The effect of diclofenac on proliferation and production of growth factors by endothelial cells (HMEC-1) under hypoxia and inflammatory conditions

Wiktorowska-Owczarek, Anna

doi:10.2478/acph-2014-0006

Cited by 15 publications

(11 citation statements)

References 18 publications

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“…These results are in a close agreement with those of Flis et al [7] who demonstrate that sulindac sulide (non-selective COX-2 inhibitor) and celecoxib (selective COX-2 inhibitor) inhibit the survival of hMEC-1 cells and induce their apoptosis. A similar effect is shown by Niederburger et al (11) in human umbilical vein endothelial cells (macrovessels-derived endothelial cells) and in our previous studies (15,16,18). It was also investigated whether NS-398 could modulate bFGF secretion.…”

Section: Resultssupporting

confidence: 91%

“…These observation can be explained according to Fairweather et al (6), who report that reepithelialization and wound closure are delayed by celecoxib (selective COX-2 inhibitor) treatment. The observations presented in this paper and others (7,11,16,18) may indicate that very popular OTC medicines, for instance common use of pain killers (diclofenac, ibuprofen) in wounds or in cancers, prolong wound healing.…”

Section: Resultssupporting

confidence: 56%

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The influence of selective COX-2 inhibitor on phase of healing surgical wounds: proliferation and secretion of bFGF by endothelial cells

Jasiak

Kowalczyk

Mazan

et al. 2018

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<p>The process of wound healing consists of the following phases: inflammation, proliferation, remodeling. Non-steroidal antiinflammatory drugs may be important in this process, especially in a stage called angiogenesis. For this reason, it was decided to investigate the effect of selective COX-2 (cyclooxygenase 2) inhibitor (NS-398) on the proliferation of endothelial cells and their ability to secrete bFGF (fibroblast growth factor) for vascular endothelial cells (HMEC-1). For determination of the secretion of bFGF in a cell line HMEC-1 immunosorbent ELISA assays were used. In turn, the cell proliferation assay was performed using the MTT method. Using MTT method, it was found that NS-398 at 10 μM did not affect cell viability. Whereas selective COX-2 inhibitor at 100 μM decreased cell viability in a statistically significant manner and inhibited the proliferative effect of 100 μg/mL LPS at concentrations of 10 and 100 μM. In the further step, application of NS-398 (10 and 100 μM) with LPS (100 μg/mL; inflammatory environment) reduced the secretion of bFGF in a statistically significant manner. The investigations showed that NS-398 has an antiangiogenic effect which is based on reducing the proliferation of vascular endothelial cells and inhibiting the secretion of bFGF- factor responsible for angiogenesis during wound healing.</p>

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Section: Resultssupporting

confidence: 91%

Section: Resultssupporting

confidence: 56%

The influence of selective COX-2 inhibitor on phase of healing surgical wounds: proliferation and secretion of bFGF by endothelial cells

Jasiak

Kowalczyk

Mazan

et al. 2018

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“…Cells were harvested every third day in a trypsin-EDTA solution (0.25% trypsin, 1 mM EDTA). HMEC-1 cells were cultured according to the method described in the literature [22], and the author’s own modification [23]. …”

Section: Methodsmentioning

confidence: 99%

Abies Concolor Seeds and Cones as New Source of Essential Oils—Composition and Biological Activity

et al. 2017

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The chemical composition, including the enantiomeric excess of the main terpenes, of essential oils from seeds and cones of Abies concolor was studied by chromatographic (GC) and spectroscopic methods (mass spectrometry, nuclear magnetic resonance), leading to the determination of 98 compounds. Essential oils were mainly composed of monoterpene hydrocarbons. The dominant volatiles of seed essential oil were: limonene (47 g/100 g, almost pure levorotary form) and α-pinene (40 g/100 g), while α-pinene (58 g/100 g), sabinene (11 g/100 g), and β-pinene (4.5 g/100 g) were the predominant components of the cone oil. The seed and cone essential oils exhibited mild antibacterial activity, and the MIC ranged from 26 to 30 μL/mL against all of the tested bacterial standard strains: Staphylococcus aureus, Enterococcus faecalis, Enterococcus faecium, Escherichia coli, and Klebsiella pneumoniae. The cytotoxic studies have demonstrated that tested essential oils were cytotoxic to human skin fibroblasts and human microvascular endothelial cells at concentrations much lower than the MIC. The essential oils from A. concolor seeds and cones had no toxic effect on human skin fibroblasts and human microvascular endothelial cells, when added to the cells at a low concentration (0–0.075 μL/mL) and (0–1.0 μL/mL), respectively, and cultured for 24 h.

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“…Cells were harvested every third day in a trypsin-EDTA solution (0.25% trypsin, 1 mM EDTA). HMEC-1 cells were cultured according to the method described in the literature [35,36] and the authors’ own modifications. T98G glioma cell line was purchased from American Type Culture Collection (ATCC).…”

Section: Methodsmentioning

confidence: 99%

The Biological Activities of Cinnamon, Geranium and Lavender Essential Oils

et al. 2014

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Acinetobacter sp. represent an important cause of nosocomial infections. Their resistance to some antibiotics, their ability to survive on inanimate surfaces in the hospital environment and their ability to produce biofilms contributes to their virulence. The aim of the study was to determine the antibacterial properties of cinnamon, lavender and geranium essential oils against bacteria of the genus Acinetobacter isolated from several clinical materials and from the hospital environment. A comprehensive evaluation of the susceptibility of Acinetobacter sp. clinical strains to recommended antibiotics was performed. The constituents of cinnamon, lavender and geranium essential oils were identified by GC-FID-MS analysis, and their Minimal Inhibitory Concentrations (MICs) against tested clinical strains were determined by the micro-dilution broth method. In addition, the effects of essential oils on the viability of human microvascular endothelial cells (HMEC-1) and glioblastoma cell line (T98G) were evaluated. Cinnamon bark oil was the most active against clinical and environmental strains of Acinetobacter baumannii with MIC values OPEN ACCESSMolecules 2014, 19 20930 ranging from 0.5 to 2.5 µL/mL. The MIC values for geranium oil were between 7.5 and 9.5 µL/mL, and between 10.5 and 13.0 µL/mL for lavender oil. These essential oils can be best employed in the fight against infections caused by bacteria from Acinetobacter genus as components of formulations for hygiene and disinfection of hospital environment.

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The effect of diclofenac on proliferation and production of growth factors by endothelial cells (HMEC-1) under hypoxia and inflammatory conditions

Cited by 15 publications

References 18 publications

The influence of selective COX-2 inhibitor on phase of healing surgical wounds: proliferation and secretion of bFGF by endothelial cells

The influence of selective COX-2 inhibitor on phase of healing surgical wounds: proliferation and secretion of bFGF by endothelial cells

Abies Concolor Seeds and Cones as New Source of Essential Oils—Composition and Biological Activity

The Biological Activities of Cinnamon, Geranium and Lavender Essential Oils

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