The effect of antioxidants on bleomycin treatment in in vitro and in vivo genotoxicity assays

Anderson, Diana; Başaran, Nurşen; Blowers, S.D.; Edwards, Anne J.

doi:10.1016/0027-5107(95)00017-d

Cited by 48 publications

(15 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Antioxidant effects of VC on oxygen radical-generated damage in human and mouse cells were suggested by Anderson et al [56]. The results obtained by the authors were similar to those shown in the present investigation.…”

Section: Discussionsupporting

confidence: 93%

Protective effects of the amino acid glutamine and of ascorbic acid against chromosomal damage induced by doxorubicin in mammalian cells

Tavares

Cecchi

Antunes

et al. 1998

Teratog. Carcinog. Mutagen.

View full text Add to dashboard Cite

Section: Discussionsupporting

confidence: 93%

Protective effects of the amino acid glutamine and of ascorbic acid against chromosomal damage induced by doxorubicin in mammalian cells

Tavares

Cecchi

Antunes

et al. 1998

Teratog. Carcinog. Mutagen.

View full text Add to dashboard Cite

“…For instance, Ohkawa et al (1988) have demonstrated in mice that selenium co-administration allows higher doses of cisplatin with reduction of nephro-and hematological toxicity, resulting in a higher therapeutic index. Therefore, nutritional status of cancer patients, especially the nutritional intake of antioxidants and their concentrations in plasma and tissues may determine the extent of oxidative organ damage by free radicals produced by cytostatic drugs (Clemens et al 1990;Hay et al 1991;Forni & Armand 1994;Meyer & Madias 1994;Anderson et al 1995;Weijl et al 1997). In our previous studies, we have shown that bilberry could be efficient against cisplatininduced ovarian and kidney damage .…”

Section: Discussionmentioning

confidence: 98%

Chemopreventive effect of bilberry (Vaccinium myrtillus) against cisplatin-induced oxidative stress and DNA damage as shown by the comet assay in peripheral blood of rats

Pandır

Kara

2014

Biologia

View full text Add to dashboard Cite

The aim of this study was to evaluate the chemopreventive effect of bilberry on cisplatin induced oxidative stress and DNA damage in rat blood. Twenty-one female Wistar-Albino rats were divided into three groups: group Iuntreated; group II -treated with cisplatin (single dose 7.5 mg/kg b.w.); and group III -treated with cisplatin (single dose 7.5 mg/kg b.w.) and bilberry (200 mg/kg b.w. for 10 days). Antioxidant enzyme systems including superoxide dismutase, catalase, glutathione peroxidase and the level of malondialdehyde (MDA) that might occur on erythrocytes have been determined and single cell gel electrophoresis (comet) was utilized to evaluate the DNA damage in lymphocytes. Treatment with cisplatin has increased the levels of MDA and decreased antioxidant enzymes in erythrocytes. Comet assay showed significantly higher values at dose of 7.5 mg/kg cisplatin as the result of oxidative DNA damage when compared to the control group. Cisplatin treatment with bilberry resulted in a highly significant (P < 0.05) decreased in the lymphocytes DNA when compared to the cisplatin group. Bilberry has been effective on antioxidant enzyme systems and MDA level and significantly decreased the comets. Our results indicate that bilberry is capable of preventing genotoxic and cytotoxic damage caused by cisplatin in peripheral blood cells in rats.

show abstract

“…The incidence of undifferentiated (mononuclear-cell) leukemias in low-dose female rats was significantly higher than that in controls. These tumors were not considered to be related to the administration of L-Ascorbic Acid because they did not occur in the female high-dose group at 84 COSMETIC INGREDIENT REVIEW Anderson et al 1995 Ascorbic Acid (1, 2, and 10 mM) Ascorbic Acid alone did not produce chromosomal aberrations BLM ( 10 µg/ml) + Ascorbic Acid (1, 2, and 10 mM)…”

Section: Carcinogenicitymentioning

confidence: 99%

“…BLM (50 mg/kg bw) Ascorbic Acid (1 g/kg bw) Ascorbic Acid reduced the % of micronucleated cells in peripheral blood but not in the bone marrow Anderson et al 1995 Bleomycin-induced chromosomal breaks in cultured peripheral blood lymphocytes…”

