The effect of asparagus virus infection on asparagus tissue culture

Vries-Paterson, R. M. De; Evans, T. A.; Stephens, C. T.

doi:10.1007/bf00043472

Cited by 16 publications

(8 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…(, ) also found GRLaV‐3 reduced root formation in the virus‐infected in vitro shoots. A number of examples of reduced vegetative growth and rooting can be found in other virus–host combinations such as Asparagus officinalis infected by Asparagus virus I (AV‐I, De Vries‐Paterson et al ., ), Rubus idaeus infected by tobacco streak virus (TSV) or raspberry bushy dwarf virus (RBDV) or tomato ringspot virus (TomRSV) (Tsao et al ., ), Citrus infected by Citrus tristeza virus (CTV) (Wang et al ., ), Musa infected by banana bunchy top virus (BBTV, Haq et al ., ) and potato leafroll virus or potato virus Y (Li et al ., ). All of these studies support ours.…”

Section: Discussionmentioning

confidence: 99%

Development, histological observations and Grapevine leafroll‐associated virus‐3 localisation in in vitro grapevine micrografts

Hao

Cui

et al. 2017

Annals of Applied Biology

View full text Add to dashboard Cite

Development, histological process and Grapevine leafroll-associated virus-3 localisation were studied in micrografts of three scion/rootstock combinations: healthy/healthy, healthy/infected and infected/healthy. Earlier bud break and faster growth in scions of micrografts were obtained when the healthy shoot segments were used as scions, while earlier bud break in rootstocks and greater fresh weight of roots in micrografts were produced when the healthy shoot segments were used as rootstocks. All histological processes including callus initiation and formation in micrografting conjunctions, and initiation of new cambial cells followed by vascular bundle development connecting scions and rootstocks were similar in micrografts, regardless of the sanitary status of the scions and rootstocks used for micrografting. Virus infection in micrografting conjunctions and systematic infection in micrografts were much more efficient and faster in micrografting combination of the infected scions/healthy rootstocks than in the healthy scions/infected rootstocks. To the best of our knowledge, this is the first report addressing histological process of micrograft development and virus localisation in micrografts. In vitro culture system established in this study facilitates studies on the 'pure' impact of the viral infection on micrografting.

show abstract

Section: Discussionmentioning

confidence: 99%

Development, histological observations and Grapevine leafroll‐associated virus‐3 localisation in in vitro grapevine micrografts

Hao

Cui

et al. 2017

Annals of Applied Biology

View full text Add to dashboard Cite

show abstract

“…Igor infected with potato virus Y NTN accumulates jasmonic acid in the roots, and roots are significantly shorter, but there are no significant differences in the average length of infected and healthy potato plantlets (Petrovic et al, 1997). Fresh and dry weights of infected potato shoots and roots are reduced but no in vitro shoot symptoms appear, such as the stunting, necrosis, and mosaic patterns normally observed on field plants (De Vries-Paterson et al, 1992;Petrovic et al, 1997). The only case of virus and virus-like agents affecting both the number and size of shoots produced is in nodal citrus-stem segments (Greno et al, 1988).…”

Section: Resultsmentioning

confidence: 95%

“…It is known that virus-infected tissue can have reduced in vitro growth compared to non-infected tissue. De Vries-Paterson et al (1992) first reported the effects of single and two-virus infections in reducing asparagus in vitro root development, survival in culture, and the fresh and dry weights of micropropagated plants. In vitro shoot-tip culture alone, without other virus-elimination treatments, resulted in only 8.6% virus-free asparagus clones.…”

