The effect of biological sealants and adhesive treatments on matrix metalloproteinase expression during renal injury healing

Abstract

BackgroundRenal injuries are relatively common in cases of abdominal trauma. Adhesives and sealants can be used to repair and preserve damaged organs. Using a rat model, this study explores the activity of different matrix metalloproteinases (MMP) during the healing of renal injuries treated by two biological adhesives (TachoSil and GelitaSpon) and a new synthetic elastic cyanoacrylate (Adhflex).MethodsRenal traumatic injuries were experimentally induced in 90 male Wistar rats by a Stiefel Biopsy Punch in the anterior aspect of the left kidney. Animals were divided into five groups: 1, sham non-injured (n = 3); 2, non-treated standard punch injury (n = 6); 3, punch injury treated with TachoSil (n = 27); 4, punch injury treated with GelitaSpon (n = 27); and, 5, punch injury treated with Adhflex (n = 27). Wound healing was evaluated 2, 6, and 18 days after injury by determining the expression of MMPs, and the histopathological evolution of lesions.FindingsHistologically, the wound size at 6 days post-injury was larger in Adhflex-treated samples than in the other treatments, but the scarring tissue was similar at 18 days post-injury. Only the MMPs subtypes 1, 2, 8, 9, and 13 were sufficiently expressed to be quantifiable. Both time since injury and treatment type had a significant influence on MMPs expression. Two days after injury, the expression of MMP8 and MMP9 was predominant. MMP2 expression was greater 6 days after injury. The Adhflex-treated group had a significantly higher MMPs expression than the other treatment groups at all healing stages.ConclusionsAll three sealant treatments induced almost similar expression of MMPs than untreated animals indicating a physiological healing process. Given that all renal trauma injuries must be considered emergencies, both biological and synthetic adhesives, such as Adhflex, should be considered as a treatment options.

Magnetic responsive materials modulate the inflammatory profile of IL-1β conditioned tendon cells

“…increased in IL-1 β treated hTDCs but, when cells were exposed to PEMF the MMPs levels decreased. The expression of MMPs, enzymes implicated in cell responses to the environmental cues, including cell proliferation, migration, differentiation and in the dynamic process of ECM turnover, with relevant implications in the inflammatory and remodeling phases of healing [33] , suggest that PEMF actuation can modify hTDCs behavior, decreasing the expression of MMPs associated to collagen degradation, which may contribute to inflammatory pain and tendon lesions. As cytoskeleton changes often precede MMP modulation during tissue remodeling, actin dynamics might be linked to the expression of MMP genes [34] .…”

Contribution of Autologous Omentum Transposition to the Regeneration of Renal Injuries in the Rat Model

“…In groups 3 and 4 (kidney omentum repair groups), transpositioned autologous omentum was used without primary sutures on the injured renal tissue for repair. We preferred the time of sacri ce as the 18 th postoperative day, according to the study of Miguel et al (14) which reported that posttraumatic necrosis in the tissue disappeared on the 18 th day. In that study, after this period, collagen maturation took place in the renal capsule and connective tissue at the edges of the wound were contracted (14).…”

“…We preferred the time of sacri ce as the 18 th postoperative day, according to the study of Miguel et al (14) which reported that posttraumatic necrosis in the tissue disappeared on the 18 th day. In that study, after this period, collagen maturation took place in the renal capsule and connective tissue at the edges of the wound were contracted (14). The summary of the procedures performed in the study groups are outlined in Table 1.…”

Matrix Metalloproteinases in Renal Diseases: A Critical Appraisal

“…Experimental studies provide evidence that glucocorticoids mediated the transcriptional suppression of cytokine-induced MMP-9 expression in rat mesangial cells [41, 42]. A recent article exploring the effect of biological sealants and adhesive treatments on MMP expression during kidney damage healing found that the expression of collagenases (MMP-1, MMP-8, and MMP-13) and subsequently the expression of gelatinases (MMP-2 and MMP-9) occur at different tested time periods [43]. This overexpression of metalloproteinases at the early healing period obviously does not influence the final kidney repair [43].…”

“…Different time manner was specifically prominent with maximal MMP-8 and MMP-9 expression after 2 days of injury in healing of the wound. In contrast, the MMP-2 expression peaked after 6 days of kidney traumatic injury [43]. …”