The effect of chromium on rat insulinoma cells in high glucose conditions

Kwon, Min Jeong; Chung, Haegeun; Yoon, Chang Shin; Ko, Jung Hae; Jun, Hae Jung; Kim, Tae Kyoung; Lee, Soon H.; Ko, Kyung Soo; Rhee, Byung Doo; Kim, Mi K.; Park, Jeong H.

doi:10.1016/j.lfs.2010.08.001

Cited by 6 publications

(5 citation statements)

References 33 publications

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“…To establish an in vitro model for diabetes‐induced endothelial dysfunction and ED that mimics the in vivo situation, we exposed MCECs to a high‐glucose condition [36–38]. In the present study, MCECs exposed to high glucose had higher RAGE and superoxide anion levels than did MCECs exposed to normal glucose.…”

Section: Discussionmentioning

confidence: 58%

Matrigel-Based Sprouting Endothelial Cell Culture System from Mouse Corpus Cavernosum is Potentially Useful for the Study of Endothelial and Erectile Dysfunction Related to High-Glucose Exposure

Yin

Kwon

Shin

et al. 2012

The Journal of Sexual Medicine

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Introduction A proper cavernous endothelial cell culture system would be advantageous for the study of the pathophysiologic mechanisms involved in endothelial dysfunction and erectile dysfunction (ED). Aim To establish a nonenzymatic technique, which we termed the “Matrigel-based sprouting endothelial cell culture system,” for the isolation of mouse cavernous endothelial cells (MCECs) and an in vitro model that mimics in vivo situation for diabetes-induced ED. Methods For primary MCEC culture, mouse cavernous tissue was implanted into Matrigel and sprouting cells from the tissue were subcultivated. To establish an in vitro model for diabetes-induced ED, the primary cultured MCECs were exposed to a normal-glucose (5 mmoL) or a high-glucose (30 mmoL) condition for 48 hours. Main Outcome Measures The purity of isolated cells was determined by fluorescence-activated cell sorting analysis. MCECs incubated under the normal- or the high-glucose condition were used for Western blot, cyclic guanosine monophosphate (cGMP) quantification, and in vitro angiogenesis assay. Results We could consistently isolate high-purity MCECs (about 97%) with the Matrigel-based sprouting endothelial cell culture system. MCECs were subcultured up to the fifth passage and no significant changes were noted in endothelial cell morphology or purity. The phosphorylation of Akt and eNOS and the cGMP concentration were significantly lower in MCECs exposed to high glucose than in those exposed to normal glucose. MCECs exposed to the normal-glucose condition formed well-organized capillary-like structures, whereas derangements in tube formation were noted in MCECs exposed to high glucose. The protein expression of transforming growth factor-β1 (TGF-β1) and phospho-Smad2 was significantly increased by exposure to high glucose. Conclusion The Matrigel-based sprouting endothelial cell culture system is a simple, technically feasible, and reproducible technique for isolating pure cavernous endothelial cells in mice. An in vitro model for diabetic ED will be a valuable tool for evaluating the angiogenic potential of novel endogenous or synthetic modulators.

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Section: Discussionmentioning

confidence: 58%

Matrigel-Based Sprouting Endothelial Cell Culture System from Mouse Corpus Cavernosum is Potentially Useful for the Study of Endothelial and Erectile Dysfunction Related to High-Glucose Exposure

Yin

Kwon

Shin

et al. 2012

The Journal of Sexual Medicine

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“…On the other hand, some metallic species are considered important in glucose metabolism. For example, trivalent chromium was proposed as a structural part of glucose tolerance factor and even though such compound has not been confirmed, it is believed that Cr participates in stimulation of insulin signaling [21]. Other elements to be mentioned is zinc, which is necessary for the synthesis of insulin hexamer [22].…”

Section: Discussionmentioning

confidence: 99%

A Comparative Study of Relationship between Micronutrients and Gestational Diabetes

Akhlaghi

Bagheri

Rajabi

2012

ISRN Obstetrics and Gynecology

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In this paper, we studied the relation between the micronutrient and gestational diabetes. Therefore, we measured micronutrient concentration including Ni, Al, Cr, Mg, Fe, Zn, Cu, and Se in serum of women with gestational diabetes between 24 and 28 weeks of gestational age (study group) who had inclusion criteria and comparison with micronutrient levels in normal pregnant women with same gestational age (control group). Results showed that there was no significant difference between the serum micronutrient level (Ni, Al, Cr, Mg, Zn, Cu, Se) in study and control groups except serum level of iron which in serum of gestational diabetic women was lower than normal pregnant women and difference was significant.

