The effect of different suturing materials for abdominal fascia wound closure on the collagen I/III expression ratio in rats

Abstract

Background Incisional hernia is a frequent complication of abdominal wall incision and has a high rate of recurrence. Most of the studies stated that non-absorbable sutures decreased incisional hernia incidences, but some stated otherwise. We aimed to compare the collagen type I/III ratio between monofilament non-absorbable sutures and multifilament absorbable sutures for abdominal fascia closure in Wistar albino rats. Methods Forty rats were divided into four groups. Groups 1 and 3 were sutured with monofilament non-absorbable ( polyvinylidene fluoride ). Groups 2 and 4 were sutured with multifilament absorbable ( polyglycolide ). Then, groups 1 and 2 were euthanized on day 4 (POD 4), while groups 3 and 4 were euthanized on day 7 (POD 7). Samples of fascia (1 × 0.5 cm) were taken for analysis. Collagen I/III ratios were measured using immunohistochemistry staining methods. Results While the expression of collagen I was not significantly different between monofilament non-absorbable and multifilament absorbable at POD 4 and 7 ( p = 0.45 and 0.81, respectively), the expression of collagen III reached a significant level with p -values of 0.0003 and 0.0004 for POD 4 and 7, respectively. Moreover, the collagen I/III ratio was also significantly different between the two groups either at POD 4 (0.88 ± 0.23 vs. 0.53 ± 0.08; p = 0.0003) and 7 (1.77 ± 0.65 vs. 1.03 ± 0.28; p = 0.004). Conclusions Monofilament non-absorbable sutures show a significantly higher collagen I/III ratio than multifilament absorbable sutures for abdominal fascia closure in rats. Our findings imply that the usage of monofilament non-absorbable sutures might have a beneficial effect on decreasing the incisional hernia occurrence.

The Use of Surgical Adhesive and Suture Fixing Meshes to the Abdominal Wall: An Experimental Study in Rats

“…According to the study by Sofii et al 19 , delays in defect healing can occur when type III collagen retards to decrease and type I collagen takes a longer time to spread. It was not observed in the present study.…”

“…During the healing process, type III collagen is replaced by type I collagen, which is highly resistant. Therefore, the relations between types I and III collagen have a great influence on many parameters of the healing process 19 .…”

Comparing the effect of tissue adhesive and suturing material on collagen I/III ratio in abdominal skin wounds: an experimental study

“…All samples were obtained and incised at the Anatomy Department of our institution. The methods used in this study were based on those employed in a previous study conducted by our group 8 . The rats were sedated with an intramuscular injection of 60 mg/kg after aseptic-antiseptic action with 1% povidone iodine prior to the incision of the wound.…”

Occurrence and prevention of incisional hernia following laparoscopic colorectal surgery

“…Calaluce et al [ 71 ] conducted a genome-wide microarray analysis, revealing expression differences in 174 genes in the skin and fascia of patients with non-IH and recurrent IH postoperatively. Notably, eight genes, including PCOLCE2, COL1A1 , and COL3A1 , directly involved in collagen synthesis, further confirm the decrease in collagen I/III ratio in the skin and fascia of patients with IH[ 72 ]. Additionally, Böhm et al [ 73 ] employed antibody microarrays to identify 25 proteins with differential expression levels in the preoperative plasma of patients with IH, related to wound healing, inflammatory factors, and cell adhesion.…”