The Effect of Busulfan on Body Weight, Testis Weight and MDA Enzymes in Male Rats

Nasibeh, Hosseini Ahar; Khaki, Arash; Akbari, Ghasem; Marefat, Ghaffari Novin

doi:10.15296/ijwhr.2014.52

Cited by 31 publications

(20 citation statements)

References 9 publications

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“…Moreover, the study by Hosseini Ahar et al showed that the use of busulfan can reduce the body weight and testicular weight in male rats [29], which is consistent with our results. Zheng Wei et al also showed a direct relationship between the testis weight and germinal cells number [30].…”

Section: Discussionsupporting

confidence: 93%

The effects of ellagic acid on testicular tissue changes, sexual hormones, antioxidant system and Gene Expression of Caspase-9 and Bcl-2 in the relative sterility rat model following administration of busulfan: A stereological study

Vakili¹,

Koohpeyma²,

Saki³

et al. 2020

Preprint

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Background Busulfan is an antineoplastic medication that is broadly utilized for cancer treatment. On the other hand, prescription of busulfan may cause sterility in male patients. Therefore, the decrease of this side effect is important. The aim of the present study was to evaluate the effects of ellagic acid on testicular tissue changes, sexual hormones, antioxidant defense system, and caspase-9 and Bcl2 gene expression in the relative sterility rat model following administration of busulfan.Methods Rats were randomly assigned to five groups of 13 animals per group. Sterility was induced by a single injection of busulfan (10 mg/kg) in groups 3, 4 and 5. The control group was not treated. The healthy group received 50 mg/kg ellagic acid. Groups 4 and 5 (treatment group) received 10 mg/kg and 50 mg/kg ellagic acid, respectively for 48 days. Then, the serum levels of antioxidant enzymes, Malondialdehyde, sexual hormones and the testicular damage were evaluated.Results The significant increment of total antioxidant capacity and catalase was seen in both treatment groups (p < 0.001). Also, both treatment groups significantly increased spermatogonia, round spermatids and long spermatids. Treatment with 50 mg/kg ellagic acid significantly increased the testis weight, testis volume, seminiferous tubule volume, germinal epithelium volume, interstitial tissue volume, spermatocyte, Sertoli cells, and Leydig cells in the busulfan group(P < 0.05). Additionally, 50 mg/kg ellagic acid significantly increased the gene expression of Bcl2 and decreased caspase 9 in the busulfan group (P < 0.05).Conclusions The consumption of ellagic acid may have beneficial effects on antioxidant defense system, sexual hormones abnormality and testicular tissue damage.

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Section: Discussionsupporting

confidence: 93%

The effects of ellagic acid on testicular tissue changes, sexual hormones, antioxidant system and Gene Expression of Caspase-9 and Bcl-2 in the relative sterility rat model following administration of busulfan: A stereological study

Vakili¹,

Koohpeyma²,

Saki³

et al. 2020

Preprint

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“…Most synthetic anticancer agents have been associated with nephrotoxic [136,137], neurotoxic, cardiotoxic [138] and gonadotoxic [139,140] side effects. For this reason, the search for anti-cancer bioactive peptides from food sources has increased.…”

Section: Food-derived Bioactive Peptides and Human Healthmentioning

confidence: 99%

Bioactive Peptides

Daliri

Lee

2017

Foods

423

254

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The increased consumer awareness of the health promoting effects of functional foods and nutraceuticals is the driving force of the functional food and nutraceutical market. Bioactive peptides are known for their high tissue affinity, specificity and efficiency in promoting health. For this reason, the search for food-derived bioactive peptides has increased exponentially. Over the years, many potential bioactive peptides from food have been documented; yet, obstacles such as the need to establish optimal conditions for industrial scale production and the absence of well-designed clinical trials to provide robust evidence for proving health claims continue to exist. Other important factors such as the possibility of allergenicity, cytotoxicity and the stability of the peptides during gastrointestinal digestion would need to be addressed. This review discusses our current knowledge on the health effects of food-derived bioactive peptides, their processing methods and challenges in their development.

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“…Our data revealed a signi cant decrease in the rats' body weights with a marked increase in the lung index in the BUS group. The increase in the weight of the lung indicates the occurrence of pulmonary brosis which may be explained by transferring the alkyl group(s) which present in BUS to different body cells to induce lung injury in the form of brosis (Ahar et al 2014). On the other hand, the administration of ALA to the brotic rats exhibited a signi cant elevation in the bodyweight with a marked decline in the lung index.…”

Section: Discussionmentioning

confidence: 99%

Modulation of COX-2 and NADPH oxidase-4 by Alpha-lipoic acid ameliorates busulfan-induced pulmonary injury in rats.

Elhadidy

Elmasry

Elsayed

et al. 2021

Preprint

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purpose: Busulfan is an antineoplastic drug that produces pulmonary fibrosis. This study aimed to explore the potential protective effect of α-lipoic acid on busulfan-induced pulmonary fibrosis in rats.Methods: Twenty-four adult male rats were divided into four groups: control, α-lipoic acid (ALA), busulfan, and busulfan plus α-lipoic acid. Lung index ratio, serum level of proinflammatory cytokine were assessed. The activities of antioxidant enzymes and lipid peroxidation products were estimated in the lung tissues in addition to histopathological analyses. The deposition of the collagen in the lung tissues was evaluated by Sirius red staining. The expressions of α-smooth muscle actin (α-SMA), TNF-α, and Caspase 3 were determined immunohistochemically. The pulmonary expression of COX-2 and NOX-4 mRNA were assessed using qRT-PCR.Results: administration of ALA significantly ameliorated BUS-induced pulmonary fibrosis, besides the upregulation of antioxidants, and downregulation of pro-inflammatory cytokines. Also, it reduced collagen deposition associated with a decreased expression of α-SMA, TNF-α, and Caspase 3 in the lung tissues. Moreover, ALA significantly upregulated the expression of COX-2 concomitant with the downregulation of elevated NOX-4.Conclusion: ALA attenuates the lung cytotoxicity of busulfan through its anti-inflammatory, anti-apoptotic, and antifibrotic effects that may be mediated by upregulation of COX-2 and downregulation of NOX-4.

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The Effect of Busulfan on Body Weight, Testis Weight and MDA Enzymes in Male Rats

Cited by 31 publications

References 9 publications

The effects of ellagic acid on testicular tissue changes, sexual hormones, antioxidant system and Gene Expression of Caspase-9 and Bcl-2 in the relative sterility rat model following administration of busulfan: A stereological study

The effects of ellagic acid on testicular tissue changes, sexual hormones, antioxidant system and Gene Expression of Caspase-9 and Bcl-2 in the relative sterility rat model following administration of busulfan: A stereological study

Bioactive Peptides

Modulation of COX-2 and NADPH oxidase-4 by Alpha-lipoic acid ameliorates busulfan-induced pulmonary injury in rats.

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