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“…Chromium (Cr) is required for normal carbohydrate, protein and lipid metabolism and its deficiency has been implicated as one of the causes of diabetes mellitus [18-20]. Supplementations of available Cr chelates with picolinic acid (CrPic) [21,22] and histidinate (CrHis) [23] have been shown to exert beneficial effects for the management of type-2 diabetes, as reflected by a decline in insulin response.…”

Section: Introductionmentioning

confidence: 99%

Chromium picolinate and chromium histidinate protects against renal dysfunction by modulation of NF-κB pathway in high-fat diet fed and Streptozotocin-induced diabetic rats

et al. 2012

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BackgroundDiabetic nephropathy is one of major complications of diabetes mellitus. Although chromium is an essential element for carbohydrate and lipid metabolism, its effects on diabetic nephropathy are not well understood. The present study was conducted to investigate the effects of chromium picolinate (CrPic) and chromium histidinate (CrHis) on nuclear factor-kappa B (NF-κB) and nuclear factor-E2-related factor-2 (Nrf2) pathway in the rat kidney.MethodsMale Wistar rats were divided into six groups. Group I received a standard diet (8% fat) and served as a control; Group II was fed with a standard diet and received CrPic; Group III was fed with a standard diet and received CrHis; Group IV received a high fat diet (HFD, 40% fat) for 2 weeks and then were injected with streptozotocin (STZ) (HFD/STZ); Group V was treated as group IV (HFD/STZ) but supplemented with CrPic for 12 weeks. Group VI was treated as group IV (HFD/STZ) but supplemented with CrHis.ResultsThe increased NF-κβ p65 in the HFD/STZ group was inhibited by CrPic and CrHis supplementation (P < 0.05). In STZ-treated rats, a significant decrease in levels of nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (IκBα) was found in kidney tissues when compared to control rats (P < 0.05). A significant increase in the levels of IκBα was observed in CrPic- and CrHis-treated rats when compared with STZ-treated rats. Renal Nrf2 levels were significantly decreased in diabetic rats compared with the control rats. There was a higher tendency for increase of kidney Nrf2 level and decrease in kidney NFκBp65 levels and 4- hydroxyl nonenal (4-HNE) protein adducts (P < 0.05) in diabetic rats.ConclusionOur result show that in kidney tissue CrHis/CrPic increases Nrf2 level, parallelly decreases NF-κB and partially restores IκBα levels in HFD/STZ group, suggesting that CrPic and CrHis may play a role in antioxidant defense system via the Nrf2 pathway by reducing inflammation through NF-κβ p65 inhibition. Moreover, a greater reduction in NF-κB expression and greater increases in expressions of IκBα and Nrf2 in diabetic rats supplemented with CrHis than rats supplemented with CrPic suggest that CrHis has more favorable effects than CrPic.

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The effect of chromium on rat insulinoma cells in high glucose conditions

Cited by 6 publications

References 33 publications

Matrigel-Based Sprouting Endothelial Cell Culture System from Mouse Corpus Cavernosum is Potentially Useful for the Study of Endothelial and Erectile Dysfunction Related to High-Glucose Exposure

Matrigel-Based Sprouting Endothelial Cell Culture System from Mouse Corpus Cavernosum is Potentially Useful for the Study of Endothelial and Erectile Dysfunction Related to High-Glucose Exposure

A Comparative Study of Relationship between Micronutrients and Gestational Diabetes

Chromium picolinate and chromium histidinate protects against renal dysfunction by modulation of NF-κB pathway in high-fat diet fed and Streptozotocin-induced diabetic rats

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