Section: Introductionmentioning

confidence: 98%

Virus infections reduce in vitro multiplication of ‘Malling Landmark’ raspberry

Tsao

Postman

Reed

2000

In Vitro Cell.Dev.Biol.-Plant

View full text Add to dashboard Cite

Virus-infected plants are often symptomless and may be inadvertently used as explant sources in tissue culture research. Our objective was to determine the effect of virus infection on micropropagation. We studied the effects of single and multiple infections of three common raspberry viruses on the in vitro culture of 'Malling Landmark' red raspberry (Rubus idaeus L.). Virus-infected raspberry plants were produced by leaf-graft inoculation from known-infected plants onto virus-free 'Malling Landmark'. Single-virus source plants were infected with either tobacco streak ilarvirus (TSV), tomato ringspot nepovirus (TomRSV), or raspberry bushy dwarf idaeovirus (RBDV) and were free of other viruses as determined by enzyme-linked immunosorbent assay (ELISA) and bioassay. Virus-free, single, and multiple virus-infected 'Malling Landmark' explants were initiated into culture and multiplied on Anderson's medium with 8.9 mM N 6 -benzyladenine (BA). At the end of the multiplication tests, ELISA reconfirmed virus infections. In vitro multiplication of 'Malling Landmark' was significantly reduced by multiple infections, and multiplication of plants infected with all three viruses (RBDV + TomRSV + TSV) was less than half that of virus-free cultures. Shoot height and morphology of in vitro cultures were not influenced by virus infection. The greenhouse stock plant with the three-virus infection was stunted and yellow compared to the control and the other infected plants.

show abstract

“…Apparently TropMV is an easy virus to eliminate from mashua compared to other plant-virus combinations (De Vries-Paterson et al ., 1992; Zapata et al ., 1995) as 83% of the apical dome-derived plants tested free of TropMV. This may have been due to characteristics of TropMV and/or size of apical dome.…”

Section: Discussionmentioning

confidence: 99%

“…Apical dome culture is one technique that has been used to produce virus-tested germplasm in a number of crops (De Vries-Paterson et al ., 1992; Zapata et al ., 1995). Protocols for initiating and maintaining mashua in vitro have been developed (Estrada et al ., 1986; Perea-Dallos et al ., 1986).…”

Section: Introductionmentioning

confidence: 99%

IN VITRO AND ECUADOR-FIELD PERFORMANCE OF VIRUS-TESTED AND VIRUS-INFECTED PLANTS OF TROPAEOLUM TUBEROSUM

Taboada²,

Gonzalez³

et al. 2009

Ex. Agric.

View full text Add to dashboard Cite

Ecuadorean mashua (Tropaeolum tuberosum) germplasm has been found to be widely infected with the potyvirus Tropaeolum mosaic virus (TropMV). The objective of this research was to produce virus-tested (VT) germplasm to compare growth in vitro and in the field with virus-infected (V) germplasm. Twentythree of 25 apical dome-derived clones tested free of virus based on bioassays using Nicotiana benthamiana and Chenopodium quinoa. In vitro-generated plant tissue was just as effective for determining VT status as greenhouse-generated plant tissue. Genotype rather than virus-infection status appeared to have a greater effect on in vitro proliferation. There were no differences in in vitro rooting among the genotypes or between the VT clones compared to the V clones, with at least 90% of the microcuttings rooting. However, rooted microcuttings of V clones were taller than rooted microcuttings of VT clones. Plants were readily reestablished in a greenhouse at the USDA, Foreign Disease-Weed Science Research Unit at Fort Detrick, USA. In field experiment 1, ca. 75% of the plants survived field transplanting and VT plants of genotype 1147 had greater tuber weight (928 g) than V plants (235 g). In field experiment 2, plant mortality was high one month after field transplanting. Genotypes 1093 (59%) and 1141 (54%) had higher survival than genotype 1147 (44%); however, survival did not differ between the VT (46%) and V (59%) plants of all genotypes. No differences were noted in field performance for the three genotypes after 10 months of growth. Although overall tuber yield among the V, VT and VTR (reinfected-VT plants) did not differ, V plants produced big tubers that weighed more than those from VT plants. Thirty-three percent of the VT plants became reinfected and 42% of the V plants tested negative after 10 months in the field based on double-antibody-sandwich ELISA.

show abstract

The effect of asparagus virus infection on asparagus tissue culture

Cited by 16 publications

References 17 publications

Development, histological observations and Grapevine leafroll‐associated virus‐3 localisation in in vitro grapevine micrografts

Development, histological observations and Grapevine leafroll‐associated virus‐3 localisation in in vitro grapevine micrografts

Virus infections reduce in vitro multiplication of ‘Malling Landmark’ raspberry

IN VITRO AND ECUADOR-FIELD PERFORMANCE OF VIRUS-TESTED AND VIRUS-INFECTED PLANTS OF TROPAEOLUM TUBEROSUM

Contact Info

Product

Resources

About