The effect of Astragalus polysaccharides on attenuation of diabetic cardiomyopathy through inhibiting the extrinsic and intrinsic apoptotic pathways in high glucose -stimulated H9C2 cells

Sun, Shuqin; Yang, Shuo; Dai, Min; Jia, Xiujuan; Wang, Qiyan; Zhang, Zheng; Mao, Yongjun

doi:10.1186/s12906-017-1828-7

Cited by 45 publications

(31 citation statements)

References 45 publications

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“…For instance, the specific polysaccharide receptor on the surface of muscle cells has yet to be identified. Because both β‐glucan and APS, which consists mainly of α‐(1→4), (1→6) dextran, arabinogalactan, rhanmnogalacturonan I, and arabinogalactan proteins (Sun et al, ), activated glucose uptake in muscle cells, this receptor probably recognizes a broad range of polysaccharides. Furthermore, it remains unclear for which enzyme phosphorylates AMPK in the L6 muscle cells.…”

Section: Resultsmentioning

confidence: 99%

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Purification, characterization, and biological activities of purified polysaccharides extracted from the gray oyster mushroom [Pleurotus sajor-caju (Fr.) Sing.]

et al. 2018

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Gray oyster mushroom, a common edible mushroom in Thailand, has been used as a source for polysaccharide extraction and purification. In this study, we extracted polysaccharides from the gray oyster mushroom through hot water, ammonium oxalate, and alkaline extraction methods. The extracts were further purified using enzymatic digestion, solvent extraction, and column chromatography. Fourier‐transform infrared spectroscopy reveals that the selected fractions contain β‐(1→3) glucan and mannan. Monosaccharide composition analysis suggests that glucose and mannose are the major constituents of all analyzed fractions. Interestingly, only the gel‐like fraction, namely SG1‐1 could enhance neutrophils’ killing activity on Candida albicans. In addition, the purified fractions stimulated glucose uptake activity in the L6 myotubes in a dose‐dependent manner. Practical applications The ability of those polysaccharides to stimulate glucose uptake in the muscle cells and enhance a neutrophil’s killing activity implicates the antidiabetic and immunostimulatory activities, respectively. Thus, these polysaccharides could be potentially developed into an immunostimulating agent and an alternative medicine for treating diabetes mellitus.

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Section: Resultsmentioning

confidence: 99%

“…Laminarin S1-1 S1-2 S1-3 SG1-1 SG1-2 AS2-1-1 AS2-1-2 AS2-2 AS4-1 S1-1 S1-2 S1-3 SG1-1 AS2-1 AS2-1-2 AS2-2 AS3-1 AS4- (Sun et al, 2017), activated glucose uptake in muscle cells, this receptor probably recognizes a broad range of polysaccharides.…”

Section: Untreatedmentioning

confidence: 99%

Purification, characterization, and biological activities of purified polysaccharides extracted from the gray oyster mushroom [Pleurotus sajor-caju (Fr.) Sing.]

et al. 2018

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“…In addition, our previous studies revealed that polysaccharides were the main active compounds of CV aqueous extract. Numerous studies have reported that polysaccharides protect against DCM by inhibiting oxidative stress or apoptosis or by activating heart chymase or the NRG1/ErbB pathway (Chang, Lu, Wang, Lv, & Fu, ; Chen et al, ; Sun et al, ). These findings suggest that CV extract has a functional role in protecting heart function in DCM.…”

Section: Discussionmentioning

confidence: 99%

Coriolus versicolor alleviates diabetic cardiomyopathy by inhibiting cardiac fibrosis and NLRP3 inflammasome activation

Wang

Yang

et al. 2019

Phytotherapy Research

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Coriolus versicolor (CV) is a traditional medicine and food mushroom. Our previous study demonstrated that CV extract exhibited anti-hyperglycemia and anti-insulin resistance effects. However, the effect of CV on cardiac function in diabetic cardiomyopathy (DCM) remains unclear. Therefore, we aimed to investigate the effect of CV on cardiac function in diabetes mellitus (DM) rats. We found that the cardiac dysfunction of DM rats was markedly improved by CV extract treatment. CV extract administration significantly attenuated cardiac fibrosis in DM rats, which was accompanied by suppressed transforming growth factor beta 1 (TGF-β1)/Smad signaling as indicated by decreased levels of TGF-β1, p-Smad2, and p-Smad3 and increased Smad7 expression. Moreover, CV extract treatment significantly alleviated cardiac inflammation as shown by decreased levels of NLRP3 receptor, cleaved caspase-1, IL-1β, and IL-18 in DM rats at least partly due to the inhibition of the NF-κB. In addition, high-glucose treatment induced cardiac fibrosis and increased cardiac inflammation in cardiac fibroblast cells, but these effects were ameliorated by CV extract treatment. Therefore, we conclude that the protective effect of CV on DCM is associated with the suppression of TGF-β1/Smad signaling and attenuation of NLRP3 inflammasome activation, suggesting that CV extract may be a potential therapeutic agent for DCM.

