The Effect of Conditioned Media of Adipose-Derived Stem Cells on Wound Healing after Ablative Fractional Carbon Dioxide Laser Resurfacing

Abstract

Objective. To evaluate the benefits of conditioned medium of Adipose-derived stem cells (ADSC-CM) on wound healing after fractional carbon dioxide laser resurfacing (FxCR) on human skin. Materials and Methods. Nineteen subjects were treated with FxCR on the bilateral inner arms. ADSC-CM was applied on FxCR site of one randomly selected arm. Transepidermal water loss (TEWL), skin color, and gross-elasticity of FxCR site on both arms were measured. Skin samples were taken by biopsy from three subjects 3 weeks after treatment for histopathological manifestations and mRNA expressions of procollagen types I and III, elastin genes were noted. Results. The index of erythema, melanin, and TEWL of the ADSC-CM-treated skin were significantly lower than those of the control side. The mRNA expression of type III procollagen in ADSC-CM-treated group at 3 weeks posttreatment was 2.6 times of that of the control group. Conclusion. Application of allograft ADSC-CM is an effective method for enhancing wound healing after FxCR, by reducing transient adverse effects such as erythema, hyperpigmentation, and increased TEWL.

Insights into cell‐free therapeutic approach: Role of stem cell “soup‐ernatant”

“…Serum from the patient undergoing therapy should be used for culturing SCs and subsequent secretome harvesting. Although clinical trials using conditioned medium have not been reported yet, recently two pilot studies regarding usage of CM from ASCs have shown promise in regeneration of hair follicle and ablative fractional carbon dioxide resurfacing wound healing in humans . Current studies mainly focus on the secretory and regulatory factors of SCs holding great potential in lieu of forthcoming studies in the SC field.…”

“…There have been very few clinical studies on therapeutic trials of SC secretome in human subjects. These involve testing of wound healing potential , an increased hair regeneration in human females after secretome treatment as compared with placebo , and a recent study in human patients for alveolar bone regeneration . In these studies, no inflammation or any other local or systemic complications were reported following secretome treatment, which further reflected the safe use of secretome therapy in clinical trials.…”

Mesenchymal stem cell‐conditioned media: A novel alternative of stem cell therapy for quality wound healing

“…Various studies have used different methods to assess various cytokines in the conditioned media (CM), from the conventional enzyme‐linked immunosorbent assay (ELISA; Bhang et al, 2014) to proteomic profiling methods (Ho, Lai, & Li, 2012). Very limited data are available regarding the use of CM in regenerative therapy like hair follicle regeneration (Fukuoka, Suga, Narita, Watanabe, & Shintani, 2012) and wound healing (Ansari et al, 2013; Zhou et al, 2013). The regenerative effects of lyophilized MSCs‐CM on wound healing have not been fully explored in vivo.…”

A Single Arm, Monocentric, Prospective, Open Label Study to Evaluate Anti-Ageing Efficacy of Human Growth Factors and Cytokines Containing Cosmetic Skin Serum in Indian Female Population

“…Increase in epidermal thickness and increased fibroblast density in dermis in addition to clinical benefits of wrinkle reduction was seen in a study by Hussain et al [24]. In another study, application of adipose derived stem cells conditioned medium increased the synthesis of type I and III procollagen and elastin genes [9]. However, the study failed to show clinical efficacy, probably due to one-time administration of conditioned medium.…”