The Effect of Avidin on Viability and Proliferation of Colorectal Cancer Cells HT-29

Abstract

Objective:The aim of this study was to analyze the effect of avidin treatment on cell viability, proliferation and cyclin D1 expression in colorectal cancer cells HT-29. Methods: Colorectal cancer cell line HT-29 incubated with 50, 100, 150, and 200 μg/mL of avidin concentration during 24, 48, and 72 hours, then the cell viability and proliferation were analyzed. Each avidin concentration was conducted together with HT-29 cell line without avidin treatment as a control group. The cell viability was measured by MTS assay and the proliferation was measured by BrdU (5-bromo-2′-deoxyuridine) cell proliferation assay. According to cell viability and proliferation result, we determined the 100 μg/ mL avidin concentration for analyzing mRNA and protein of cyclin D1. Results: We demonstrated that the viability and proliferation of HT-29 cells were significantly decreased in all concentration of avidin treatment compared to control. The cell proliferation showed larger reduction in avidin treatment rather than cell viability. This proves avidin could inhibit proliferation of colorectal cancer cell HT-29 quite well. The expression of cyclin D1, both mRNA and protein, was also significantly decreased after the avidin treatment group compared to control group, it supports the suppression of proliferation result. Conclusion: We concluded that avidin treatment could decrease cell viability and proliferation, accompanied by suppression of cyclin D1 expression in colorectal cells HT-29.

Cytotoxic and apoptotic effects of Prunus spinosa fruit extract on HT-29 colon cancer line

“…The new medium contained herb extracts at different concentrations. No extract was added in the control group (Dewi et al, 2022). To determine IC20, IC50, and IC80 values, cells were exposed to different concentrations of the water extract of P. spinosa for 24 and 48 hours (43.95, 87.89, 175, 351.56, 703.13, 1406.2, 2812.5, 5625, 11250, 22500 and 45000 µg/ml).…”

In vitro-simulated intestinal flora fermentation of Porphyra haitanensis polysaccharides obtained by different assisted extractions and their fermented products against HT-29 human colon cancer cells

“…HT-29 cells possess the characteristics of mature intestinal cells, and thus can be used to study the anti-colon-cancer activities of substances. 22,23 Dewi et al investigated the anticolon cancer effect of avidin using colorectal cancer HT-29 cells and found that avidin can reduce the viability of the HT-29 colon cancer cell line and inhibit cell proliferation. 23 Therefore, in this study, PHPs were prepared via water extraction, ultra-high-pressure-, ultrasonic-and microwave-assisted water extraction.…”

“…22,23 Dewi et al investigated the anticolon cancer effect of avidin using colorectal cancer HT-29 cells and found that avidin can reduce the viability of the HT-29 colon cancer cell line and inhibit cell proliferation. 23 Therefore, in this study, PHPs were prepared via water extraction, ultra-high-pressure-, ultrasonic-and microwave-assisted water extraction. The effects of PHPs on the intestinal flora and SCFAs were determined during in vitro-simulated human intestinal flora fermentation.…”