The Effect of Bathophenanthroline and Hydroxylamine

Abstract

Bathophenanthroline has no effect on the activity of latent Mg^2+-dependent ATPase in human erythrocytes, but inhibits that of saponin-stimulated enzyme (K(i) = 1.5 x 10^-2 mol/l). Hydroxylamine takes a different action. Enzyme activity increases with increasing concentration of hydroxylamine, reaching its peak at approximately 10 mmol/l; a further increase in concentration results in gradual decrease of activity. Saponin-stimulated activity of Mg^2+-dependent ATPase is inhibited; a half maximum activity is reached at approx. 60 mmol/l hydroxylamine.