PRF), scaffold and others as adjuvants [ 9 , 15 ]. The combination of microfractures and biologics can promote the healing to hyaline-like cartilage [ 16 ].…”