The Effect of Chick Embryo Cerebro-Spinal Fluid in Microwave Irradiated Collagen Guide Channel on Sciatic Nerve Regeneration in Rat

Abstract

The aim of this research was to evaluate the effect of chick embryo cerebrospinal fluid (e-CSF) across the irradiated collagen guide channel on sciatic nerve regeneration in comparison with autograft. Forty adult male Sprague-Dawley rats (250-300g) were randomized into (1) collagen channel + E-CSF, (2) collagen channel + normal saline (NS), (3) autograft (AG), and (4) sham surgery groups. The left sciatic nerve was exposed and a 10mm nerve segment was cut and removed. In the collagen groups, the proximal and distal cuts ends of sciatic nerve were telescoped into the nerve guides and E-CSF or NS injected into collagen conduit. To the autograft group, the 10 mm nerve segment was turned backwards and used an autologous nerve graft. All animals were evaluated by sciatic functional index (SFI) and electrophysiology testing. The improvements in SFI since the beginning of the last evaluation in experimental groups were measured. On days 49 and 60 post-operation, the mean SFI of the collagen + E-CSF group was significantly greater than the autograft group (P< 0.05). On day 90, the mean nerve conduction velocity (NCV) of the collagen + CSF group was greater than autograft group (P< 0.05). These findings showed that RPS in collagen nerve guide channel effectively enhances nerve regeneration and promotes functional recovery in injured sciatic nerve of rats.

Aloe Vera/Collagen Mixture Induces Integrin α1β1 and PECAM-1Genes Expression in Human Adipose-Derived Stem Cells

“…In this study, type I collagen was extracted from the tail tendons of the rat according to the previously published method. 14 For sterilization of collagen, we carefully transferred the solution to a bottle with a screw cap and containing CHCl 3 (Chloroform) - 10% of the volume of extracted collagen - at the bottom and allowed to rest 12 h at 4°C and then aseptically removed the collagen solution. 15…”

“…Collagen gel, Aloe vera gel, and their blend distributed on 6-well culture plates after adjusting pH in 7.4. 14 Four groups of hASCs were cultured on collagen gel, Aloe vera gel, Aloe vera/collagen blended, and hASCs without any gel. The cells of all groups were grown to confluence.…”