The effect of chemical treatment of the sheep embryo zona pellucida on the ability of blastocysts to hatch after vitrification and warming

Mousavi, Fatemeh-Sadat; Ahmadi, Ebrahim; Shirazi, Abolfazl; Shams‐Esfandabadi, Naser; Nazari, Hassan

doi:10.1002/vms3.632

Cited by 3 publications

(1 citation statement)

References 30 publications

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“…The introduction of chemical methods, especially the use of acidic Tyrode’s solution, has shown good results and affects the implantation rate by promoting embyro hatching [ 2 ]. In addition, recent technological advances, including the use of non-contact diode lasers, indicate the development of techniques that have the potential to further enhance growth by opening and keeping the zona pellucida open [ 48 ]. This comprehensive study highlights the various aspects of AH technology and its impact on different crops, providing insights to practitioners and researchers working to improve treatment outcomes when transitioning from ART.…”

Section: Reviewmentioning

confidence: 99%

Unlocking the Potential of Assisted Hatching in Assisted Reproductive Technology: A Narrative Review

Deotalu,

More,

Karadbhajne

et al. 2024

Cureus

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The present study is set in the broad field of assisted reproductive technologies (ARTs) and examines various procedures under assisted hatching (AH). It also reviews their effects on implantation success rates. The primary emphasis is on explaining who has benefited and how many have benefited from these interventions. The most important factor determining the success rate of ART is implantation. To increase these rates, we use AH in our clinics to enhance each embryo’s chances at life and substantially improve overall results. This comprehensive review includes various approaches, such as chemical-based measures (such as applying Tyrode’s solution) and mechanical techniques (such as zona drilling and partial zona dissection). The individual techniques are carefully scrutinized, considering their mechanical detailing, methods of applying therapeutic effects, and the appropriateness of matching present social circumstances. The review begins by analyzing the basic nature of AH as a medium for embryo implantation and then focuses on how this detailed view reveals the advantages and drawbacks of various methods. Moreover, the articles discuss improvements in AH technology and many of the most modern technological developments that can help fine-tune ART issues. A major problem with these methodologies is that they involve serious risks and legal complications. However, a broad assessment of these topics allows us to understand their impact on fertility treatments. This review is written as a guidebook for physicians and researchers working in reproductive medicine. It compiles all current knowledge, providing literature to build on successes that will make breakthroughs possible in ART. Indeed, this is a valuable reference for guiding us in navigating complex AH procedures. It advances ARTs step by step toward perfection.

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Section: Reviewmentioning

confidence: 99%

Unlocking the Potential of Assisted Hatching in Assisted Reproductive Technology: A Narrative Review

Deotalu,

More,

Karadbhajne

et al. 2024

Cureus

View full text Add to dashboard Cite

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The effect of chemical treatment of the sheep embryo zona pellucida on the ability of blastocysts to hatch after vitrification and warming

Mousavi

Ahmadi

Shirazi

et al. 2021

Veterinary Medicine & Sci

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Background:The embryo release from the zona pellucida is of prerequisites of successful implantation.Objectives: Regarding the negative impact of embryo cryopreservation on the blastocysts hatchability, the aim of the present study was to investigate the effects of treating embryonic zona pellucida with pronase or acidic Tyrode's solution (ATS) before morula formation on the viability, freezability, and hatchability of vitrified-warmed resulted blastocysts. Methods:In the first experiment, the zona pellucida of 3-and 4-day-old embryos were treated with the above compounds for 30 or 45 s. Then, the competency of the treated embryos to reach to blastocyst stage and the hatchability of resulting blastocysts were investigated. In the second experiment, the cryo-survivability and hatching rate of blastocysts resulting from 3-day-old embryos treated with pronase and ATS for 30 s were tested. Results:In the first experiment and in contrast to the 45 s exposure, 30-s exposure of embryos to pronase or ATS did not have negative effect on the viability and development of embryos to blastocyst stage. In the second experiment, the freezability of blastocysts derived from 3-day-old embryos treated with pronase and ATS for 30 s was not different from that of the control group. However, the hatching rate of the pronase group was significantly higher than that of the control group. Conclusion:The results of the present study showed that reducing the thickness of zona pellucida of sheep embryos with pronase had no negative effect on the developmental competency and freezability of the treated embryos and improved the hatchability of vitrified-warmed blastocysts.

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Optimization of handmade cloning technique in sheep and preliminary investigation of umbilical cord mesenchymal stem cells as donor nuclei

Li,

Liu,

Wang

et al. 2024

Reprod Domestic Animals

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Handmade cloning (HMC) has a higher yield and is relatively less difficult to operate compared to traditional micromanipulation cloning. Yet, there are few reports on handmade cloning in sheep. Therefore, this study investigates the key nodes such as AC and DC voltage, denucleation method and fusion method in sheep handmade cloning. In addition, it compares the effects of fibroblasts (FC) and umbilical cord mesenchymal stem cells (UC‐MSCs) of different states as donors on the development of HMC embryos. Furthermore, the effect of different freezing solutions on the survival rate of frozen blastocysts without zona pellucida was also investigated. The results indicate that an AC voltage of 150 V/cm and a DC voltage of 1800 V/cm significantly enhanced the fusion and blastocyst rates (p < .01). The blastocyst rate achieved with umbilical cord MSCs as nucleus donors was significantly higher (40.3%) than that achieved with fibroblasts and differentiated umbilical cord MSCs (21.5%, 22.5%) (p < .01). The highest survival rate was achieved using 20% DMSO + 20% EG for freezing without zona pellucida. In conclusion, the most efficient and pregnant ovine HMC cloning method using 150 V/cm AC, 1800 V/cm DC, knife‐cut denucleation, two‐step fusion and the use of UC‐MSCs as nucleus donors resulted in the highest overall efficiency and pregnancy after transplantation.

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The effect of chemical treatment of the sheep embryo zona pellucida on the ability of blastocysts to hatch after vitrification and warming

Cited by 3 publications

References 30 publications

Unlocking the Potential of Assisted Hatching in Assisted Reproductive Technology: A Narrative Review

Unlocking the Potential of Assisted Hatching in Assisted Reproductive Technology: A Narrative Review

The effect of chemical treatment of the sheep embryo zona pellucida on the ability of blastocysts to hatch after vitrification and warming

Optimization of handmade cloning technique in sheep and preliminary investigation of umbilical cord mesenchymal stem cells as donor nuclei

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