Section: Carcinogenicitymentioning

confidence: 99%

Final Report of the Safety Assessment of L-Ascorbic Acid, Calcium Ascorbate, Magnesium Ascorbate, Magnesium Ascorbyl Phosphate, Sodium Ascorbate, and Sodium Ascorbyl Phosphate as Used in Cosmetics1

2005

Int J Toxicol

View full text Add to dashboard Cite

L-Ascorbic Acid, Calcium Ascorbate, Magnesium Ascorbate, Magnesium Ascorbyl Phosphate, Sodium Ascorbate, and Sodium Ascorbyl Phosphate function in cosmetic formulations primarily as antioxidants. Ascorbic Acid is commonly called Vitamin C. Ascorbic Acid is used as an antioxidant and pH adjuster in a large variety of cosmetic formulations, over 3/4 of which were hair dyes and colors at concentrations between 0.3% and 0.6%. For other uses, the reported concentrations were either very low (<0.01%) or in the 5% to 10% range. Calcium Ascorbate and Magnesium Ascorbate are described as antioxidants and skin conditioning agents--miscellaneous for use in cosmetics, but are not currently used. Sodium Ascorbyl Phosphate functions as an antioxidant in cosmetic products and is used at concentrations ranging from 0.01% to 3%. Magnesium Ascorbyl Phosphate functions as an antioxidant in cosmetics and was reported being used at concentrations from 0.001% to 3%. Sodium Ascorbate also functions as an antioxidant in cosmetics at concentrations from 0.0003% to 0.3%. Related ingredients (Ascorbyl Palmitate, Ascorbyl Dipalmitate, Ascorbyl Stearate, Erythorbic Acid, and Sodium Erythorbate) have been previously reviewed by the Cosmetic Ingredient Review (CIR) Expert Panel and found "to be safe for use as cosmetic ingredients in the present practices of good use." Ascorbic Acid is a generally recognized as safe (GRAS) substance for use as a chemical preservative in foods and as a nutrient and/or dietary supplement. Calcium Ascorbate and Sodium Ascorbate are listed as GRAS substances for use as chemical preservatives. L-Ascorbic Acid is readily and reversibly oxidized to L-dehydroascorbic acid and both forms exist in equilibrium in the body. Permeation rates of Ascorbic Acid through whole and stripped mouse skin were 3.43 +/- 0.74 microg/cm(2)/h and 33.2 +/- 5.2 microg/cm(2)/h. Acute oral and parenteral studies in mice, rats, rabbits, guinea pigs, dogs, and cats demonstrated little toxicity. Ascorbic Acid and Sodium Ascorbate acted as a nitrosation inhibitor in several food and cosmetic product studies. No compound-related clinical signs or gross or microscopic pathological effects were observed in either mice, rats, or guinea pigs in short-term studies. Male guinea pigs fed a control basal diet and given up to 250 mg Ascorbic Acid orally for 20 weeks had similar hemoglobin, blood glucose, serum iron, liver iron, and liver glycogen levels compared to control values. Male and female F344/N rats and B6C3F(1) mice were fed diets containing up to 100,000 ppm Ascorbic Acid for 13 weeks with little toxicity. Chronic Ascorbic Acid feeding studies showed toxic effects at dosages above 25 mg/kg body weight (bw) in rats and guinea pigs. Groups of male and female rats given daily doses up to 2000 mg/kg bw Ascorbic Acid for 2 years had no macro- or microscopically detectable toxic lesions. Mice given Ascorbic Acid subcutaneous and intravenous daily doses (500 to 1000 mg/kg bw) for 7 days had no changes in appetite, weight gain, and general behavior; a...

show abstract

The effect of antioxidants on bleomycin treatment in in vitro and in vivo genotoxicity assays

Cited by 48 publications

References 41 publications

Protective effects of the amino acid glutamine and of ascorbic acid against chromosomal damage induced by doxorubicin in mammalian cells

Protective effects of the amino acid glutamine and of ascorbic acid against chromosomal damage induced by doxorubicin in mammalian cells

Chemopreventive effect of bilberry (Vaccinium myrtillus) against cisplatin-induced oxidative stress and DNA damage as shown by the comet assay in peripheral blood of rats

Final Report of the Safety Assessment of L-Ascorbic Acid, Calcium Ascorbate, Magnesium Ascorbate, Magnesium Ascorbyl Phosphate, Sodium Ascorbate, and Sodium Ascorbyl Phosphate as Used in Cosmetics1

Contact Info

Product

Resources

About