The effect of autogenous tooth bone graft material without organic matter and type I collagen treatment on bone regeneration

Abstract

Objectives The aim of this study is to examine the effect of particulate autogenous tooth graft removed with organic matter and type I collagen addition on bone regeneration and to validate the possibility of useful allograft material for jaw defects. Material and methods Autogenous tooth bone maker (Korean Dental Solution® KOREA) made particulate autogenous tooth not including organic matter. We used to the developed tooth grafts for experiment. Cell adhesion test with hemacytometer and energy dispersive X-ray spectroscopy (Supra40 VP®, Carl Zeiss, Germany) analysis about the particulate autogenous tooth and type I collagen were performed. Rabbits were divided into three groups: bone graft with organic matter (OM) removing particulate autogenous tooth group, bone graft with OM removing particulate autogenous tooth and type I collagen group, and a control group. Bone grafting was performed in rabbit’s calvaria. The rabbits were sacrificed at different interval at 1, 2, 4, and 6 weeks after bone grafting for the histopathologic observation and observed the effect of bone regeneration by SEM, H-E & Masson stains, osteocalcin IHC staining. Result In vitro cytopathological study showed affinity for cells, cell attachment pattern, and cell proliferation in the order of control group, OM-removed and collagen-treated group, OM-removed particulate autogenous tooth group. The results of the degree of mineralization were opposite to those of the previous cell experimental results, and the OM-removed group, OM-removed group and collagen-treated group were relatively higher than the control group. Histopathologic analysis showed that vascularization and neonatal bone formation were higher in particulate autogenous tooth group with removing OM and with addition of collagen than control group and group of OM removed only. Immunohistochemical analysis showed that osteocalcin (OSC) expression was not observed in the control group, but at 4 weeks groups, OSC expression was observed the OM removed and OM-removed-collagen-treated particulate autogenous tooth, and the degree of expression was somewhat stronger in group of the OM removed and collagen additionally treated particulate autogenous tooth. Conclusion Particles that do not contain organic matter, the saint tooth, was responsible for sufficient bone graft material through the role of space maintenance and bone conduction, and further improved bone formation ability through additional collagen treatment. Therefore, research on various extracellular substrates and autologous bone grafting materials is necessary, and through this, it is possible to lay the foundation for a new type of autologous bone grafting material with excellent academic and technical utility.

Alveolar ridge preservation in sockets with severe periodontal destruction using autogenous partially demineralized dentin matrix: A randomized controlled clinical trial

“…Besides, APDDM exhibits good biocompatibility, owing to its structural components, which are composed of low‐crystalline hydroxyapatite (HA) and other calcium phosphate minerals, similar to human bone tissue 51 . The inorganic component of APDDM plays a critical role in space maintenance and osteoconduction, while the organic component supports cells and proteins for bone healing and promotes vascularization 52 . In addition, APDDM contains various growth factors, such as transforming growth factor β, bone morphogenetic protein (BMP), and vascular endothelial growth factor, 53 which induce osteoblastic differentiation of stem cells, promote the proliferation and migration of osteoblasts and osteocytes, and accelerate vessels formation, stimulating bone deposition by regulating gene expression 54 .…”

“…51 The inorganic component of APDDM plays a critical role in space maintenance and osteoconduction, while the organic component supports cells and proteins for bone healing and promotes vascularization. 52 In addition, APDDM contains various growth factors, such as transforming growth factor β, bone morphogenetic protein (BMP), and vascular endothelial growth factor, 53 which induce osteoblastic differentiation of stem cells, promote the proliferation and migration of osteoblasts and osteocytes, and accelerate vessels formation, stimulating bone deposition by regulating gene expression. 54 The noncollagenous proteins present in DDM, such as dentin sialoproteins 51 and dentin phosphoproteins, are also involved in bone formation.…”

Long-Term Changes in Adipose Tissue in the Newly Formed Bone Induced by Recombinant Human BMP-2 In Vivo

“…This led to a time-dependent increase in BMD and TbTh in the particle groups. Bone grafting surgery for implant installation has been widely performed in patients with alveolar BV deficiency [ 37 ]. Popularly used with bone grafting material, rhBMP-2 successfully induces new bone regeneration in guided bone regeneration (GBR), maxillary sinus augmentation, and bone defects after cyst enucleation [ 23 , 38 ].…”

CGF-HLC-I repaired the bone defect repair of the rabbits mandible through tight junction pathway

“…However, due to its high extraction cost, the research on it has only achieved a simplified bionic structure ( Liao et al, 2021 ). Mingu K et al found that collagen played a role in bone formation in the repair of calvarial defects in rabbits ( Kim et al, 2021 ). Bio-Gide combined with Bio-Oss, a resorbable bone filler, can be used for bone regeneration and repair ( Zhao et al, 2019 ).…”