The effect of different carbon sources on phenotypic expression by Fusarium graminearum strains

Abstract

Two Fusarium graminearum strains were cultured in glucose yeast extract peptone broth or minimal medium broth to measure the production of mycelial biomass, pH, mycotoxins, and aurofusarin pigment, when limited to single carbon sources (at 1%), including xylan, cellulose, starch, or glucose. A random complete block design with factorial arrangement and analysis of variance at a significance level of 0.01 were employed to test for treatment differences. Overall, the F. graminearum strains produced significantly more biomass, deoxynivalenol, and aurofusarin with xylan than with cellulose. No significant differences were found in terms of 15-acetyldeoxynivalenol production from the four carbon sources. The presence of significant interactions between the strains, carbon sources, and media led to the following specific differences. In yeast extract peptone broth, R-9828 strain yielded significantly more deoxynivalenol production with xylan than cellulose and R-9832 produced significantly more mycelium (biomass) with xylan than cellulose. R-9828 strain yielded significantly more deoxynivalenol production than the R-9832 strain. Also in yeast extract peptone broth, cellulose led to significantly higher pH values than other carbons, which might be due to the limited ability of the Fusarium strains to utilize cellulose as an energy source. Aurofusarin was the only expressed analyte to show a significant difference in minimal medium broth, and R-9832 produced significantly more aurofusarin with xylan than with cellulose in the broth. These results suggest that xylan may induce Fusarium growth and deoxynivalenol production to assist the infection process and may support the theory that F. graminearum invades through xylan in the cell walls of cereals.

Aflatoxins, fumonisins, and trichothecenes: a convergence of knowledge

“…Trichothecene production by F. graminearum is supported by a variety of carbon sources (Miller et al, 1983;Miller & Greenhalgh, 1985;Jiao et al, 2008;Zhang & Wolf-Hall, 2010). Clear differences exist in responses to a particular carbon source, and the specific effects are influenced by the nitrogen source and pH.…”

Shotgun Analysis of the Secretome of Fusarium graminearum

“…The fungus was inoculated and grown on minimal medium [10]. Cultures were constantly shaken (200 rpm) during their incubation period at 26°C until the exponential growth phase was reached.…”

Advances in Understanding Fusarium graminearum: Genes Involved in the Regulation of Sexual Development, Pathogenesis, and Deoxynivalenol Biosynthesis

“…When the velvet complex is disrupted, it leads to a notable decrease in the production of DON [143,144]. The studies have revealed that sucrose is more effective at stimulating trichothecene production compared to glucose [145,146]. Polyamine biosynthesis is crucial for both plants and their pathogens, as it plays a significant role in enhancing stress tolerance and pathogenicity [147].…”