Wine and tourism represent a perfect symbiosis, as they offer a different experience to tourists and also promote the economic, social, and environmental development of wine regions. In Spain, wine tourism is an increasingly important research field and several studies have been conducted on the role of wine routes in boosting the competitiveness of a territory, increasing wine production, improving the quality of life of citizens, and respecting the environment. However, to the best of our knowledge, the economic impact generated by these routes in Spain has not been addressed in the academic literature. To overcome this research gap, this paper aims to analyze the supply and demand of tourism activities by examining the evolution of the institutions involved in the Spanish wine routes, on the one hand, and the economic impact of these routes, on the other. The results show a sharp drop in the supply of institutions and the demand for tourist routes in 2020 as a result of COVID-19, with a decrease of 2.58% in the total number of institutions adhered to the wine routes, a decrease of 73.53% in the total number of visitors to the different Wine Routes of Spain and a decrease of 74.7% in the economic impact compared to the previous year. Likewise, the research shows the existence of differences between the Wine Routes of Spain in terms of the number of visitors and their economic impact, which could serve as a guide for managers to make investments through acquisitions and/or their own investments in wineries located on the routes with the greatest economic impact..