The Effect of Orthodontic Forces on Calcitonin Gene-related Peptide Expression in Human Dental Pulp

Caviedes-Bucheli, Javier; Monsalve, Jaime Omar Moreno; Ardila-Pinto, Jacqueline; Toro-Carreño, Hilda Rosa Del; Saltarín-Quintero, Hernando; Sierra-Tapias, Claudia Liliana; Macías-Gómez, Fabio; Ulate, Esteban; Lombana-Sanchez, Nelson; Muñoz, Hugo Roberto

doi:10.1016/j.joen.2011.03.035

Cited by 31 publications

(43 citation statements)

References 32 publications

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“…Such an increase was noted when irreversible pulpitis was diagnosed 17,26 after 10 minutes of induced pulp inflammation 27 and after orthodontic stimulation. 29 When considered together, it must be remembered that both are known to be collocated within the same sensory nonmyelinated fiber, a fact corroborated by evidence that demonstrated that an increased density of both nerve-containing fibers is found in the dental pulp of mechanically stressed teeth. 30 The presence of these two factors in pulp tissue may not be surprising, as 87% of the axons that enter through the apical region of a premolar are C nonmyelinated fibres.…”

Section: Discussionmentioning

confidence: 97%

Expression of substance P, calcitonin gene-related peptide, β-endorphin and methionine-enkephalin in human dental pulp tissue after orthodontic intrusion: A pilot study

Chavarría‐Bolaños

Martinez-Zumaran

Lombana³

et al. 2013

The Angle Orthodontist

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Objective: To determine the levels of two sensory neuropeptides (substance P [SP] and calcitonin gene-related peptide [CGRP]) and two endogenous opioids (methionine-enkephalin [Met-Enk] and bendorphin [b-End]) in dental pulp tissue samples subjected to controlled orthodontic intrusive forces. Materials and Methods: Sixteen healthy premolars were selected from eight patients who were undergoing extraction for orthodontic purposes. Eight were randomly used as controls, and the other eight were assigned to an experimental group (controlled orthodontic intrusive forces applied for 24 hours). After this period, teeth were extracted, and pulp samples were obtained. All samples were processed to quantify the expression levels of SP, CGRP, Met-Enk, and b-End using commercial radioimmunoassay kits. Results: All samples exhibited basal levels of both neuropeptides and endogenous opioids. After 24 hours of the intrusive stimulus, all patients reported a tolerable discomfort localized at the involved premolar. Only SP was significantly increased (P , .05). For the other molecules, no statistically significant differences were observed (P . .05); however, they expressed important increasing trends. Conclusions: The expression levels of SP and CGRP in dental pulp samples from the experimental group support the positive correlation between the symptomatic clinical scenario and increased expression levels of neuropeptides, clarifying the role of neurogenic inflammation in early injury response. (Angle Orthod. 2014;84:521-526.)

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Section: Discussionmentioning

confidence: 97%

Expression of substance P, calcitonin gene-related peptide, β-endorphin and methionine-enkephalin in human dental pulp tissue after orthodontic intrusion: A pilot study

Chavarría‐Bolaños

Martinez-Zumaran

Lombana³

et al. 2013

The Angle Orthodontist

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“…Interestingly, increased immunoreactivity for TRPV1 [62, 79, 80] and CGRP [56, 81] has been previously shown in uninjured primary afferent neuronal perikarya in different models of nerve lesion. Moreover, CGRP expression may increase in several pathological conditions, including partial nerve injury, where pain hypersensitivity occurs [82–86]. …”

Section: Discussionmentioning

confidence: 99%

Bortezomib Treatment Produces Nocifensive Behavior and Changes in the Expression of TRPV1, CGRP, and Substance P in the Rat DRG, Spinal Cord, and Sciatic Nerve

Quartu

Carozzi

Dorsey

et al. 2014

BioMed Research International

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To investigate neurochemical changes associated with bortezomib-induced painful peripheral neuropathy (PN), we examined the effects of a single-dose intravenous administration of bortezomib and a well-established “chronic” schedule in a rat model of bortezomib-induced PN. The TRPV1 channel and sensory neuropeptides CGRP and substance P (SP) were studied in L4-L5 dorsal root ganglia (DRGs), spinal cord, and sciatic nerve. Behavioral measures, performed at the end of the chronic bortezomib treatment, confirmed a reduction of mechanical nociceptive threshold, whereas no difference occurred in thermal withdrawal latency. Western blot analysis showed a relative increase of TRPV1 in DRG and spinal cord after both acute and chronic bortezomib administration. Reverse transcriptase-polymerase chain reaction revealed a decrease of TRPV1 and CGRP mRNA relative levels after chronic treatment. Immunohistochemistry showed that in the DRGs, TRPV1-, CGRP-, and SP-immunoreactive neurons were mostly small- and medium-sized and the proportion of TRPV1- and CGRP-labeled neurons increased after treatment. A bortezomib-induced increase in density of TRPV1- and CGRP-immunoreactive innervation in the dorsal horn was also observed. Our findings show that bortezomib-treatment selectively affects subsets of DRG neurons likely involved in the processing of nociceptive stimuli and that neurochemical changes may contribute to development and persistence of pain in bortezomib-induced PN.

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“…, Caviedes‐Bucheli et al . , ). This response is necessary for pulp defence and repair to take place in cases such as pulpitis, degenerative pulp calcification and necrosis.…”

Section: Introductionmentioning

confidence: 99%

“…Although it has been demonstrated that SP is released when thermal, chemical or mechanical stimuli such as occlusal trauma, masticatory function and when orthodontics movements are applied to the tooth (Caviedes‐Bucheli et al . , , ,b, Javed et al . ), little is known about its relationship with angiogenesis as a response of pulp tissue to occlusal trauma.…”

Section: Introductionmentioning

confidence: 99%

Angiogenic mechanisms of human dental pulp and their relationship with substance P expression in response to occlusal trauma

et al. 2016

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The Effect of Orthodontic Forces on Calcitonin Gene-related Peptide Expression in Human Dental Pulp

Cited by 31 publications

References 32 publications

Expression of substance P, calcitonin gene-related peptide, β-endorphin and methionine-enkephalin in human dental pulp tissue after orthodontic intrusion: A pilot study

Expression of substance P, calcitonin gene-related peptide, β-endorphin and methionine-enkephalin in human dental pulp tissue after orthodontic intrusion: A pilot study

Bortezomib Treatment Produces Nocifensive Behavior and Changes in the Expression of TRPV1, CGRP, and Substance P in the Rat DRG, Spinal Cord, and Sciatic Nerve

Angiogenic mechanisms of human dental pulp and their relationship with substance P expression in response to occlusal trauma

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