The effect of dietary Bacillus subtilis supplementation on the growth performance, blood profile, nutrient retention, and caecal microflora in broiler chickens

Abstract

The objective of this study was to evaluate the effects of the B. subtilis RX7 and B. subtilis C14 on the growth performance, blood profiles, nutrient retention, and caecal microflora of broiler chickens. A total of 288, 1-d-old Ross 308 male broilers were assigned to one of four dietary treatment groups for 35 days; the negative control (NC; basal diet with no antibiotic or B. subtilis), positive control (PC; NC + 40 ppm avilamycin), T1 (NC + 0.1% B. subtilis RX7 1.0×109 cfu/g), and T2 (NC + 0.1% B. subtilis C14 1.0×109 cfu/ g). B. subtilis supplementation led to significantly higher weight gain than that in the NC. The serum haptoglobin concentration was lower in the Bacillus groups than in the NC and PC groups. The energy retention of broilers fed B. subtilis was significantly higher than that of the NC and PC broilers. The numbers of caecal Lactobacillus or Salmonella in the Bacillus groups were higher and lower, respectively, than those in the numbers in the NC and PC. The results showed that B. subtilis RX7 and C14 increased the weight gain, energy retention, and caecal Lactobacillus numbers and decreased serum haptoglobin levels and caecal Salmonella numbers in broiler chickens.

Supplementation of Lactic Acid Bacteria Derived from Ensiled Kumpai Tembaga on Live Body Weight, Gastrointestinal Tract, Internal Organs, and Blood Profiles in Pegagan Ducks

“…According to the concentration level of LAB, a notable effect (p<0.05) on body weight occurred when ducks were administered LAB starting at 10 7 cfu/ml and above compared to control treatment. The heightened body weight in this study was in line with the other studies (Shokryazdan et al, 2017;Abdel-Hafeez et al, 2017;Park et al, 2018), who stated that supplementation of probiotics was able to increase body weight gain and gain a greater body weight compared to the non-probiotic treatment in the whole experiment. These findings are also in agreement with Mohammadi Gheisar et al 2016and Lan et al (2017) that dietary LAB probiotics containing Enterococcus faecium were able to improve the live body weight of chickens compared with the control treatment.…”

“…The unmarked hematological parameters in this study are in line with other studies related to probiotic supplementation. The numbers of RBC and WBC in birds was reported not to be significantly increased by the administration of various probiotic strains, such as Bacillus subtilis RX7 and B2A (Park and Kim, 2014), E. faecium (Lan et al, 2017), and B. subtilis RX7 and C14 (Park et al, 2018), with the amounts of 2.00-2.01 (10 6 /µL) and 27.7-28.5 (10 3 /µL), 2.11-2.46 (10 6 /µL) and 19.9-20.8 (10 3 /µL), and 2.17-2.22 (10 6 /µL) and 29.2-31.0 (10 3 /µL), respectively. Those RBC and WBC values appear to be lower than that of this study, namely 4.20-4.50 (10 6 / µL) and 26.04-29.00 (10 3 /µL).…”

“…S ince the use of dietary antibiotics as antimicrobial growth promoters (AGPs) has negative impacts on animal and human health (Ghasemi-Sadabadi et al, 2019), many countries across the world have strictly prohibited the use of these antibiotics in the poultry industry . These health threats arise due to the increased resistance of pathogenic bacteria to antibiotics and the accumulation of antibiotic residues in poultry products (Park et al, 2018). This difficult situation encouraged studies to discover new alternative additives and eventually emerged probiotics as antibiotic replacements (Chen et al, 2013;Çalik et al, 2017).…”

Effect of the use of probiotic Bacillus subtilis (QST 713) as a growth promoter in broilers: an alternative to bacitracin methylene disalicylate

“…Our results agree with previous reports showing that probiotics do not induce significant changes in the hematological values of the broilers. ( Park and Kim 2014 ; Gutierrez and Corredor 2017 ; Park et al., 2018 ). Nevertheless, normal blood counts do not exclude the possibility of altered immune status or stress induced by environmental factors ( Avilez et al., 2014 ; Diaz et al., 2016 ).…”

Effects of Bacillus subtilis on Production Performance, Bone Physiological Property, and Hematology Indexes in Laying Hens

“…Several studies have reported that Bacillus subtilis alone or combined with other bacteria can be used as growth promotors or immunomodulatory factors to increase energy retention, weight gain, and feed conversion in broiler chickens [20,21]. One of the mechanisms of its effects is to enhance feed digestion and nutrient resorption in the gastrointestinal tract (GIT) [22,23], leading to positive effects on preventing bone mass loss [24] by improving bone density and mineral content such as Ca and phosphorus (P) [24][25][26].…”