The effect of dentine‐bonding agents on substance P release in human dental pulp

Abstract

Dentine-bonding agents placed over Class V cavity preparations increased SP release. One-step dentine-bonding agents increased SP release most.

The Effect of Three Different Rotary Instrumentation Systems on Substance P and Calcitonin Gene-related Peptide Expression in Human Periodontal Ligament

“…Neuropeptides were extracted by adding 150 mL of 0.5 mol/L of acetic acid and double boiling in a thermostat bath for 30 minutes in accordance with previously reported protocols (19)(20)(21)(22). SP and CGRP expression were determined by competition binding assays using human SP and human CGRP-radioimmunoassay (RIA) kits (RK-061-05 and RK-015-02; Phoenix Peptide Pharmaceutical, Belmont, CA).…”

The Effect of Orthodontic Forces on Calcitonin Gene-related Peptide Expression in Human Dental Pulp

“…Neuropeptide was extracted by adding 150 mL of 0.5 mol/L acetic acid and double boiling in a thermostat bath for 30 minutes in accordance with previously reported protocols (11)(12)(13)(14)(15)(16).…”

Effect of Experimentally Induced Occlusal Trauma on Substance P Expression in Human Dental Pulp and Periodontal Ligament

“…Neuropeptides were extracted by adding 150 mL 0.5 mol/L À1 acetic acid and double-boiling in a thermostat bath for 30 minutes in accordance with previously reported protocols (11,12,(16)(17)(18).…”

“…In dental pulp, it has been reported that its expression increases with carious progression (15) and with restorative procedures such as tooth bleaching (16), cavity preparation (17), and dentin bonding (18). SP has also been implicated during periodontal ligament inflammatory process (13,19,20).…”