The Effect of Coenzyme Q10 and α-Tocopherol in Skim Milk–Based Extender for Preservation of Caspian Stallion Semen in Cool Condition

Yousefian, Iman; Zare-Shahneh, Ahmad; Zhandi, Mahdi

doi:10.1016/j.jevs.2014.04.002

Cited by 33 publications

(21 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Yousefian et al. () verified that the association of CoQ10 (1 μ m ) with α ‐TOH (5 m m ) appeared more efficient in preventing LPO. This same result can be seen in this experiment in which α ‐TOH and the association of CoQ10 + α ‐TOH provided lower levels of LPO, suggesting a synergetic action among these antioxidants through the recycling of α ‐TOH in α ‐TOH (Fig.…”

Section: Discussionmentioning

confidence: 98%

“…), so better efficacy of the OXPHOS mitochondrial can bring benefits to the sperm motility (Yousefian et al. ).…”

Section: Discussionmentioning

confidence: 99%

“…Its association with the α ‐TOH was effective in the maintenance of motility, integrity and functionality of the plasma membrane and in LPO prevention (Yousefian et al. ) during equine semen cooling.…”

Section: Introductionmentioning

confidence: 99%

“…Research works try to obtain the maximum fertilizing potential of a stallion's ejaculate through strategies such as the seminal plasma removal (Barrier-Battut et al 2013), utilization of the cholesterol-loaded cyclodextrin complex (Hartwig et al 2014) and the addition of antioxidants in the cooling extender (Aurich et al 1997;Franco et al 2013;Yousefian et al 2014).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Coenzyme Q10 and α‐Tocopherol Prevent the Lipid Peroxidation of Cooled Equine Semen

Nogueira

Sampaio

Souza

et al. 2015

Reprod Domestic Animals

View full text Add to dashboard Cite

Biotechnology applied for equine semen increases the levels of reactive oxygen species and reduces the natural antioxidant defence, by both dilution and removal of seminal plasma. Therefore, the aims of this study were to evaluate the effect of adding coenzyme Q10 (CoQ10) and α-tocopherol (α-TOH) to the cooling extender, singly or in combination, on sperm parameters, and their effectiveness in preventing lipid peroxidation (LPO) of equine semen during cooling at 5°C for 72 h. Ten adult stallions of proven fertility were used, using two ejaculates each, subjecting them to the treatments with the following concentrations: α-TOH: 2 mm; CoQ10: 40 μg/ml; and CoQ10 + α-TOH: 40 μg/ml + 2 mm for control (C) without the addition of antioxidants and for vehicle control (EtOH) with 100 μl ethanol. The CoQ10 group had a higher percentage of total motility (69.1 ± 16.2%) compared to control (62.1 ± 16.2%) and EtOH (58.1 ± 18.6%). CoQ10 + α-TOH and α-TOH groups were most effective in preventing LPO compared to controls (1765.9 ± 695.9, 1890.8 ± 749.5, 2506.2 ± 769.4 ng malondialdehyde/10(8) sptz, respectively). In conclusion, CoQ10 and α-TOH were effective during the cooling process of equine semen at 5°C for 72 h, providing increased levels of total motility, as well as lower LPO.

show abstract

Section: Discussionmentioning

confidence: 98%

“…), so better efficacy of the OXPHOS mitochondrial can bring benefits to the sperm motility (Yousefian et al. ).…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Coenzyme Q10 and α‐Tocopherol Prevent the Lipid Peroxidation of Cooled Equine Semen

Nogueira

Sampaio

Souza

et al. 2015

Reprod Domestic Animals

View full text Add to dashboard Cite

show abstract

“…En un estudio donde se incluyó α-tocoferol en la dieta de burros, no se observó efecto sobre la calidad seminal (36) . En una investigación reciente donde se evaluó la adición de α-tocoferol en diluyentes para semen equino, no se encontró efecto sobre la calidad seminal (37) ; sin embargo, otros autores reportaron un efecto promisorio de esta vitamina, en el mejoramiento de la integridad y la estabilidad de la membrana plasmática (38) . En otro estudio, el tocoferol redujo la peroxidación lipídica de semen equino sometido a periodos cortos de incubación (39) .…”

Section: Resultados Y Discusiónunclassified

Supplementation with seminal plasma and relationship of its components with the quality of frozen-thawed semen of donkeys (Equus asinus)

Páez

Rojano

Betancur

2017

Rev. Mex. Cienc. Pecu.

