The effect of antioxidants on the morphological picture of thymus and heart of rats intoxicated with sodium fluoride

Stawiarska-Pięta, Barbara; Grzegorzak, Natalia; Kuczera, Katarzyna; Helis, Agnieszka; Zalejska–Fiolka, Jolanta; Bielec, Beata; Birkner, Ewa; Kubina, Robert

doi:10.1016/j.toxlet.2013.05.072

Cited by 3 publications

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“…It has been demonstrated that a high intake of fluoride results in Electronic supplementary material The online version of this article (doi:10.1007/s12011-015-0458-5) contains supplementary material, which is available to authorized users. damage to T cells in the thymus of animals [3,11]. However, very little attention has focused on the underlying molecular mechanisms.…”

Section: Introductionmentioning

confidence: 99%

Modulation and the Underlying Mechanism of T Cells in Thymus of Mice by Oral Administration of Sodium Fluoride

Yin

Song

et al. 2015

Biol Trace Elem Res

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The underlying mechanism of thymic T cell regulation has been a hot topic of research in recent years. Fluoride is toxic at high concentrations and fluoride toxicity to thymic T cells was assessed in our study. To explore T cell responses to excess fluoride, different concentrations of fluoride were uptake by mice for 6 weeks. The expression of genes, including Foxn1, Cbx4, DLL4, and IL-7 gene, associated with the development and differentiation of T cells in thymic epithelial cells(TECs) was lower in the experimental groups than that in the control group. The percentages of CD4(+) and CD8(+) T cells that decreased with the fluoride administration were confirmed by flow cytometry. The mRNA levels of immunoregulatory cytokines IL-2 and IL-10, which participate in T cell proliferation, also declined in the experimental groups as compared with the control group. Expression of the T cell function-related genes CD2, PTPRC, CD69, and CD101, which are involved in thymic function in mice, decreased with the fluoride administration. Our findings suggest that the administration of high concentrations of fluoride to mice induces a decrease in CD4(+) and CD8(+) thymus T cells by harming TECs leading to the dysfunction of the thymus by altering the expression of T cell function-related genes and immunoregulatory cytokine production.

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Section: Introductionmentioning

confidence: 99%

Modulation and the Underlying Mechanism of T Cells in Thymus of Mice by Oral Administration of Sodium Fluoride

Yin

Song

et al. 2015

Biol Trace Elem Res

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“…Chronic toxicity of fluoride is more common than acute toxicity. Excessive intake of fluoride may result in skeletal and dental fluorosis in humans and animals (Irigoyencamacho, García, Mejía, & Huizar, 2015) and changes in the structure and function of soft tissues, including liver (Mukhopadhyay, Srivastava, & Chattopadhyay, 2015), brain (Zheng, Sun, Ke, Ouyang, & Zhang, 2016), lung (Shanmugam, Selvaraj, & Poomalai, 2016), spleen (Kuang et al, 2017), thymus (Stawiarska-Pięta et al, 2013), heart (Oyagbemi et al, 2017), intestine (Luo et al, 2013), and reproductive organs (Wei, Luo, Sun, Wang, & Wang, 2016). Also, the kidney is very sensitive to fluoride toxicity (Song et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Cardioprotective effects of the garlic (Allium sativum) in sodium fluoride-treated rats

Abdel-Baky

Abdel-Rahman

2020

JoBAZ

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Background: Excessive intake of fluoride may result in the development of cardiotixicity in the rats. The objective of this study was to investigate the possible cardioprotective effect of the garlic (G) on sodium fluoride (NaF)treated rats. Methods: Twenty-four male albino rats (100-120 g), 2 months old, were equally divided into control, NaF, G, and NaF + G groups. Group 1 was control group, the animals without any treatment. Group 2 was administrated with NaF orally (10 mg/kg BWT) daily. Group 3 received orally G alone (63 mg/kg BWT) daily. Group 4 was administrated with NaF + G at the same time (with the same previous doses) daily. The experimental period was for 4 weeks. Results: NaF significantly elevated the levels of serum creatinine kinase (CK), creatine kinase-MB (CK-MB), lactate dehydrogenase (LDH), aspartate aminotransferase (AST), Aalanine aminotransferase (ALT), and cardiac troponin I (cTnI). Also, there was a significant increase in the total cholesterol (TC), triglycerides (TAG), low-density lipoprotein (LDL-c) fractions, and the atherogenic effect (the mean ratios of TC/LDL-c and LDL-c/ (high-density lipoprotein) HDL-c), whereas a significant decrease in HDL-c occurred in the NaF-treated group compared with the control animals. The treatment with G+NaF ameliorated all the biochemical parameters tested. Conclusion: These results indicate that garlic has a cardioprotective effect against NaF cardiotoxicity.

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Sodium fluoride induces renal inflammatory responses by activating NF-κB signaling pathway and reducing anti-inflammatory cytokine expression in mice

et al. 2017

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Fluoride is widely distributed in the environment and often results in adverse health effects on animals and human beings. It has been proved that fluoride can induce inflammatory responses in vitro. However, very limited reports are focused on fluoride-induced inflammatory responses in vivo. In this study, mice were used to investigate sodium fluoride (NaF) induced renal inflammatory responses and the potential mechanism by using the methods of pathology, biochemistry, enzyme-linked immunosorbent assay (ELISA), quantitative real-time polymerase chain reaction (qRT-PCR) and western blot. A total of 240 ICR mice were randomly divided into four equal groups: the control group and three experimental groups (NaF was given orally at the dose of 0, 12, 24 and 48 mg/kg body weight for 42 days, respectively). The results showed that NaF in excess of 12 mg/kg induced the renal histopathological lesions, and inflammatory responses via the activation of nuclear factor-kappa B (NF-κB) signaling pathway and the reduction of anti-inflammatory cytokines expression. The activation of NF-κB signaling pathway was characterized by increasing the nitric oxide (NO) and prostaglandin E2 (PGE2) contents, inducible nitric oxide synthase (iNOS) activities and mRNA expression levels, and the mRNA and protein expression levels of cyclooxygenase-2 (COX-2), tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), interleukin-6 (IL-6) and interleukin-8 (IL-8) in three NaF-treated groups. Concurrently, the mRNA and protein expression levels of the anti-inflammatory cytokines including interleukin-4 (IL-4) and interleukin-10 (IL-10) were decreased in three experimental groups when compared with those in the control group.

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The effect of antioxidants on the morphological picture of thymus and heart of rats intoxicated with sodium fluoride

Cited by 3 publications

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Modulation and the Underlying Mechanism of T Cells in Thymus of Mice by Oral Administration of Sodium Fluoride

Modulation and the Underlying Mechanism of T Cells in Thymus of Mice by Oral Administration of Sodium Fluoride

Cardioprotective effects of the garlic (Allium sativum) in sodium fluoride-treated rats

Sodium fluoride induces renal inflammatory responses by activating NF-κB signaling pathway and reducing anti-inflammatory cytokine expression in mice

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