The effect of amodiaquine on oestrus cycle, ovarian histology and oxidative stress makers in regular cyclic sprague-dawley rats

Abstract

This study investigated the effect of Amodiaquine hydrochloride (AQ.HCl) administered by gavage on the oestrous cycle, ovarian morphology and on the antioxidant status of superoxide dismutase (SOD) and catalase (CAT) in the ovary of 15 regular cyclic Sprague-Dawley rats. The experiment was divided into 3 groups as follows: group I-received 6 mg/kg bw AQ.HCl for 28 day; group II-received 12 mg/kg bw of AQ.HCl for 28 days; and group III-received distilled water and served as control. The animals were autopsied on the 28 th day by cervical dislocation. Result showed that the mean length of the oestrous cycle was prolonged in all the AQ.HCl treated rats when compared to the control. The increase in mean cycle length was statistically significant (p < 0.05) in the group that received 12 mg/kg bw of AQ.HCl. Histological sections of the ovaries of the rats treated with AQ.HCl revealed that the most remarkable change was widespread follicular atresia when compared to the control. The study revealed a reduction in the antioxidant status of SOD and CAT in the ovary. This reduction in anti-oxidant status was statistically significant (p < 0.05) for CAT. The result of this study showed that AQ.HCl is deleterious to the ovary by prolonging the length of the oestrous cycle, producing widespread follicular atresia and depletion of the enzymatic antioxidant status of SOD and CAT and consequently resulting in a state of oxidative stress in the ovary.

Quinine inhibits ovulation and produces oxidative stress in the ovary of cyclic Sprague-Dawley rats

“…The report of this study is in agreement with Osinubi et al,25 who reported that quinine significantly increased levels of MDA in the testes. Also, in another study using amodiaquine, it was reported that amodiaquine caused a significant reduction in the enzymatic anti-oxidant activities of SOD and catalase in the ovaries of Sprague-Dawley rats13. An imbalance between ROS production and anti-oxidants results in oxidative stress.…”

“…Quinine was administered daily for 28 days by gavage13. At the end of the treatment period, the rats were sacrificed by cervical dislocation.…”