The Effect of Anastatica hierochuntica L. Extract on the Histology of Myometrial Cells and Prostaglandin levels (PGE2, PGF2α) in Pregnant Mice

Abstract

Anastatica hierochuntica L. is well known as a traditional medicine which brings benefit to the final trimester of pregnancy by increase uterine contractions. This study aimed to analyze the effect of A. hierochuntica L. extract on the histology of myometrial cells and prostaglandin levels (PGE2 and PGF2α) of pregnant mice. The mice were divided into three groups: (1) control group, treated by 0.5% CMC solution 10 ml/kg bw/day; (2) two treatment groups that treated by A. hierochuntica L. extract with 100 and 150 mg/kg bw/day doses, respectively. Uterine tissue was collected for histological analysis and blood serum was collected to examine the PGE2 and PGF2α levels. It obtained that myometrial hypertrophy increased significantly in the treatment groups (p = 0.001) than in the control group. Moreover, there were significant differences in the PGE2 levels (p = 0.001) and PGF2α levels (p = 0.000) between the control group and the treatment groups. The 100 mg/kg bw/day dose had a greater effect on myometrial hypertrophy than 150 mg/kg bw/day, which resulted in a higher increase of PGE2 and PGF2α levels. Hence, it was concluded that the 100 mg/kg bw/day dose of A. hierochuntica L. extract was the appropriate dose for increasing prostaglandin levels.

Phytochemical Analysis and Contractile Effects of Aqueous and Hydroethanolic Extracts of <i>Anastatica hierochuntica</i> L. (Brassicaceae) on the Isolated Uterus of Mice

“…Some researchers have hypothesized that the most crucial function of oxytocin is to increase the production of prostaglandins (potent inducers of uterine contractions) by inducing the expression of COX-2 via the activation of the pathway MAPK [3] [45]. Astutik et al demonstrated that administration of the hydroethanolic extract of Anastatica hierochutica to pregnant mice leads to increased prostaglandin levels of PGE2 and PGF2α [46]. Tannins, flavonoids, and saponins were highlighted in uterotonic plants.…”

Phytochemical Screening and Evaluation of Contractile Effects of the Aqueous and Hydroethanolic Extracts of <em>Anastatica hierochuntica</em> L. (Brassicaceae) on Pregnant Mice Isolated Myometrium

“…Some researchers have hypothesized that the most crucial function of oxytocin is to increase the production of prostaglandins (potent inducers of uterine contractions) by inducing the expression of COX-2 via the activation of the pathway MAPK [3,45]. Astutik et al demonstrated that administration of the hydroethanolic extract of Anastatica hierochutica to pregnant mice leads to increased prostaglandin levels of PGE2 and PGF2α [46]. Tannins, flavonoids, and saponins were highlighted in uterotonic plants.…”

Benefits of Fatimah grass for women's health

“…The author obtained articles from the PubMed, Scient Direct, MDPI, Google Schoolar, Research Gate, and Semantic scholar databases to find out which potential studies were appropriate and met the requirements as inclusion criteria. 25,26,27,28,and Indonesia 3,4,9,14,19,20. See Figure 1 for details on study settings. Scientific research on Fatimah Grass or Labisia Pumila was conducted to identify bioactive phytochemicals that contribute to its pharmacological properties.…”

“…Previous research has proven the effects of fatimah grass water immersion, including increasing the hormone oxytocin, antioxidant, anti-inflammatory, reducing the risk of dyslipidemia, phytoestrogenics, skin collagen synthesis, playing a role in the process of estrogen hormone therapy in menopause, improving lipid profiles, therapy for dysentery, dysmenorrhea 2,3,4,5 . Therefore, the use of Fatimah grass soaking water is still used today because it is considered to have great benefits, especially for women who are more interested in herbal or natural medicines.…”