View full text Add to dashboard Cite

El objetivo fue evaluar la suplementación con plasma seminal y la relación existente entre algunos de sus componentes, con la calidad del semen congelado-descongelado de asnos (Equus asinus). El semen de cinco asnos criollos colombianos se colectó por el método de la vagina artificial. A partir de una fracción de cada eyaculado se separó el plasma seminal por centrifugación y se evaluó su contenido de vitamina C (VC), vitamina E (VE), proteínas totales (PT) y perfil lipídico (PL). La criopreservación del semen se realizó por congelación convencional en un diluyente suplementado con 20 % de plasma seminal. Se evaluó la movilidad, la integridad estructural de membrana (IEM), la morfología anormal (MA) y la integridad funcional de membrana (HOS) de los espermatozoides, mediante el sistema computarizado SCA ® , el ensayo fluorescente SYBR14/IP, la tinción con eosina-nigrosina y la prueba hipoosmótica, respectivamente. Se ajustaron modelos mixtos y se realizó un análisis de correlación de Pearson entre los componentes del plasma seminal y la calidad espermática. Se encontró que un nivel alto de VC, VE, PT y ácido esteárico (C18:0) tuvo un efecto deletéreo sobre la calidad del semen criopreservado (P≤0.05). Mientras que un nivel bajo de PT y C18:0, y un nivel medio de VE y VC presentaron resultados post-descongelación superiores para la movilidad y la integridad de membrana (P≤0.05). Se hallaron coeficientes de correlación negativos de VC, VE, PT y C18:0 con diferentes parámetros de calidad seminal post-descongelación. Se concluye que la composición del plasma seminal suplementado para la congelación de semen de asno, influye en la calidad espermática post-descongelación. PALABRAS CLAVE: Criopreservación, Semen de asno, Lípidos, Proteínas, Vitaminas. ABSTRACTThe objective was to evaluate seminal plasma supplementation and the relationship between some of its components with frozen-thawed semen quality of donkey (Equus asinus). Semen from five Colombian Creole donkeys was collected by the artificial vagina method. From a fraction of each ejaculate the seminal plasma was separated by centrifugation, and vitamin C (VC), vitamin E (VE), total protein (TP) and lipid profile (LP) was evaluated. Semen cryopreservation was performed by conventional freezing in supplemented extender with 20 % of seminal plasma. Motility, structural membrane integrity (SMI), abnormal morphology (AM) and functional membrane integrity (HOS) of sperm, was assessed by SCA ® computerized system, SYBR14 / IP fluorescent assay, eosin-nigrosine staining and hypo osmotic swelling test, respectively. Mixed models were fitted where the fixed effect of the level of each component is included and a Pearson correlation analysis was performed between the plasma components and sperm quality. Means were compared by the Tukey test. It was found that high levels of VC, VE, TP and stearic acid (C18:0) had a deleterious effect on cryopreserved semen quality (P≤0.05). While a low level of PT and C18: 0, and a medium level of VE and VC showed higher post-thaw results...

show abstract

Effect of argan oil on liquid storage of ram semen in Tris or skim milk based extenders

Allai

Druart

Contell

et al. 2015

Animal Reproduction Science

View full text Add to dashboard Cite

The Effect of Coenzyme Q10 and α-Tocopherol in Skim Milk–Based Extender for Preservation of Caspian Stallion Semen in Cool Condition

Cited by 33 publications

References 38 publications

Coenzyme Q10 and α‐Tocopherol Prevent the Lipid Peroxidation of Cooled Equine Semen

Coenzyme Q10 and α‐Tocopherol Prevent the Lipid Peroxidation of Cooled Equine Semen

Supplementation with seminal plasma and relationship of its components with the quality of frozen-thawed semen of donkeys (Equus asinus)

Effect of argan oil on liquid storage of ram semen in Tris or skim milk based extenders

Contact Info

Product

Resources

About