The Effect of Copper And Zinc Nanoparticles on the Growth Parameters, Contents of Ascorbic Acid, and Qualitative Composition of Amino Acids and Acylcarnitines in Pistia stratiotes L. (Araceae)

Abstract

The paper covers the research of copper and zinc nanoparticle effect on the content of ascorbic acid, and quantitative and qualitative composition of amino acids and acylcarnitines in Pistia stratiotes L. plants. Plant exposition to copper nanoparticles led to the decrease in (1) the amount of ascorbic acid, (2) the total content of amino acids (by 25 %), and (3) the amount of all studied amino acids except for the glycine amino acid. At this, the amount of 5-oxoproline, arginine, leucine, ornithine, phenylalanine, proline, serine, and tyrosine was two times lower than in control plants. The reduction of the contents of 8 out of 12 investigated acylcarnitines (namely C0, C2, C3, C5, C6, C8, C16, C18:1) was observed in plants under the influence of copper nanoparticles. The result of plants incubation with zinc nanoparticles was the decrease in (1) the amount of ascorbic acid, (2) the total content of amino acids (by 15 %), (3) the content of leucine, methionine, phenylalanine, proline, and tyrosine (more than twice), and (4) the content of 10 acylcarnitines (C0, C2, C3, C4, C5, C10, C16, C18, C18:1, C18:2). The observed reduction in amino acid contents may negatively affect plants adaptive reactions associated with de novo synthesis of stress proteins. At the same time, the decrease in the content of acylcarnitines, responsible for fatty acid transportation, may lead to the changes in the activity and direction of lipid metabolism in plants and reduce plant's ability to use free fatty acids as the oxidation substrate for cell reparation.

Copper Ions Induce DNA Sequence Variation in Zygotic Embryo Culture-Derived Barley Regenerants

“…The regenerants were morphologically identical to the donor. This was unexpected since copper nanoparticles (to a lesser extent than in the presence of zinc nanoparticles) have been shown to affect chlorophyll-a in Pistia stratiotes L. (Araceae), leading to morphological variation ( Olkhovych et al, 2016 ). It should be noted that copper and zinc function together to induce oxidative stress ( Lee et al, 2002 ).…”

Interaction of Copper-Based Nanoparticles to Soil, Terrestrial, and Aquatic Systems: Critical Review of the State of the Science and Future Perspectives

“…Morphological changes in the root system were more prominent. In comparison to the control plant, blackening of roots together with inhibition of new growth roots, and a decrease in plant weight, amino acids, and the content of ascorbic acid reduced by 63% was observed in exposed plants (Olkhovych et al 2016). The morphological changes were also observed for plant L. gibba in the form of leaf reduction and detachment of fronds from the plant.…”

Insight into the biochemical and physiological mechanisms of nanoparticles-induced arsenic tolerance in bamboo

“…Consequently, these two molecules can form an efficient redox system that protects plant cells from ROS under stressful conditions (Yin et al, 2022). Additionally, AsA can boost plant photochemical activity through promoting photosynthesis (Olkhovych et al, 2016). The high concentration of AsA in the plant could be a positive indicator of the plant's defense system capacity to respond to environmental stress.…”