The Effect of Cold Aqueous Extract and some Proteinous Compounds from Cyperus rotundus L. Tubers in the Level of Glucose and Cholesterol in Mice

Abstract

Two proteinous compounds (I and II) had been isolated by gel filtration chromatography of the full saturated precipitate produced by ammonium sulphate of the aqueous extract of the tubers. The apparent molecular weights of the isolated components(I and II) were in the rang of (63096, 5012) dalton using gel filtration technique and in the range of (61000, 5000) dalton using sodium dodecyl sulphatepolyacrylamide gel electrophoresis technique.The results showed that the aqueous extract and the low molecular weight proteinous compound (II) were lowered glucose level using a dose of 75 mg/kg body weight and the percent decrease were 45% and 47% respectively in normal mice. Where as in diabetic mice, the low molecular weight proteinous compound (II) was the most effective in lowering glucose level using both doses (75 and 125 mg/kg body weight) and the percent decrease were 64% and 69% respectively.Finally, the aqueous extract and the proteinous compound I (125 mg/kg body weight) were the most effective in lowering cholesterol level in normal mice. Where as in diabetic mice, the aqueous extract and the proteinous compound I in both doses (125 and 75 mg/kg body weight) were the most effective in lowering cholesterol level.