The effect of androgenic hormones on creatinine secretion in the rat

Harvey, Alice M.; Malvin, Richard L.

doi:10.1113/jphysiol.1966.sp007954

Cited by 26 publications

(19 citation statements)

References 10 publications

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“…Even though we are aware that some error in GFR values can result from this method in very small animals, our values in GFR of the newborn rats are comparable to those measured with inulin in newborn rats by Horster and Lewy [25] (30.0 ± 2 and 44.7 ± 11 pl-min-1 *g_1 kidney weight, respec tively). In addition, overestimation of GFR due to secretion was avoided by using only female rats, which do not secrete creatinine [11]. The use of creatinine allowed us to test the effects of furosemide in the absence of the effects of anesthesia, which is usually required in order to use other markers of GFR such as inulin.…”

Section: Discussionmentioning

confidence: 99%

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Effects of Furosemide on the Renal Functions of the Unanesthetized Newborn Rat

Meléndez-Camargo¹,

Reyes²,

Meléndez³

1991

Dev Pharmacol Ther

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In neonates pharmacokinetics of furosemide is very slow, as compared to adult individuals, because it is eliminated through glomerular filtration and tubular secretion, mechanisms that are not fully developed in the newborn mammal. In addition, Henle's loop, the main site of action of this diuretic, is shorter in the neonate than in the adult animal. The aim of this study was to measure the response to furosemide in the unanesthetized newborn rat and to compare it with that of the adult. Furosemide (0.5, 1, 1.5, 2, 5 or 10 mg/kg body weight, i.p.) or vehicle were administered to newborn and adult rats and the effects on sodium, potassium and water balance were assessed. Despite the physiological characteristics of the neonatal kidney, furosemide induced a more marked sodium excretion and decrease in free water clearance in the newborn than in the adult rat. In contrast, urinary potassium losses elicited by furosemide were higher in the adult than in the newborn rat at similar doses. At both ages, the effects of the diuretic were dose-dependent. Plasma sodium decreased, whereas plasma potassium and hematocrit increased in the newborn rats, after the highest doses of furosemide. In the adult rat changes were less marked. Our results suggest that the neonatal kidney is more sensitive to equivalent doses of furosemide than the adult kidney.

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Section: Discussionmentioning

confidence: 99%

“…To avoid the error due to tubular secretion of creatinine, only female animals were used in which no secretion occurs [11]. Plasma and urinary creatinine were de termined by the Jaffe alkaline picrate method.…”

Section: Effect Of Furosemide On Glomerular Filtrationmentioning

confidence: 99%

Effects of Furosemide on the Renal Functions of the Unanesthetized Newborn Rat

Meléndez-Camargo¹,

Reyes²,

Meléndez³

1991

Dev Pharmacol Ther

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“…To avoid the error due to the tubular secretion of creatinine, only female animals, in which no secretion occurs, were used [9].…”

Section: Effect Of Indomethacin and Furosemide On Electrolyte Balancementioning

confidence: 99%

Indomethacin decreases furosemide-induced natriuresis and diuresis on the neonatal kidney

et al. 2006

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Indomethacin is used to pharmacologically occlude patent ductus arteriosus in preterm infants. It induces renal untoward effects and furosemide is administered simultaneously to counteract them. The effect of furosemide is blunted by indomethacin. We analyzed comparatively the interactions of furosemide and indomethacin at the organic anion transport system in adult and newborn individuals. Adult and 5-day-old Wistar rats were allocated into three groups: (1) indomethacin (10 mg/kg, ip); (2) furosemide (2 mg/kg, ip); and (3) indomethacin/furosemide, at the same doses. Urinary flow, glomerular filtration rate (GFR), sodium and potassium fractional excretions, and free-water and osmolal clearances were estimated. Para-aminohippuric acid (PAH) uptake was measured in renal cortical slices to study the organic anion's secretory pathway. In adult and newborn rats, furosemide-induced increments in urinary fluxes and excretions of sodium and potassium were blunted by indomethacin administered simultaneously. PAH uptake was decreased to a further extent by indomethacin than by furosemide, suggesting that inhibition of the diuretic effect might be related to competition in the secretion of furosemide. Inhibitory interaction between indomethacin and furosemide was achieved at approximately 10-fold lower concentrations in the newborn than in the adult rats, suggesting that tubular secretion in the neonate is more sensitive to the action of these drugs than in the adult individual.

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“…To avoid the error due to tubular secretion of creatinine, only female animals, in which no secretion occurs, were used (Harvey and Malvin, 1966). Plasma and urinary creatinine were determined by the Jaffe alkaline picrate method (Meléndez et al, 1991).…”

Section: Effect Of Aqueous Extract On Glomerular Filtration Ratementioning

confidence: 99%