The effect of chloramphenicol resistance mutations on the nodulation competitiveness of Rhizobium leguminosarum biovar Tripolii

Spontaneous streptomycin-resistant mutants were isolated from two fast growing gum-producing strains Ca85 and Ca401 and from two moderately growing strains Ca181 and Ca534 of Rhizobium sp. Cicer. The nodulation ability and symbiotic effectiveness of the mutants relative to parent strains were evaluated on chickpea (Cicer arietinum) grown in sterilized chillum jars. Some mutants of strains Ca85 and Ca401 showed Nod phenotype whereas some mutants of strains Ca181 and Ca534 showed Nod(+) fix(-) phenotype. Other mutants also showed decreased nodule number and reduction in nitrogenase activity as well as in shoot dry weight as compared to inoculation with parental strains. The results showed that acquisition of streptomycin resistance in Rhizobium sp. Cicer strains is associated with decreased symbiotic effectiveness in chickpea, suggesting that antibiotic-resistant mutants first should be analyzed for symbiotic effectiveness before using these mutants for ecological studies or nodulation competitiveness.

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The effect of chloramphenicol resistance mutations on the nodulation competitiveness of Rhizobium leguminosarum biovar Tripolii

Cited by 4 publications

References 17 publications

Effect of mutations in aromatic catabolism on the persistence and competitiveness of Rhizobium legvminosarum bv. trifolii

Effect of mutations in aromatic catabolism on the persistence and competitiveness of Rhizobium legvminosarum bv. trifolii

Competitiveness and symbiotic effectiveness of a R. gallicum strain isolated from root nodules of Phaseolus vulgaris

Symbiotic effectiveness of spontaneous antibiotic-resistant mutants of Rhizobium s p. Cicer nodulating chickpea (Cicer arietinum)

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