The Effect of Caffeine on the Survival of U.V.-irradiated Diploid and Tetraploid Chinese-hamster Cells

Rommelaere, Jean; Errera, Maurice Leo

doi:10.1080/09553007214551061

Cited by 19 publications

(3 citation statements)

References 25 publications

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“…A number of clinical observations (De, 1961;Atkin et al, 1959) as well as some in vivo and in vitro radiobiological studies (Revesz & Norman, 1960;Puck, 1960;Berry, 1963) number and ploidy of cells having diploid, tetraploid or octoploid values. Our findings are in agreement with other in vitro studies (Till, 1961;Lockart et al, 1961;Rommelaire & Errera, 1972;Limbosh et al, 1974;Millar & Millar, 1977) which indicate that chromosome number per se does not correlate with radiation response.…”

Section: Discussionsupporting

confidence: 93%

Response to X-radiation and cytotoxic drugs of clonal subpopulations of different ploidy and metastatic potential isolated from RIF-1 mouse sarcoma

Reeve¹,

Wright²,

Twentyman³

1983

Br J Cancer

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Summary Clonal subpopulations of different ploidy values and metastatic capacities, isolated from the RIF-1 mouse sarcoma, have been tested for in vitro X-radiation sensitivity, for in vitro sensitivity to adriamycin and for in vitro and in vivo sensitivity to melphalan and CCNU. Following X-radiation, no consistent differences in the survival curve characteristics (Do and n) of diploid, tetraploid and octoploid cells were observed. In addition no relationship between radiation response and metastatic capacity was observed. For drug response, no marked differences were found in the dose response curves of RIF-1 clones treated in vitro with adriamycin. However, a wide variation in the responses of RIF-I clones to in vitro melphalan treatment was observed which was independent of both ploidy and metastatic capacity. Although the responses of RIF-1 clones to in vitro CCNU treatment were similarly independent of metastatic capacity, a clear relationship between CCNU sensitivity and ploidy was observed. Thus, all diploid RIF-l clones were markedly more sensitive to CCNU treatment than either tetraploid or octoploid RIF-l clones. For both melphalan and CCNU treatment the relative sensitivities in vitro correlated with in vivo sensitivities as assayed by clonogenic cell survival.

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Section: Discussionsupporting

confidence: 93%

Response to X-radiation and cytotoxic drugs of clonal subpopulations of different ploidy and metastatic potential isolated from RIF-1 mouse sarcoma

Reeve¹,

Wright²,

Twentyman³

1983

Br J Cancer

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“…As discussed in the introduction, Rommelaere and Errera (1972) found that tetraploid cells had a larger decrease in survival when irradiated in the presence of caffeine than the diploid cells. This could be due to the increased relative importance of repair in the tetraploid cells.…”

Section: Freezing Effectsmentioning

confidence: 86%

“…al. , 1958) The results of Rommelaere and Errera (1972) suggested thi1t if repair could be reduced, the differences between the dose-response curves of different ploidy cells could be enhanced. Winans and Dewey (1972) showed that repair of potentially lethal damage was inhibited when cells are in hypertonic media.…”

Section: Curve Fittingmentioning

confidence: 99%

Radiation induced reproductive death as a function of mammalian cell ploidy

Philbrick¹

1976

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Mannnalian cells containing different multiples of the diploid •.chromosome set were created through drug induction and cell fusion. In all cell strains used the chromosome nlli!lber was determined from metaphase spreads, as well• as from DNA content and cell size. The •survival of cells as a function of radiation dose was determine~! for cell lines with differing chromosome complements at 37°C, 4°C, in hypertonic media, while frozen, and with increasing levels of incorporated IUdR. Survival of frozen diploid and hypotetraploiu Chinese hamster cells was determined following varying. mnnhers of. 3 125. decays of :mcorporated HfdR and IUdR. The percent of reproductively viable cells following irradiation is a function of the cell ploidy, i.e. the n~ber of haploid sets of chromosomes contained in the cell genome. At 37°C and in hypertonic. media, the Chinese hamster cells of progressively higher rHoidies are ' .1 ' ,. increasingly sensitive to irradi~t~on. As the number of chromosomes per unit cell volume increases the radiosensitivity increases. Both trends suggest interaction between chromosomes as an importcu}t cause of cell death.

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Investigations on the frequency of chromosome aberrations in bone marrow cells of Chinese hamsters after simultaneous application of caffeine and cyclophosphamide

Röhrborn

Buckel

1976

Hum Genet

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The potentiating effect of caffeine (1,3,7-trimethylxanthine) on chemically induced chromosome aberrations was studied in bone marrow cells of chinese hamsters, exposed to the alkylating agent cyclophosphamide. Four experimental series were performed: In the first two tests caffeine (200 mg/kg) or cyclophosphamide (40 mg/kg), respectively, were administered. A third and fourth test was performed with caffeine plus cyclophosphamide (200 + 40 mg/kg and 35 + 40 mg/kg, respectively) simultaneously. Aberrations induced by cyclophosphamide (40 mg/kg) were strongly potentiated by simultaneous application of caffeine (200 mg/kg) not only additively but even synergistically. This increase of aberrations cannot be found after injection of the lower dose of caffeine (35 mg/kg).

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The Effect of Caffeine on the Survival of U.V.-irradiated Diploid and Tetraploid Chinese-hamster Cells

Cited by 19 publications

References 25 publications

Response to X-radiation and cytotoxic drugs of clonal subpopulations of different ploidy and metastatic potential isolated from RIF-1 mouse sarcoma

Response to X-radiation and cytotoxic drugs of clonal subpopulations of different ploidy and metastatic potential isolated from RIF-1 mouse sarcoma

Radiation induced reproductive death as a function of mammalian cell ploidy

Investigations on the frequency of chromosome aberrations in bone marrow cells of Chinese hamsters after simultaneous application of caffeine and cyclophosphamide

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