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“…Apoptosis is one of the major determining factors for the pathological progression of DM associated organ damages. Apoptosis causes cardiomyocyte loss thereby facilitating cardiac remodeling (Sun et al, ). The severity of diabetes is normally proportional to the levels of cardiac cell death (Ghosh et al, ) and attenuation of apoptosis has shown amelioration of diabetic cardiomyopathy (Tian et al, ; Zhang et al, ).…”

Section: Discussionmentioning

confidence: 99%

“…The reduction in apoptosis event and associated downregulation of the protein mediators of apoptosis was correlated with suppression of TNF‐α. Accumulating evidences suggest that increase in the levels of pro‐inflammatory cytokine TNF‐α is one of the crucial events in DM associated cardiac apoptosis (Sun, Yang, Dai, Jia, Wang, Zhang & Mao, ; Westermann et al, ). VHVV considerably suppressed the markers of apoptosis such as cleaved caspase 3 and cytochrome c in H9c2 challenged with high glucose and elevated the expression of Bcl‐2.…”

Section: Discussionmentioning

confidence: 99%

Short Tetra‐peptide from soy‐protein hydrolysate attenuates hyperglycemia associated damages in H9c2 cells and ICR mice

Shibu

Wang

et al. 2018

J Food Biochem

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Various novel therapeutic interventions are evaluated to overcome hypertrophy and apoptosis associated with diabetic cardiomyopathy. In this study, the anti‐apoptotic and anti‐hypertrophic efficiency of a short peptide composed of Val‐His‐Val‐Val (VHVV) from a soy‐protein hydrolysate was investigated against high glucose (HG) challenge. H9c2 cells were challenges with 33 mM of glucose and showed elevated levels of apoptotic events, pro‐apoptotic proteins, and hypertrophic markers in HG administered H9c2 cells however the abnormalities were efficiently attenuated by VHVV. Further the protective effects of VHVV were further confirmed by administering VHVV on STZ‐induced DM ICR mice. DM ICR mice exhibited notable difference in tissue architecture in liver, heart, and pancreas as determined by Hematoxylin–Eosin staining. Our results conclude that low and high doses of VHVV treatment effectively suppressed the effects of DM in ICR mice by inhibiting the deterioration in gluocgan and insulin secreation in the pancreas indicating enhanced the β‐cell function. Practical applications Practical applications Soy‐protein, isolated from soybean meal, has been consumed for several decades for its functional properties and is also a regular constituent of various food supplements. The functional properties of soy‐protein include its positive effects on serum cholesterol, low‐density lipoprotein cholesterol, and triglyceride levels. Soy‐protein has been demonstrated to possess protection against diabetes associated damages in human. The results of the present study indicate that VHVV, a short peptide derived from soy‐protein hydrolysate is an active anti‐diabetic principle in the soy‐protein. The finding identifies a novel form of antidiabetic peptide that can be used as functional ingredients in food supplements for debates management and health care.

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The effect of Astragalus polysaccharides on attenuation of diabetic cardiomyopathy through inhibiting the extrinsic and intrinsic apoptotic pathways in high glucose -stimulated H9C2 cells

Cited by 45 publications

References 45 publications

Purification, characterization, and biological activities of purified polysaccharides extracted from the gray oyster mushroom [Pleurotus sajor-caju (Fr.) Sing.]

Purification, characterization, and biological activities of purified polysaccharides extracted from the gray oyster mushroom [Pleurotus sajor-caju (Fr.) Sing.]

Coriolus versicolor alleviates diabetic cardiomyopathy by inhibiting cardiac fibrosis and NLRP3 inflammasome activation

Short Tetra‐peptide from soy‐protein hydrolysate attenuates hyperglycemia associated damages in H9c2 cells and ICR mice

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