The effect of cholesterol loaded cyclodextrins on post-thawing quality of buffalo semen in relation to sperm DNA damage and ultrastructure

Ezz, Mohamed Aboul; Montasser, Abd Elmonem; Hussein, Mamdouh; Eldesouky, Ashraf; Badr, M. A.; Hegab, Abd El-Raouf; Balboula, Ahmed Z.; Zaabel, Samy

doi:10.1016/j.repbio.2016.12.001

Cited by 18 publications

(9 citation statements)

References 44 publications

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“…Skim milk contains sulfhydryl, β -lactoglobulin, phosphocaseinate, and casein micelle, which protect the plasma membrane from lipid peroxidation [ 37 ]. Egg yolk is rich in cholesterol [ 38 ], which improves the quality of stored semen by decreasing mitochondrial deterioration and inhibiting ROS production [ 39 ].…”

Section: Discussionmentioning

confidence: 99%

A 150 kDa Protein Derived from Bull Seminal Plasma Extended the Survival Time of Kacang Goat Sperm Stored at 5°C

Susilowati

Mustofa

Wurlina

et al. 2021

Veterinary Medicine International

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Artificial insemination has proven to be an effective method for increasing population size and genetic quality of Kacang goats. However, innovation is required to maintain the quality of Kacang goat semen in storage. This study aimed to examine the effects of supplementing the 150 kDa protein assumed as IGF-I complex derived from bull seminal plasma in skim milk-egg yolk extender on the quality of Kacang goat sperm stored at 5°C. Twelve ejaculates collected from three Kacang goats were divided into three groups. In the control group (T0), the ejaculates were extended with skim milk-egg yolk only. In the treatment groups (T1 and T2), the ejaculates were extended with skim milk-egg yolk supplemented with the IGF-I complex protein at 12 μg and 24 μg/100 mL, respectively. The extended semen was stored at 5°C, and the viability, motility, intactness of the plasma membrane, malondialdehyde concentration, and apoptotic sperm percentage were evaluated daily for five days. The results showed that the T1 was the most effective treatment for maintaining Kacang goat semen at a quality acceptable for artificial insemination over five days of storage at 5°C. However, the T0 and T2 groups retained acceptable qualities for only three days at 5°C. It could be concluded that supplementation of 12 μg of the 150 kDa protein derived from bull seminal plasma per 100 mL extender successfully extended the life span of Kacang goat sperm for five days.

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Section: Discussionmentioning

confidence: 99%

A 150 kDa Protein Derived from Bull Seminal Plasma Extended the Survival Time of Kacang Goat Sperm Stored at 5°C

Susilowati

Mustofa

Wurlina

et al. 2021

Veterinary Medicine International

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“…Biomarkers, such as various proteins and other biochemical attributes, lead an essential role in the biological systems of males and females (5,6,10,22,23,24,34), but the detailed information for its presence in seminal plasma, importance in sperm metabolism, and their relation with bull fertility is still unknown (18,21,25,39,41). It is well-known that buffalo semen is characterized by high contents of fructose, acid and alkaline phosphatase, and inorganic phosphorus but lower content of antioxidants compared to bull semen (8,9,13,15,40,43).…”

Section: Introductiomentioning

confidence: 99%

Some Biochemical Attributes in Seminal Plasma of Iraqi Buffalo Bulls and Their Relation to the Semen Quality

K. S. Musa,

T. A. Abdulkareem

2024

Iraqi J. Agric. Sci.

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This study was undertaken to figure out the relationship between some biochemical characteristics in seminal plasma with the semen quality of Iraqi buffalo bulls (Bubalus bubalis). Semen was diluted with the Tris extender and divided based on the sperm cell's individual motility into two groups. The first group included good-semen quality (GS; n=3), whereas, the second group included poor-semen quality (PS; n=5). The differences between GS and BS groups in all seminal biochemical attributes lacked significance. A negative and significant (P≤0.05) correlation coefficient was noticed between cholesterol concentration and both principal and terminal sperms abnormality (r= -0.52), and total abnormalities (r= -0.53). A positive and significant (P≤0.05) correlation coefficient was shown between cholesterol concentration and normal sperm percentage (r= 0.53). Moreover, there was a negative and significant correlation (P≤0.05) between the triglyceride concentration and each of the percentages of individual sperm motility (r = -0.50) and of live sperm (r = -0.51). It can be concluded that some biochemical characteristics of seminal plasma can be used to predict the semen quality and fertility of Iraqi buffalo bulls.

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“…Buffalo semen has an anti‐oxidative stress system that includes enzymatic and nonenzymatic antioxidants (Turaja et al., 2019 ), but during cryopreservation, due to the low concentration of these antioxidants in semen, the endogenous defence system is insufficient for countering this stress (Ansari et al., 2012 ). Low temperatures during the buffalo semen freezing and thawing cycle cause excessive adenosine triphosphate consumption, cell membrane lesions, DNA apoptosis, increased osmotic pressure, early acrosome reactions, and oxidative stress, reducing sperm motility and fertilisation capacity (De Lamirande et al., 1997 ; Tariq et al., 2015 ; Ezz et al., 2017 ). Therefore, an improvement in sperm cryopreservation leads to increased productivity in the breeding program of this animal species (Topraggaleh et al., 2014 ).…”

Section: Introductionmentioning

confidence: 99%

The influence of L‐proline and fulvic acid on oxidative stress and semen quality of buffalo bull semen following cryopreservation

Ramazani¹,

Gharebagh

Soleimanzadeh

et al. 2023

Veterinary Medicine & Sci

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Background: This study investigates the effects of cryopreservation and supplementation of Azeri water buffalo's semen with proline (Lp) and fulvic acid (FA).Objectives: Therefore, this study aimed to assess motility parameters, sperm viability, oxidative stress parameters, and DNA damage to detect the optimum concentrations of Lp and FA for buffalo semen cryopreservation.Methods: Thirty semen samples of three buffalo bulls were diluted in Tris-egg yolk extender and divided into 12 equal groups including control (

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The effect of cholesterol loaded cyclodextrins on post-thawing quality of buffalo semen in relation to sperm DNA damage and ultrastructure

Cited by 18 publications

References 44 publications

A 150 kDa Protein Derived from Bull Seminal Plasma Extended the Survival Time of Kacang Goat Sperm Stored at 5°C

A 150 kDa Protein Derived from Bull Seminal Plasma Extended the Survival Time of Kacang Goat Sperm Stored at 5°C

Some Biochemical Attributes in Seminal Plasma of Iraqi Buffalo Bulls and Their Relation to the Semen Quality

The influence of L‐proline and fulvic acid on oxidative stress and semen quality of buffalo bull semen following cryopreservation

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