The Effect of Commercially Available Endodontic Cements and Biomaterials on Osteogenic Differentiation of Dental Pulp Pluripotent-Like Stem Cells

Abstract

The aim of this study is to compare the osteogenic differentiation capacity of the dental pulp pluripotent-like stem cells (DPPSCs) using conditional media pretreated with ProRoot-MTA, Biodentine (BD) or the newly manufactured pure Portland cement Med-PZ (MZ). DPPSCs, isolated from human third molars, are the most relevant cell model to draw conclusions about the role of biomaterials on dental tissue regeneration. Cytotoxicity, alkaline phosphatase (ALP) activity, and calcium deposition analysis were evaluated at different differentiation time points. Gene expression of key osteogenic markers (RUNX2, Collagen I and Osteocalcin) was determined by qRT-PCR analysis. The osteogenic capacity of cells cultured in conditioned media prepared from MZ or MTA cements was comparable. BD conditioned media supported cell proliferation but failed to induce osteogenesis. Relative to controls and other cements, high osteogenic gene expression was observed in cultures pre-treated with the novel endodontic cement MZ. In conclusion, the in vitro behavior of a MZ- endodontic cement was evaluated, showing similar enhanced cell proliferation compared to other commercially available cements but with an enhanced osteogenic capacity with prospective potential as a novel cement for endodontic treatments.

Bioactivity of Bioceramic Materials Used in the Dentin-Pulp Complex Therapy: A Systematic Review

“…Cytotoxicity and biocompatibility of a wide range of bioceramic materials towards dental stem cells (DSCs) have been investigated in numerous studies [13,14,15,16,17]; among others. The well-known Pro-Root MTA (Dentsply Tulsa Dental Specialties, Tulsa, OK, USA) has been shown to increase osteoblast, fibroblast, cementoblast, odontoblast and pulp cell differentiation, but its handling difficulty among other limitations encourages for a search for alternative materials [13].…”

“…Cytotoxicity and biocompatibility of a wide range of bioceramic materials towards dental stem cells (DSCs) have been investigated in numerous studies [13,14,15,16,17]; among others. The well-known Pro-Root MTA (Dentsply Tulsa Dental Specialties, Tulsa, OK, USA) has been shown to increase osteoblast, fibroblast, cementoblast, odontoblast and pulp cell differentiation, but its handling difficulty among other limitations encourages for a search for alternative materials [13]. Materials like Biodentine (Septodont, Saint Maurdes-Fosses, France) and TheraCal LC (Bisco Inc., Schaumburg, IL, USA) are examples of bioceramic materials introduced posteriorly in dentistry for their use in vital pulp therapy as blood clot protectors in pulpal revascularization procedures, standing out for their consistency, easier manipulation and tricalcium silicate composition [16].…”

Chemically Defined Conditions Mediate an Efficient Induction of Dental Pulp Pluripotent-Like Stem Cells into Hepatocyte-Like Cells

“…First-strand cDNA was synthesized from 200 ng RNA by reverse transcription using QuantiTect Reverse Transcription Kit (Qiagen Inc., Germantown, MD, USA). qRT-PCR reactions were performed as previously described [ 43 , 44 ]. Primer pairs with equivalent efficiencies ( Table 1 ) were selected from PrimerBank [ 45 ] or designed using Primer-BLAST tools ( http://www.ncbi.nlm.nih.gov/tools/primer-blast/ ) [ 46 ].…”

Role and application of stem cells in dental regeneration: A comprehensive overview

“…The highlights of recent researches on the oral stem cells are summarized in Table 1 (Tab. 1) (References in Table 1: Chrepa et al, 2017[ 17 ]; De Berdt et al, 2015[ 22 ]; Farhad Mollashahi et al, 2016[ 27 ]; Ferro et al, 2011[ 31 ]; Gao et al, 2013[ 32 ]; Ginani et al, 2018[ 34 ]; Gu and Shi, 2016[ 37 ]; Gu et al, 2013[ 36 ]; Huang et al, 2017[ 46 ]; Iwasaki et al, 2014[ 49 ]; Jeong et al, 2013[ 52 ]; Kanafi et al, 2013[ 55 ]; Kim et al, 2013[ 58 ]; Kim et al, 2015[ 57 ]; Kim et al, 2017[ 56 ]; Lertchirakarn and Aguilar, 2017[ 63 ]; Li et al, 2017[ 65 ]; Liu et al, 2018[ 68 ]; Maher et al, 2018[ 73 ]; Maimets et al, 2015[ 75 ]; Martinez-Sarra et al, 2017[ 78 ]; Nicola et al, 2017[ 92 ]; Nunez-Toldra et al, 2017[ 96 ], 2019[ 97 ]; Peng et al, 2017[ 108 ]; Pringle et al, 2016[ 113 ]; Regmi et al, 2017[ 114 ]; Rossato et al, 2017[ 117 ]; Sanches et al, 2018[ 119 ]; Tsai et al, 2015[ 129 ]; Ustiashvili et al, 2014[ 133 ]; Xu et al, 2017[ 139 ]; Yuan et al, 2015[ 143 ]; Zhang et al, 2009[ 149 ], 2017[ 152 ], 2019[ 151 ]).…”